Insight into isolation and characterization of compounds of Chaerophyllum bulbosum aerial part with antioxidant, anticholinesterase, anti-urease, anti-tyrosinase, and anti-diabetic activities

Molo, Zeynep; Tel‐Çayan, Gülsen; Deveci, Ebru; Duru, Mehmet Emin

doi:10.1016/j.fbio.2021.101201

Cited by 8 publications

(9 citation statements)

References 59 publications

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“…GS-320 columns were used to purify quercetin-3-O-β-D-glucopyranoside. Luteolin-7-O-β-D-glucopyranoside and quercetin-3-O-β-D-glucopyranoside exhibited higher DPPH • and ABTS •+ scavenging activities compared to BHA and α-tocopherol standards [38].…”

Section: High Performance Liquid Chromatographymentioning

confidence: 87%

“…Recycle HPLC is an effective method for separating compounds that elute close together, and the system's ability to remove contaminants between chromatography cycles ensures the high purity of the isolated compounds [98]. Molo et al [38] used C18, GS-320 columns to purify phenolic compounds from a subfraction of Chaerophyllum bulbosum extract. For the C18 column, they purified luteolin-7-O-β-D-glucopyranoside, a flavonoid compound, with methanol and water (50:50, v:v) as a mobile phase.…”

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

“…It is also an essential method for obtaining compounds that are hard to synthesize or for which there are no commercial standards. Many isolation methods are used to isolate phenolic compounds that have antioxidant activity, including column chromatography [37], highperformance liquid chromatography (HPLC) [38], medium-pressure liquid chromatography (MPLC) [39], centrifugal partition chromatography (CPC) [40], high-speed counter-current chromatography (HSCCC) [41], and high-performance counter-current chromatography (HPCCC) [42]. Molecularly imprinted polymer techniques (MITs) have also been developed to selectively isolate phenolic compounds from extracts producing synthetic polymeric materials with homologous adsorption sites to a template molecule [43].…”

Section: Introductionmentioning

confidence: 99%

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Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Susanti,

Pratiwi,

Rosandi

et al. 2024

Plants

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In recent years, discovering new drug candidates has become a top priority in research. Natural products have proven to be a promising source for such discoveries as many researchers have successfully isolated bioactive compounds with various activities that show potential as drug candidates. Among these compounds, phenolic compounds have been frequently isolated due to their many biological activities, including their role as antioxidants, making them candidates for treating diseases related to oxidative stress. The isolation method is essential, and researchers have sought to find effective procedures that maximize the purity and yield of bioactive compounds. This review aims to provide information on the isolation or separation methods for phenolic compounds with antioxidant activities using column chromatography, medium-pressure liquid chromatography, high-performance liquid chromatography, counter-current chromatography, hydrophilic interaction chromatography, supercritical fluid chromatography, molecularly imprinted technologies, and high-performance thin layer chromatography. For isolation or purification, the molecularly imprinted technologies represent a more accessible and more efficient procedure because they can be applied directly to the extract to reduce the complicated isolation process. However, it still requires further development and refinement.

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Section: High Performance Liquid Chromatographymentioning

confidence: 87%

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Susanti,

Pratiwi,

Rosandi

et al. 2024

Plants

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“…23 Macrolepiota dolichaula polysaccharide was obtained as highly antioxidant active in hydroxyl radical scavenging (∼50% inhibition at 800 μg/mL), superoxide radical scavenging (∼90% inhibition at 300 μg/mL), and β-carotene-linoleic acid (∼50% inhibition at 400 μg/mL) assays in the study of Samanta et al 48 Antioxidant activity of H. lucorum was reported as weak by reducing power (absorbance: ∼0.01), hydroxyl radical scavenging (∼5% inhibition), DPPH • scavenging (∼20% inhibition), and superoxide anion radical scavenging (∼15% inhibition) assays at 10.0 mg/mL. 39 Antioxidant activities of CCPs-1 and CCPs-2 polysaccharides of C. cornucopioides were defined as moderate by using DPPH 18 High DPPH • (81.2% inhibition at 400 μg/mL) and ABTS •+ (99.4% inhibition at 500 μg/mL) scavenging activities were found in the C. cornucopioides polysaccharide fraction. 26 The antioxidant activity results of the studied polysaccharide extracts and literature data supported each other.…”

