Insight into the Structure Elucidation of Flavonoids ThroughUV-Visible Spectral Analysis of Quercetin Derivatives Using Shift Reagents

Bajracharya, Gan B.; Paudel, Mohan; Kc, Rajendra

doi:10.3126/jncs.v37i0.32147

Cited by 3 publications

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“…The methanol extract of E. crista-galli twigs was dissolved in water and partitioned with n-hexane and ethyl acetate. Subsequently, we selected the ethyl acetate fraction for further purification steps, due to the similar polarity between flavonoids and the solvent [13][14][15]. The flavonoid phytochemical test using NaOH gave a positive result, which supported our decision.…”

Section: Extraction and Isolationmentioning

confidence: 52%

Flavanones from Erythrina crista-galli Twigs and Their Antioxidant Properties Determined through In Silico and In Vitro Studies

et al. 2022

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Flavonoids are a secondary metabolite group with various bioactivities, such as antioxidants. They are rich in the genus Erythrina, such as Erythrina crista-galli. This research aims to isolate and characterize flavonoids from the twigs of E. crista-galli and determine their antioxidant properties through in silico and in vitro assays. The ethyl acetate extract of E. crista-galli twigs were separated by column chromatography and characterized using spectroscopic methods. Density functional theory (DFT) calculations were performed on the isolated flavonoids and the reference compounds (ascorbic acid and quercetin) to obtain global descriptive parameters and a donor–acceptor map (DAM). We successfully isolated lupinifolin (1) and citflavanone (2) for the first time from E. crista-galli, along with lonchocarpol A (3), which has been discovered previously. The DAM suggests that these flavanones are good antiradicals with effective electron donors. However, they tend to be electron acceptors in methanol. The frontier molecular orbital analysis implies that lupinifolin (1) is a better antiradical than the other flavanones. The DPPH assays show that lupinifolin (1) has the highest antioxidant (antiradical) activity, with an IC50 value of 128.64 ppm. The in silico studies showed similar trends to the in vitro assays using the DPPH method.

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Section: Extraction and Isolationmentioning

confidence: 52%

Flavanones from Erythrina crista-galli Twigs and Their Antioxidant Properties Determined through In Silico and In Vitro Studies

et al. 2022

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“…Comparing the data of the scientific literature, it can be assumed that the addition of acetic acid salts to the reaction medium leads to the ionization of mainly 7-4'-3-OH groups of flavonoids, which also leads to a bathochromic shift of the range of Band II. In the case of substitution of 7-OH group, bathochromia is not observed even in the presence of free 4'-3-OH groups [19,33,34].…”

Section: Flavanones (Dihydroflavones) Flavanonols (Dihydroflavonols) ...mentioning

confidence: 96%

Spectrophotometric and Chromatographic Assessment of Total Flavonoids Content in Rhodendron Tomentosum Extracts

Kukhtenko,

Bevz,

Kukhtenko

et al. 2023

Preprint

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In the literature, the chemical composition of Rhododendron tomentosum is mainly represented by the study of isoprenoid compounds of essential oil. In contrast, the study of the content of flavonoids will contribute to the expansion of the pharmacological action and the use of the medicinal plant for medical purposes. The paper deals with the technology of extracts from Rhododendron tomentosum shoots using ethanol of various concentrations and purified water as an extractant. Extracts from Rhododendron tomentosum were obtained by a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material. By the method of high-performance thin-layer chromatography in the system of solvents ethyl acetate: formic acid: water (15:1:1) the following substances have been separated and identified: rutin, hyperoside, quercetin, and chlorogenic acid in all the extracts obtained. To quantify the total flavonoids content, a method of spectrophotometric determination based on the reaction of complexation with aluminum chloride has been developed. It has been proved that the course of the complexation reaction and, accordingly, the quantification of the flavonoid structure substances by the spectrophotometric method is influenced by some factors, including the environment, pH, the structure of the complexes, etc. Regardless of the chosen method, for the further production of Rhododendron tomentosum extracts with a higher content of flavonoid structure substance, it is advisable to use 60%, 70%, and 80% ethyl alcohol as extractant.

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Metabolic Profiling, GC‐MS, LC‐ESI‐MS/MS Analysis, Phenolics Isolation and Biological Evaluation of the Aerial Parts Extracts ofFelicia abyssinicaL.

Elshorbagy,

Fayed,

Sallam

et al. 2024

Chemistry & Biodiversity

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Felicia abyssinica L., family Asteraceae, is widely used in folk medicine. This represents the first study to investigate its phytoconstituents and pharmacological effects. Phytoconstituents identified by GC‐MS, LC‐ESI‐MS/MS‐based metabolomics, and NMR (1D & 2D). GC‐MS of the (FAMEs) revealed mainly the identification of 55 fatty acids. LC‐ESI‐MS/MS resulted in the tentative identity of 13 compounds representing flavonoids, phenolics, and fatty acids. Ethyl acetate fraction exhibited the highest total flavonoids 66.19 mg/mL Rutin equivalent, while the methanolic fraction showed the highest phenolics 87.70 mg/mL gallic acid equivalent, and the total condensed tannins were 64.35 μg CE/mg catechins equivalent. A flavonoid and a cinnamic acid derivative were identified as quercetin 3‐O‐(2′''‐O‐acetyl) rutinoside (Mumikotin A) (1) and Methyl sinapate (2). Biological evaluation of antioxidant and cytotoxic activities was carried out. Cytotoxicity was examined on HepG‐2 cell lines where the average cell viability was 91.42% and 52.48% for concentrations 10 and 100 μg/mL respectively. Methylene chloride and methanolic fractions showed the highest antioxidant activity 225 µg /mL Ascorbic acid equivalents. It is hypothesized that high phenolics, flavonoid content, and oxygenated identified compounds contribute to the antioxidant activity and can be regarded as a promising species for nutraceuticals active antioxidants with potential value for remedy.

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Insight into the Structure Elucidation of Flavonoids ThroughUV-Visible Spectral Analysis of Quercetin Derivatives Using Shift Reagents

Abstract: available with fulltext

Cited by 3 publications

References 0 publications

Flavanones from Erythrina crista-galli Twigs and Their Antioxidant Properties Determined through In Silico and In Vitro Studies

Flavanones from Erythrina crista-galli Twigs and Their Antioxidant Properties Determined through In Silico and In Vitro Studies

Spectrophotometric and Chromatographic Assessment of Total Flavonoids Content in Rhodendron Tomentosum Extracts

Metabolic Profiling, GC‐MS, LC‐ESI‐MS/MS Analysis, Phenolics Isolation and Biological Evaluation of the Aerial Parts Extracts ofFelicia abyssinicaL.

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