2021
DOI: 10.1038/s42003-021-01901-z
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Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions

Abstract: Mammalian three-dimensional (3D) enteroids mirror in vivo intestinal organisation and are powerful tools to investigate intestinal cell biology and host–pathogen interactions. We have developed complex multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts. These avian enteroids develop optimally in suspension without the structural support required to produce mammalian enteroids, resulting in an inside-out enteroid conformation with media-facing apical brush borders. Histological… Show more

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Cited by 62 publications
(92 citation statements)
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“…Intestinal crypts sourced from mice and humans have been successfully cultured in vitro and are becoming increasingly popular in the exploration of intestinal development. Most studies have attempted to identify an ideal method for the culture of chicken intestinal organoid ( Pierzchalska et al., 2012 ; Powell and Behnke, 2017 ; Li et al., 2018 ; Acharya et al., 2020 ; Nash et al., 2021 ). Here, we successfully applied chicken serum and A8301, SB202190, VPA and CHIR99021 to promote chicken intestinal organoid formation.…”
Section: Discussionmentioning
confidence: 99%
“…Intestinal crypts sourced from mice and humans have been successfully cultured in vitro and are becoming increasingly popular in the exploration of intestinal development. Most studies have attempted to identify an ideal method for the culture of chicken intestinal organoid ( Pierzchalska et al., 2012 ; Powell and Behnke, 2017 ; Li et al., 2018 ; Acharya et al., 2020 ; Nash et al., 2021 ). Here, we successfully applied chicken serum and A8301, SB202190, VPA and CHIR99021 to promote chicken intestinal organoid formation.…”
Section: Discussionmentioning
confidence: 99%
“…Generating well-characterised ex vivo models of the chicken intestine is an essential advancement in poultry research to study their physiology and disease states, to improve health and minimise the number of animal studies. Recent advances in the generation of 3D chicken enteroids have improved the complexity of the cultured organoids to closer resemble native tissue [ 25 ], but 2D chicken intestinal models are still relatively primitive with partial characterisation of epithelial cell types, lacking the multi-cellular interactions found in vivo, and with limited scope for functional studies [ 17 24 , 27 ]. Here, we describe the generation of a confluent 2D epithelial layer from ED18 embryos with all epithelial cell lineages and a sub-epithelial mesenchymal layer, which mirror the architecture of the intestinal lining in vivo.…”
Section: Discussionmentioning
confidence: 99%
“…Tissue was isolated as previously described [ 25 ]. In brief, freshly removed chicken intestine (duodenum, jejunum, ileum and caeca) from ED18 Hy-Line Brown embryos was cut open longitudinally and then transversely into 5 mm pieces.…”
Section: Methodsmentioning
confidence: 99%
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