Insertional mutagenesis with Tos17 for functional analysis of rice genes

Hirochika, Hirohiko

doi:10.1270/jsbbs.60.486

Cited by 29 publications

(19 citation statements)

References 68 publications

(50 reference statements)

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“…Nipponbare) straw in which the expression of genes encoding enzymes in the cinnamate/monolignol pathway were manipulated. is method is very rapid, nondestructive, and high-sensitive, and can be expanded to the screening of a large mutant collections, such as Tos mutant panel (Hirochika 2001(Hirochika , 2010.…”

Section: Resultsmentioning

confidence: 99%

Rapid analysis of transgenic rice straw using near-infrared spectroscopy

Hattori

Murakami

Mukai

et al. 2012

Plant Biotechnology

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A rapid near-infrared (NIR) spectroscopy method was established to predict the lignin and starch contents and enzymatic sacchari cation e ciency of transgenic rice (Oryza sativa cv. Nipponbare) straw wherein expression of genes encoding lignin synthetic enzymes are regulated. Strong correlations were obtained between laboratory wet chemistry values and the NIR-predicted values. is method is useful to develop transgenic rice where cell-wall formation is engineered.

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Section: Resultsmentioning

confidence: 99%

Rapid analysis of transgenic rice straw using near-infrared spectroscopy

Hattori

Murakami

Mukai

et al. 2012

Plant Biotechnology

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“…Insertional mutagenesis with Tos17 has been shown to be a useful tool for functional analysis of rice genes associated with many agronomic and biological traits Hirochika, 2010). We investigated genes important to pollen development in rice using pollen semisterility mutants tagged by insertional mutagenesis with Tos17.…”

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confidence: 99%

COLLAPSED ABNORMAL POLLEN1Gene Encoding the Arabinokinase-Like Protein Is Involved in Pollen Development in Rice

et al. 2013

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We isolated a pollen-defective mutant, collapsed abnormal pollen1 (cap1), from Tos17 insertional mutant lines of rice (Oryza sativa). The cap1 heterozygous plant produced equal numbers of normal and collapsed abnormal grains. The abnormal pollen grains lacked almost all cytoplasmic materials, nuclei, and intine cell walls and did not germinate. Genetic analysis of crosses revealed that the cap1 mutation did not affect female reproduction or vegetative growth. CAP1 encodes a protein consisting of 996 amino acids that showed high similarity to Arabidopsis (Arabidopsis thaliana) L-arabinokinase, which catalyzes the conversion of L-arabinose to L-arabinose 1-phosphate. A wild-type genomic DNA segment containing CAP1 restored mutants to normal pollen grains. During rice pollen development, CAP1 was preferentially expressed in anthers at the bicellular pollen stage, and the effects of the cap1 mutation were mainly detected at this stage. Based on the metabolic pathway of L-arabinose, cap1 pollen phenotype may have been caused by toxic accumulation of L-arabinose or by inhibition of cell wall metabolism due to the lack of UDP-L-arabinose derived from L-arabinose 1-phosphate. The expression pattern of CAP1 was very similar to that of another Arabidopsis homolog that showed 71% amino acid identity with CAP1. Our results suggested that CAP1 and related genes are critical for pollen development in both monocotyledonous and dicotyledonous plants.

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“…Several transposable elements (TEs) have been used for insertional mutagenesis in plants, including the Activator and Mutator transposons in maize (Zea mays; Walbot, 1992), the Tam3 transposon in Antirrhinum majus (Luo et al, 1991), the Tos17 retrotransposon in rice (Hirochika, 2010), and the LORE1 retrotransposon in Lotus japonicus (Fukai et al, 2012). Most remarkably, the Tnt1 retrotransposon, originally identified in tobacco (Nicotiana tabacum; Grandbastien et al, 1989), has been successfully used in insertional mutagenesis in several heterologous species, including Arabidopsis (Courtial et al, 2001), Medicago truncatula (d 'Erfurth et al, 2003), lettuce (Lactuca sativa; Mazier et al, 2007), and soybean (Glycine max; Cui et al, 2013).…”

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Insertional Mutagenesis Using Tnt1 Retrotransposon in Potato

Duangpan

Zhang

et al. 2013

Plant Physiol.

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Insertional mutagenesis using transfer DNA or transposable elements, which is an important tool in functional genomics and is well established in several crops, has not been developed in potato (Solanum tuberosum). Here, we report the application of the tobacco (Nicotiana tabacum) Tnt1 retrotransposon as an insertional mutagen in potato. The Tnt1 retrotransposon was introduced into a highly homozygous and self-compatible clone, 523-3, of the diploid wild potato species Solanum chacoense. Transposition of the Tnt1 elements introduced into 523-3 can be efficiently induced by tissue culture. Tnt1 preferentially inserted into genic regions in the potato genome and the insertions were stable during sexual reproduction, making Tnt1 an ideal mutagen in potato. Several distinct phenotypes associated with plant stature and leaf morphology were discovered in mutation screening from a total of 38 families derived from Tnt1-containing lines. We demonstrate that the insertional mutagenesis system based on Tnt1 and the 523-3 clone can be expanded to the genome-wide level to potentially tag every gene in the potato genome.

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Insertional mutagenesis with Tos17 for functional analysis of rice genes

Cited by 29 publications

References 68 publications

Rapid analysis of transgenic rice straw using near-infrared spectroscopy

Rapid analysis of transgenic rice straw using near-infrared spectroscopy

COLLAPSED ABNORMAL POLLEN1Gene Encoding the Arabinokinase-Like Protein Is Involved in Pollen Development in Rice

Insertional Mutagenesis Using Tnt1 Retrotransposon in Potato

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