Insight into Isolation, Identification and Antimicrobial Sensitivity of some Moulds Isolated from Fresh Water Fishes

Tawab, Ashraf A. Abd El; Hofy, Fatma I. El; Moustafa, Eman M.; Halawa, Marwa

doi:10.17582/journal.aavs/2020/8.2.174.182

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…Fungi are opportunistic pathogens When infecting freshwater fish, they may cause high mortalities, hinder the growth rate and reduce hatchability especially in chronic infection, including mycotoxins production when fish are subjected to stress of any type such as poor water quality (Abd El Ghany & Elias, 2014;Abd El-Tawab et al, 2020;Noor El-Deen et al, 2018). One of the feed contaminants is the Aspergillus species, which produces aflatoxins (Ferreira et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The effect of dietary nanocurcumin on the growth performance, body composition, haemato‐biochemical parameters and histopathological scores of the Nile tilapia ( Oreochromis niloticus ) challenged with Aspergillus flavus

Eissa

Ezzo

Khalil

et al. 2022

Aquaculture Research

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The study aims to investigate the potential effect of nanocurcumin as feed additive in the diet of Oreochromis niloticus to improve its growth performance, health status and resistance against Aspergillus flavus. The control group was fed on a basal diet without nanocurcumin, and four diets T1, T2, T3 and T4 were supplemented with 10, 25, 40 and 55 mg/kg of nanocurcumin, respectively, in triplicate (20 fish/replicate). The duration of the feeding trial was 60 days. The final body weight, weight gain, specific growth rate and survival rate showed significantly (p < 0.05) increased values in the nanocurcumin groups than the control. Fish fed with nanocurcumin supplementation showed improvement in RBCs, haemoglobin, total protein, albumin and globulin while there was a decrease in the liver enzymes (AST and ALT), glucose and alkaline phosphatase. The creatinine was also decreased in fish fed nanocurcumin. The digestive enzymes amylase and lipase increased in the nanocurcumin‐treated groups, and the triglycerides values showed non‐significant increase, whereas the cholesterol values showed non‐significant decrease in T1 and T4. Meanwhile, the cortisol was nearly the same in all groups. At the end of the trial, the fish were challenged with Aspergillus flavus for 15 days. Aspergillus flavus resulted in the mortality of 100% of the control group and the groups with lower doses of nanocurcumin (T1 and T2) within the first week and second week post challenge respectively. In the treatments with high doses (T3 and T4), higher survival rates were recorded in a dose‐dependent manner. The pathogenicity of Aspergillus flavus was confirmed histopathologically. It was concluded that the dietary supplementation of nanocurcumin enhanced the health status of O. niloticus by improving the haemato‐immunological response and body composition parameters of the fish, and protected it from the Aspergillus flavus infection with optimum inclusion levels of 25–40 mg/kg diet.

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Section: Introductionmentioning

confidence: 99%

The effect of dietary nanocurcumin on the growth performance, body composition, haemato‐biochemical parameters and histopathological scores of the Nile tilapia ( Oreochromis niloticus ) challenged with Aspergillus flavus

Eissa

Ezzo

Khalil

et al. 2022

Aquaculture Research

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show abstract

“…However, others require environmental stresses such as malnutrition, unstable water temperature, poor water quality and overcrowding. Fungal infections are opportunistic and only affect fish when they are stressed by poor conditions, water quality problems and temperature fluctuations (El-Tawab et al, 2020).…”

