Insensitivity to Ethylene Conferred by a Dominant Mutation in
            <i>Arabidopsis thaliana</i>

Bleecker, Anthony B.; Estelle, Mark; Somerville, Chris; Kende, Hans

doi:10.1126/science.241.4869.1086

Cited by 913 publications

(623 citation statements)

References 29 publications

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“…While the functions of LeETR genes have not been established, ample data support hormone receptor status for Arabidopsis ETR1 (Bleecker et al, 1988;Chang et al, 1993;Guzman and Ecker, 1990;. The significant structural identity between LeETR1, LeETR2, NR and Arabidopsis ETR1 suggests that these tomato homologs are likely to function as ethylene receptors.…”

Section: Discussionmentioning

confidence: 99%

Differential regulation of the tomato ETR gene family throughout plant development

1998

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SummaryEthylene perception in plants is co-ordinated by multiple hormone receptor candidates sharing sequence commonalties with prokaryotic environmental sensor proteins known as two-component regulators. Two tomato homologs of the Arabidopsis ethylene receptor ETR1 were cloned from a root cDNA library. Both cDNAs, termed LeETR1 and LeETR2, were highly homologous to ETR1, exhibiting~90% deduced amino acid sequence similarity and 80% deduced amino acid sequence identity. LeETR1 and LeETR2 contained all the major structural elements of two-component regulators, including the response regulator motif absent in LeETR3, the gene encoding tomato NEVER RIPE (NR). Using RNase protection analysis, the mRNAs of LeETR1, LeETR2 and NR were quantified in tissues engaged in key processes of the plant life cycle, including seed germination, shoot elongation, leaf and flower senescence, floral abscission, fruit set and fruit ripening. LeETR1 was expressed constitutively in all plant tissues examined. LeETR2 mRNA was expressed at low levels throughout the plant but was induced in imbibing tomato seeds prior to germination and was down-regulated in elongating seedlings and senescing leaf petioles. NR expression was developmentally regulated in floral ovaries and ripening fruit. Notably, hormonal regulation of NR was highly tissue-specific. Ethylene biosynthesis induced NR mRNA accumulation in ripening fruit but not in elongating seedlings or in senescing leaves or flowers. Furthermore, the abundance of mRNAs for all three LeETR genes remained uniform in multiple plant tissues experiencing marked changes in ethylene sensitivity, including the cell separation layer throughout tomato flower abscission.

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Section: Discussionmentioning

confidence: 99%

Differential regulation of the tomato ETR gene family throughout plant development

1998

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“…5c). Ethylene also acts an endogenous regulator of various morphogenic processes, including the determination of the size of organs (Bleecker et al 1998), and inhibition is often associated with the action of ethylene and/or peroxidases (Abeles et al 1992). Our results are consistent with this observation, since shoot length significantly increased on media containing kinetin.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

2007

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The interaction between plant hormones throughout plant regeneration and development is complex. In the present study, we extended our investigation and disclosed the capacity of de novo shoot organogenesis from cotyledon explants of bottle gourd in relation to plant hormones and ethylene inhibitor. Synergistic effect of kinetin and benzyl adenine (BA) enhanced the shoot regeneration efficiency (80.6%) from cotyledon explants of bottle gourd, compared with BA or kinetin used separately, even in the presence of AgNO 3 . The use of BA or kinetin separately or in synergistic combination led to a characteristic brownish color of the explants at the bud formation stage which was related to ethylene production. In the cotyledon explants cultured on MS medium supplemented with kinetin, the production of ethylene was lower than when BA or the combination of BA and kinetin was used. However, the regeneration capacity was very low and the emergence of shoot buds was delayed. BA promoted ethylene production while the synergistic effect of kinetin and BA decreased ethylene production and played a role in high frequency of regeneration from cotyledon explants of bottle gourd. The physiological relevance of the hormonal interaction in relation to the regulation of ethylene action and effect on regeneration was discussed.

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“…More than a decade ago, additional components downstream of signaling molecules SA, JA and ethylene were characterized in several mutant plants, such as the jasmonate response mutant jar [26], the ethylene response mutant etr [27] and the SAR regulatory mutant npr1 [28]. In a genetic screening for plants failed to express PR genes after SAR induction, npr1 mutant was isolated in Arabidopsis [28] and NPR1 gene was cloned [29].…”

Section: Introductionmentioning

confidence: 99%

OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1

et al. 2005

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WRKY family proteins are a class of plant specific transcription factors that involve in many stress response pathways. It has been shown that one Arabidopsis WRKY protein, AtWRKY29/22, is activated by MAP kinase signaling cascade and confers resistance to both bacterial and fungal pathogens. However, little is known about the biological roles of WRKY proteins in rice. In this study, we investigated the expression patterns of rice AtWRKY29/22 homolog, OsWRKY03, under different conditions, and also its possible role involved in plant defense. Our results showed that OsWRKY03 was up-regulated by several defense signaling molecules or different treatments. Further analysis revealed that the expression of OsWRKY03 was light dependent. Transcriptional activation activity of OsWRKY03 was also demonstrated by yeast functional assay. Transient expression of OsWRKY03-GFP fusion protein in onion epidermis cells showed that OsWRKY03 was a nuclear localized protein. OsNPR1 as well as several other pathogenesis-related genes, such as OsPR1b, phenylalanine ammonia-lyase (ZB8) and peroxidase (POX22.3), were induced in OsWRKY03-overexpressing transgenic plants. These results indicated that OsWRKY03 is located upstream of OsNPR1 as a transcriptional activator in salicylic acid (SA)-dependent or jasmonic acid (JA)-dependent defense signaling cascades.

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Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana

Cited by 913 publications

References 29 publications

Differential regulation of the tomato ETR gene family throughout plant development

Differential regulation of the tomato ETR gene family throughout plant development

Synergistic Effect of Kinetin and Benzyl Adenine Plays a Vital Role in High Frequency Regeneration from Cotyledon Explants of Bottle Gourd (Lagenaria siceraria) in Relation to Ethylene Production

OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1

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