Insight into the Unique Fluorescence Quenching Property of Metal-Organic Frameworks upon DNA Binding

Abstract

Metal-organic frameworks (MOFs) have been successfully used as efficient quenchers for fluorescent DNA detection. However, the surface charge property of MOFs can inevitably affect their fluorescence quenching behavior. Herein, nanoscale MOFs (NMOFs), including MOF nanosheets and nanoparticles, have been employed to investigate the relationship between the fluorescence quenching and surface properties of NMOFs. We find that the positively and negatively charged NMOFs exhibited totally opposite fluorescence quenching properties toward negatively charged FAM-labeled double-stranded DNA (dsDNA). On the contrast, they show negligible influence on the sensing of positively charged TAMRA-labeled dsDNA. This study provides a new insight of the fluorescence quenching property of NMOFs and offers a new concept for construction of ratiometric fluorescence DNA biosensors.

Metal-organic frameworks for virus detection

“…The nucleic acid detection is mainly based on the fluorescence quenching/recovery. Compared with the traditional fluorescence nanoquenchers, such as gold nanoparticles ( Li et al, 2018 ; Qian et al, 2020 ; Steinmetz et al, 2019 ), graphene ( Mars et al, 2018 ), graphene oxide (GO) ( Joshi et al, 2020 ; Shin et al, 2020 ), delta-FeOOH nanosheets ( Wu et al, 2020 ), TiO 2 ( Ding et al, 2019 ), and MoS 2 ( Singhal et al, 2018 ), some of which are summarized in Table 1 , MOF possesses a unique fluorescence quenching/recovery with continued decline of fluorescence intensity which was confirmed by wang et al ( Wang et al, 2017 ). ( Fig.…”

“…3 (A) Fluorescence quenching properties of MIL-101 toward FAM-labeled DNA. Reproduced with permission from ( Wang et al, 2017 ). Copyright 2017, American Chemical Society.…”

Recent advances in fluorescence sensors based on DNA–MOF hybrids

“…Metal ions play a significant role and lead to different quenching mechanisms found for carbon nanostructures. Therefore, MOFs can be used as a sensing platform for nucleic acids …”

Two-dimensional nanomaterials for Förster resonance energy transfer–based sensing applications

“…The 2D MOF nanosheets have been widely used in the construction of FRET sensors in nanomedicine as they exhibit several advantages over other 2D nanomaterials such as high structural tunability and degradability [148][149][150][151][152][153]. In the study of Wang et al [149], the two-color intracellular adenosine imaging in living cells was achieved by using the lanthanide-based MOF nanosheets.…”

“…Consequently, the concentration of adenosines in living cells could be monitored in real time. In a later work, the authors further investigated the interaction mechanism of the surface properties of MOFs to the fluorescence quenching of FAM [150]. It was found that the grafted PEI layer on the positively charged MOF surface can block the interaction between FAM and Cr 3+ .…”