Section: Identification Of Fungus From Diseased African Catfishmentioning

confidence: 99%

ISOLATION AND CHARACTERIZATION OF FUNGI ASSOCIATED WITH BODY AND EGG FROM DISEASED AFRICAN CATFISH, Clarias gariepinus

Iberahim,

Mohd Roslan,

Ahmad Arabi

et al. 2023

UMT JUR

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African catfish (Clarias gariepinus) are in high demand in the Malaysian market. However, these species are known to be easily infected with diseases such as fungi, which can cause mortality and economic losses. In addition, fungal infection in fish eggs can reduce their production. The objective of this study was to isolate and identify fungi from infected African catfish and their eggs. Symptoms include a swollen lesion, irregular swimming, darker discoloration, and screening of pathogenic fungi. Isolations, identifications, and extracellular enzymatic assays were performed for the isolated fungi. Potato dextrose agar was used as isolation medium for isolating fungi on African catfish and their eggs. Fungi were identified by macroscopic observation of growth morphology and their structure, followed by microscopic observation after staining with lactophenol cotton blue to observe hyphae. From the result, three fungi were isolated and identified as Mucor sp. (2n) and Trichoderma sp. (1n). All isolated fungi were also analyzed for extracellular enzymes such as proteases, amylases, and lipases to test their ability to produce them as pathogenic fungi. Trichoderma sp. showed positive results in all tests. However, one strain of Mucor sp. reacted positively to the amylase and lipase test, while the other strain of the same species showed no reaction, indicating a negative result. In conclusion, the isolated fungi have virulent properties and can cause mortality if the body and eggs are infected. Therefore, fish farms need comprehensive health management to prevent the spread of fungal diseases

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Efficiency of Persicaria salicifolia as a natural alternative antifungal against Aspergillus flavus infection in Oreochromis niloticus from Aswan Governorate, Egypt

El-Zayat,

Abdel-Motaal,

Mohamed

et al. 2024

Aquacult Int

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Fungal diseases in fish cause economic losses all over the world, and knowledge about them is scarce and outdated in Aswan Governorate, Egypt, making interpretation, prevention, and treatment difficult. The necessity to find a fungicide that is natural, environmentally friendly, and does not emerge drug resistance is a must. Therefore, the current study aimed to isolate and diagnose fungal infection in farmed Oreochromis niloticus, causing mortalities, in Aswan Governorate. During 2021, 200 fresh O. niloticus samples were collected from the Sahary Fish Hatchery and Aswan General Authority for Fish Resources Development fish farm. Some fish showed hemorrhagic lesions all over the body, detachment of scales, and fin erosion. Collected tissue samples were cultured on potato dextrose agar and Sabouraud dextrose agar for phenotypic characterization. Macroscopic and microscopic analyses were used to identify the isolated fungi. A total of 18 fungal species and two varieties appertaining to ten fungal genera were recovered from 48 samples out of 200 examined O. niloticus (24%), with Aspergillus flavus being the most prevalent at a rate of 25.6%. The isolated A. flavus was proven to be pathogenic to farmed O. niloticus, as by experimental infection. The natural herb Persicaria salicifolia had an LC50 value of 41.68 mg/l in exposed O. niloticus and was used to treat A. flavus-infected O. niloticus. It can be concluded that A. flavus poses a major hazard to O. niloticus aquaculture and can be treated with 40 mg/kg in feed or 20 mg/l in water of P. salicifolia for 6 days.

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Insight into Isolation, Identification and Antimicrobial Sensitivity of some Moulds Isolated from Fresh Water Fishes

Cited by 5 publications

References 30 publications

The effect of dietary nanocurcumin on the growth performance, body composition, haemato‐biochemical parameters and histopathological scores of the Nile tilapia ( Oreochromis niloticus ) challenged with Aspergillus flavus

The effect of dietary nanocurcumin on the growth performance, body composition, haemato‐biochemical parameters and histopathological scores of the Nile tilapia ( Oreochromis niloticus ) challenged with Aspergillus flavus

ISOLATION AND CHARACTERIZATION OF FUNGI ASSOCIATED WITH BODY AND EGG FROM DISEASED AFRICAN CATFISH, Clarias gariepinus

Efficiency of Persicaria salicifolia as a natural alternative antifungal against Aspergillus flavus infection in Oreochromis niloticus from Aswan Governorate, Egypt

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