Insect-specific viruses: from discovery to potential translational applications

Nouri, Shahideh; Matsumura, Emilyn E.; Kuo, Yen-Wen; Falk, Bryce W.

doi:10.1016/j.coviro.2018.07.006

Cited by 95 publications

(91 citation statements)

References 97 publications

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“…Finally, our results show the importance of taking EVEs into account in metaviromic studies (NOURI et al , 2018) . Some of the chuvirus EVEs detected in our study exhibited a high degree of identity (99.08 to 100%) with previously described circulating chuviruses Figure 6: General scheme of the chuvirus derived EVE integration into the germinative cells of the host genome, phusion with Pao retroelements and potential antiviral mechanisms by receptor competition.…”

Section: Discussionmentioning

confidence: 56%

In and outs of Chuviridae endogenous viral elements: origin of a retrovirus and signature of ancient and ongoing arms race in mosquito genomes

Dezordi

Vasconcelos

Rezende

et al. 2020

Preprint

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Background: Endogenous viral elements (EVEs) are sequences of viral origin integrated into the host genome. EVEs have been characterized in various insect genomes, including mosquitoes. A large EVE content has been found in Aedes aegypti and Aedes albopictus genomes among which a recently described Chuviridae viral family is of particular interest, owing to the abundance of EVEs derived from it, the discrepancy in the endogenized gene regions and the frequent association with retrotransposons from the BEL-Pao superfamily. In order to better understand the endogenization process of chuviruses and the association between chuvirus glycoproteins and BEL-Pao retrotransposons, we performed a comparative genomics and evolutionary analysis of chuvirus-derived EVEs found in 37 mosquito genomes. Results: We identified 428 EVEs belonging to the Chuviridae family confirming the wide discrepancy between the number of genomic regions endogenized: 409 glycoproteins, 18 RNA-dependent RNA polymerases and one nucleoprotein region. Most of the glycoproteins (263 out of 409) are associated specifically with retroelements from the Pao family. Focusing only on well assembled Pao retroelement copies, we estimated that 263 out of 379 Pao elements are associated with chuvirus-derived glycoproteins. Seventy-three potentially active Pao copies were found to contain glycoproteins into their LTR boundaries.Thirteen out of these were classified as complete and likely autonomous copies, with a full

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Section: Discussionmentioning

confidence: 56%

In and outs of Chuviridae endogenous viral elements: origin of a retrovirus and signature of ancient and ongoing arms race in mosquito genomes

Dezordi

Vasconcelos

Rezende

et al. 2020

Preprint

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“…Viruses associated with pest insects have been described using classic approaches and metagenomic analyses, the latter being a more sensitive method to reveal the ubiquity and diversity of insect viruses (Liu et al, 2011). Viruses belonging to the Baculoviridae, Parvoviridae, Flaviviridae, Ascoviridae, Togaviridae, Bunyavirales, and Rhabdoviridae have most commonly been associated with insects, but many new insect viruses have been discovered with metagenomic approaches (Nouri et al, 2018). There are still many questions that remain unanswered in terms of diversity of the viruses, the predominance of viruses over the life cycle, and their impact on the physiology and life history of pest insects.…”

Section: Virusesmentioning

confidence: 99%

“…The composition of the viral part of the microbiome, the virome (i.e., the community of viruses in a given host or environment), has also been extensively studied for various mosquito species (e.g., Aedes spp., Culex spp. ), in order to characterize the disease-causing viruses as well as the insect-specific viruses (Atoni et al, 2018;Nouri et al, 2018). Interestingly, there is growing evidence that the vector competence of the insect can be affected by the insect-specific viruses they also carry; that is, several of the insect-specific viruses have been shown to suppress or enhance the transmission of medically important viruses (Hobson-Peters et al, 2013;Hall-Mendelin et al, 2016;Zhang et al, 2017).…”

Section: Vectoring Of Virusesmentioning

confidence: 99%

The microbiome of pest insects: it is not just bacteria

Gurung

Wertheim

Salles

2019

Entomologia Exp Applicata

155

113

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Insects are associated with multiple microbes that have been reported to influence various aspects of their biology. Most studies in insects, including pest species, focus on the bacterial communities of the microbiome even though the microbiome consists of members of many more kingdoms, which can also have large influence on the life history of insects. In this review, we present some key examples of how the different members of the microbiome, such as bacteria, fungi, viruses, archaea, and protozoa, affect the fitness and behavior of pest insects. Moreover, we argue that interactions within and among microbial groups are abundant and of great importance, necessitating the use of a community approach to study microbial–host interactions. We propose that the restricted focus on bacteria very likely hampers our understanding of the functioning and impact of the microbiome on the biology of pest insects. We close our review by highlighting a few open questions that can provide an in‐depth understanding of how other components of the microbiome, in addition to bacteria, might influence host performance, thus contributing to pest insect ecology.

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“…This indicated complete proteolytic processing of the GFP-VP2 peptide in the case of both DWV-L-GFP and DWV-S-GFP ( Figure 3A) suggesting that maturation of the structural proteins capable of packaging DWV genomic RNA took place during replication of the egfp-tagged DWV. 7…”

Section: Infectivity the Cdna Clone-derived Gfp-tagged Dwv In Honey Bmentioning

confidence: 99%

“…Insect-specific RNA virus vectors have been designed using genomes of the arboviruses, members of Flavivirus, Alphavirus and Nodavirus groups [1,7,8]. Currently, there are no RNA vectors based on genomes of viruses naturally infecting honey bees.…”

Section: Introductionmentioning

confidence: 99%

Development of a honey bee RNA virus vector based on the genome of Deformed wing virus

Ryabov

Christmon

Heerman

et al. 2020

Preprint

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We developed a honey bee RNA-virus vector based on the genome of a picorna-like Deformed wing virus (DWV), the main viral pathogen of the honey bee (Apis mellifera). To test the potential of DWV to be utilized as a vector, the 717 nt sequence coding for the enhanced green fluorescent protein (eGFP), flanked by the peptides targeted by viral protease, was inserted into an infectious cDNA clone of DWV in-frame between the leader protein and the virus structural protein VP2 genes. The in vitro RNA transcripts from egfp-tagged DWV cDNA clones were infectious when injected into honey bee pupae. Stable DWV particles containing genomic RNA of the recovered DWV with egfp inserts were produced, as evidenced by cesium chloride density gradient centrifugation. These particles were infectious to honey bee pupae when injected intra-abdominally. Fluorescent microscopy showed GFP expression in the infected cells and Western blot analysis demonstrated accumulation of free eGFP rather than its fusions with DWV LP and/or VP2 proteins. Analysis of the progeny egfp-tagged DWV showed gradual accumulation of genome deletions for egfp, providing estimates for the rate of loss of a non-essential gene an insect RNA virus genome during natural infection.

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Insect-specific viruses: from discovery to potential translational applications

Cited by 95 publications

References 97 publications

In and outs of Chuviridae endogenous viral elements: origin of a retrovirus and signature of ancient and ongoing arms race in mosquito genomes

In and outs of Chuviridae endogenous viral elements: origin of a retrovirus and signature of ancient and ongoing arms race in mosquito genomes

The microbiome of pest insects: it is not just bacteria

Development of a honey bee RNA virus vector based on the genome of Deformed wing virus

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