“…33−36 However, for pure water, low-molarity aqueous solutions, and organic liquids, the contrast observed in 3D AFM data can be explained in terms of the liquid density variations through the interface (Figure 1b). 33,36,37 We apply tapping mode AFM 38 to quantify the surface roughness and uniformity of the pristine and functionalized silicon surfaces in water before 3D AFM imaging (Figure S1, Supporting Information). The root-mean square roughness of pristine, OTS, and 3-aminopropyltriethoxysilane (APTES)functionalized silica surfaces are, respectively, 0.5, 0.3, and 0.45 nm.…”