Section: Monosaccharide Compositionmentioning

confidence: 99%

Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities

Deveci,

Tel-Çayan,

Çayan

et al. 2024

ACS Omega

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Mushroom polysaccharides are important bioactive compounds derived from mushrooms with various beneficial properties. In this study, the chemical characterization and bioactivities of polysaccharide extracts from four different edible mushrooms, Clavariadelphus truncatus Donk, Craterellus tubaeformis (Fr.) Queĺ., Hygrophorus pudorinus (Fr.) Fr., and Macrolepiota procera (Scop.) Singer were studied. Glucose (13.24−56.02%), galactose (14.18−64.05%), mannose (2.18−18.13%), fucose (1.21−5.78%), and arabinose (0.04−5.43%) were identified in all polysaccharide extracts by GC-MS (gas chromatography−mass spectrometry). FT-IR (Fourier transform infrared spectroscopy) confirmed the presence of characteristic carbohydrate patterns. 1 H NMR suggested that all polysaccharide extracts had αand β-Dmannopyranose, D-glucopyranose, D-galactopyranose, α-L-arabinofuranose, and α-L-fucopyranose residues. Approximate molecular weights of polysaccharide extracts were determined by HPLC (high-performance liquid chromatography). The best antioxidant activity was found in M. procera polysaccharide extract in DPPH • (1,1-diphenyl-2-picrylhydrazyl) scavenging (39.03% at 800 μg/ mL), CUPRAC (cupric reducing antioxidant capacity) (A 0.50 : 387.50 μg/mL), and PRAP (phosphomolybdenum reducing antioxidant power) (A 0.50 : 384.08 μg/mL) assays. C. truncatus polysaccharide extract showed the highest antioxidant activity in ABTS •+ scavenging (IC 50 : 734.09 μg/mL), β-carotene-linoleic acid (IC 50 : 472.16 μg/mL), and iron chelating (IC 50 : 180.35 μg/mL) assays. Significant anticancer activity was found in C. truncatus polysaccharide extract on HT-29 (IC 50 : 46.49 μg/mL) and HepG2 (IC 50 : 48.50 μg/mL) cell lines and H. pudorinus polysaccharide extract on the HeLa cell line (IC 50 : 51.64 μg/mL). Also, H. pudorinus polysaccharide extract possessed prominent AChE (acetylcholinesterase) inhibition activity (49.14% at 200 μg/mL).

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“…The AchE inhibitory activities of all isolates (1 a/1 b-4 a/4 b) were performed by Ellman assay following the previously described, [42][43] and the detailed procedures were described in the SI.…”

Section: Ache Inhibitory Activity Assaymentioning

confidence: 99%

Four Pairs of Neuroprotective Aryldihydronaphthalene‐Type Lignanamide Enantiomers from the Herbs of Solanum lyratum

Mi,

Chang,

Zhang

et al. 2023

Chemistry & Biodiversity

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Four pairs of aryldihydronapthalene‐type lignanamide enantiomers were isolated from Solanum lyratum (Solanaceae). The enantiomeric separation was accomplished by chiral‐phase HPLC, and five undescribed compounds were elucidated. Analysis by various spectroscopy and ECD calculations, the structures of undescribed compounds were illuminated. The neuroprotective effects of all compounds were evaluated using H2O2‐induced human neuroblastoma SH‐SY5Y cells and AchE inhibition activity. Among them, compound 4a exhibited remarkable neuroprotective effects at high concentrations of 25 and 50 μmol/L comparable to Trolox. Compound 1a showed the highest AchE inhibition with the IC50 value of 3.06 ± 2.40 μmol/L. Molecular docking of the three active compounds was performed and the linkage between the compounds and the active site of AchE was elucidated.

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Insight into isolation and characterization of compounds of Chaerophyllum bulbosum aerial part with antioxidant, anticholinesterase, anti-urease, anti-tyrosinase, and anti-diabetic activities

Cited by 8 publications

References 59 publications

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Separation Methods of Phenolic Compounds from Plant Extract as Antioxidant Agents Candidate

Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities

Four Pairs of Neuroprotective Aryldihydronaphthalene‐Type Lignanamide Enantiomers from the Herbs of Solanum lyratum

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