Insect Gut Bacteria Promoting the Growth of Tomato Plants (Solanum lycopersicum L.)

Krawczyk, Krzysztof; Szabelska‐Berȩsewicz, Alicja; Przemieniecki, Sebastian Wojciech; Szymańczyk, Mateusz; Obrępalska‐Stęplowska, Aleksandra

doi:10.3390/ijms232113548

Cited by 5 publications

(1 citation statement)

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“…(Table S3). Phosphates participate in metabolic processes and many reactions of phosphates are involved in energy metabolism [41,42]. Our dates indicate the involvement of twelve phosphate-related proteins in the midgut content, pointing towards active energy conversion during the pupal stage (Table S3).…”

Section: Proteins Involved In Metabolismmentioning

confidence: 66%

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage

Wang,

Yang,

Tian

et al. 2023

Insects

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The silkworm Bombyx mori, a lepidopteran insect, possesses an 8–10-day pupal stage, during which significant changes occur in the midgut, where it first condenses into the yellow body, and then undergoes decomposition. To gain insights into this transformation process, proteomics was performed on Bombyx mori midgut contents on day 2 and day 7 after pupation. The results revealed the identification of 771 proteins with more than one unique peptide. An analysis using AgriGO demonstrated that these proteins were predominantly associated with catalytic activity. Among the identified proteins, a considerable number were found to be involved in carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleic acid degradation, and energy support. Additionally, variations in the levels of certain proteases were observed between the midgut contents on day 2 and day 7 after pupation. An in-depth analysis of the two-dimensional electrophoresis of the midgut contents on day 7 after pupation led to the identification of twelve protein spots with potential gelatinolytic activity. Among these, six proteases were identified through mass spectrometry, including the p37k protease, vitellin-degrading protease, chymotrypsin-2, etc. These proteases may be responsible for the digestion of the yellow body during the later stages of pupal development.

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Section: Proteins Involved In Metabolismmentioning

confidence: 66%