Insight into the self-assembly behavior of α-zein by multi-spectroscopic and molecular simulations: An example of combination with the main component of jujube peel pigments – Rutin

Zhu, Jinpeng; Li, Zhentian; Wu, Caie; Fan, Gongjian; Li, Tingting; Shen, Dongbei; Dou, Jing; Liang, Yi

doi:10.1016/j.foodchem.2022.134684

Cited by 18 publications

(9 citation statements)

References 40 publications

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“…Evidence showed that the complexes were uniformly spherical and accompanied by smooth surfaces (Figure S1A). Compared with the free JPP, the bioavailability of complexes has been significantly increased to 69.73% ± 5.06% (Figure S1B), which is consistent with previous findings …”

Section: Resultssupporting

confidence: 92%

“…The JPP/zein complexes were prepared through antisolvent methods according to our previous methods . According to the results of pre-experiments, the highest encapsulation efficiency of JPP/zein complexes was obtained when the mass ratio of JPP and zein reached 1:3.…”

Section: Methodsmentioning

confidence: 99%

“…Scanning electron microscopy (SEM) (Quanta 200, FEI, OH, USA) was applied for the ultrastructure characterization of JPP/zein complexes. The in vitro bioavailability of JPP/zein complexes was calculated after simulated digestion …”

Section: Methodsmentioning

confidence: 99%

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Intestinal Barrier Protective Study of Jujube Peel Polyphenols/Zein Complexes by a Combined Caco-2 Cell and Caenorhabditis elegans Model: A Perspective of Proteomics

Zhu

Dou

et al. 2023

J. Agric. Food Chem.

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Jujube peels have been recognized as a promising resource of several bioactive ingredients. The main composition of jujube peel polyphenols (JPP) has been identified as rutin, kaempferol-3-O-rutinosid, and salicylic acid. The JPP/zein complexes, whose bioavailability reached 69.73% ± 5.06% in vitro, have been formed successfully. The Caco-2 cell and Caenorhabditis elegans (C. elegans) models have been combined to detect the intestinal barrier protective effect of JPP and its complexes. Results showed that JPP/zein complexes contain better protection capability than JPP in both models. In the Caco-2 cell model, the complex relieved intestinal barrier damage by regulating the tight junction proteins. Moreover, the lysosome pathway has been activated, further regulating immune responses and lipid transportation, improving the barrier function of C. elegans after incubation with JPP/zein complexes according to the proteomics study. This work provides new insights into intestinal barrier protection with bioactive compounds.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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Intestinal Barrier Protective Study of Jujube Peel Polyphenols/Zein Complexes by a Combined Caco-2 Cell and Caenorhabditis elegans Model: A Perspective of Proteomics

Zhu

Dou

et al. 2023

J. Agric. Food Chem.

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“…It is speculated that a strong binding between zein and rutin contributed to the formation of a stable supramolecular complex (Figure 2). 38 …”

Section: Resultsmentioning

confidence: 99%

Investigation of the binding interactions mechanism between zein with chrysin by multispectroscopic techniques

Gao

Cheng

et al. 2023

J of Molecular Recognition

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As a natural carrier protein, zein was intensively studied for the construction of a flavonoid delivery system. Chrysin has presented superior tumor‐resistant, anti‐inflammatory, and anti‐oxidation potentials among the flavonoid candidates in clinical practice. However, due to inadequate research, the binding mechanism and structural affinity of zein to chrysin are still indeterminate. Therefore, multispectral methods were employed to explore the molecular interaction of zein and chrysin in this work. These techniques showed that chrysin reduced the intrinsic fluorescence of zein via a static process and that the interaction between zein and chrysin was mainly driven spontaneously by hydrophobic forces. Additionally, the experimental results revealed the changed microenvironment in the vicinity of tyrosine and affected secondary structure in the presence of chrysin, indicating zein's conformation were altered by chrysin. This work provided comprehensive insight into the combination of plant‐derived protein (zein) and flavonoids (chrysin) and helped rationalize the protection, transportation, and release of chrysin through a zein‐based delivery system.

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“…The initial 2D structures of LIG and AIV were obtained from the PubChem database (). The molecular dynamics process consisted of an equilibrium phase and a production phase, which were randomly assigned to different initial speeds according to the Maxwell–Boltzmann distribution . During the simulation, a 44.102 Å × 45.373 Å × 35.186 Å box was constructed and randomly filled with two LIG molecules and one AIV molecule to form the simulation system.…”

Section: Methodsmentioning

confidence: 99%

Insights into the Mechanism of Supramolecular Self-Assembly in the Astragalus membranaceus–Angelica sinensis Codecoction

Liang,

Bi,

Zhou

et al. 2023

ACS Appl. Mater. Interfaces

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Astragalus membranaceus (Fisch.) Bge. (AM) and Angelica sinensis (Oliv.) Diels (AS) constitute a classic herb pair in prescriptions to treat myocardial fibrosis. To date, research on the AM−AS herb pair has mainly focused on the chemical compositions associated with therapeutic efficacy. However, supermolecules actually exist in herb codecoctions, and their selfassembly mechanism remains unclear. In this study, supermolecules originating from AM−AS codoping reactions (AA-NPs) were first reported. The chemical compositions of AA-NPs showed a dynamic self-assembly process. AA-NPs with different decoction times had similar surface groups and amorphous states; however, the size distributions of these nanoparticles might be different. Taking the interaction between Z-ligustilide and astragaloside IV as an example to understand the self-assembly mechanism of AA-NPs, it was found that the complex could be formed with a molar ratio of 2:1. Later, AA-NPs were proven to be effective in the treatment of myocardial fibrosis both in vivo and in vitro, the in-depth mechanisms of which were related to the recovery of cardiac function, reduced collagen deposition, and inhibition of the endothelial-to-mesenchymal transition that occurred in the process of myocardial fibrosis. Thus, AA-NPs may be the chemical material basis of the molecular mechanism of the AM−AS decoction in treating isoproterenol-induced myocardial fibrosis. Taken together, this work provides a supramolecular strategy for revealing the interaction between effective chemical components in herb-pair decoctions.

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Insight into the self-assembly behavior of α-zein by multi-spectroscopic and molecular simulations: An example of combination with the main component of jujube peel pigments – Rutin

Cited by 18 publications

References 40 publications

Intestinal Barrier Protective Study of Jujube Peel Polyphenols/Zein Complexes by a Combined Caco-2 Cell and Caenorhabditis elegans Model: A Perspective of Proteomics

Intestinal Barrier Protective Study of Jujube Peel Polyphenols/Zein Complexes by a Combined Caco-2 Cell and Caenorhabditis elegans Model: A Perspective of Proteomics

Investigation of the binding interactions mechanism between zein with chrysin by multispectroscopic techniques

Insights into the Mechanism of Supramolecular Self-Assembly in the Astragalus membranaceus–Angelica sinensis Codecoction

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