Insight into Compatible Solutes from Halophiles: Exploring Significant Applications in Biotechnology

Physiological metabolic topology analysis of Halomonas elongata DSM 2581T in response to sodium chloride stress

“…Noteworthy, the contents of glutamine were significantly higher than other amino acids, glutamine content reached the highest levels at 362.3 ± 49.9 mg/g DCW and 312.3 ± 9.9 mg/g DCW under 8% and 13% NaCl, which were 1.7‐ and 1.5 ‐fold higher than that under 1% NaCl, respectively (Supporting Information: Figure S2). Glutamine was found to accumulate in Methanosarcina thermophila , Methanogenium cariaci , and species of Methanohalophilus belonging to extremely halophilic archaebacteria (Jadhav et al, 2018), suggesting that glutamine might function as an osmoprotectant in H. elongata DSM 2581 T . In addition, glutamic acid was also reported as a key intermediate of several metabolic pathways, most notably in the γ‐glutamyl cycle through which the antioxidant glutathione is generated, glutamine was involved in the regulation of cell proliferation, inhibition of apoptosis, and cell signaling (DeBerardinis & Cheng, 2010).…”

“…Noteworthy, the contents of glutamine were significantly higher than other amino acids, glutamine content reached the highest levels at 362.3 ± 49.9 mg/g DCW and 312.3 ± 9.9 mg/g DCW under 8% and 13% NaCl, which were 1.7-and 1.5 -fold higher than that under 1% NaCl, respectively (Supporting Information: Figure S2). Glutamine was found to accumulate in Methanosarcina thermophila, Methanogenium cariaci, and species of Methanohalophilus belonging to extremely halophilic archaebacteria (Jadhav et al, 2018)…”

Betaine accumulation suppresses the de-novo synthesis of ectoine at a low osmotic concentration in Halomonas sp SBS 10, a bacterium with broad salinity tolerance

“…Richness and evenness of the Halomonas population are mainly influenced by salinity and pH [1,5]. To thrive in these conditions, species of the genus Halomonas have evolved a strategy of accumulating or synthesizing low molecular weight organic compatible solutes that do not interfere with cell metabolism [6][7][8][9].…”

Whole-Genome Shotgun Sequence of Halomonas sp. Strain SBS 10, Isolated from a Hypersaline Lake in India

“…H ypersaline environments harbor large numbers of halotolerant and moderately and extremely halophilic species that are exposed to severe stresses such as high osmolarity and low water activity (1,2). Along with several other bacterial species, species of the genus Halomonas are widely distributed throughout hypersaline environments.…”