Insights into rootstock biology under low oxygen

Abstract

Genetic characterization of lentil (Lens culinaris Medik.) cultivars is important for lentil breeding. Therefore, random amplified polymorphic DNA (RAPD) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis were carried out to evaluate genetic diversity in 13 Turkish lentil cultivars. A total of eight RAPD primers were used in this study; 61 bands were produced and 55 of them were polymorphic (89.78%). The RAPD primers OPA-10, OPB-11, and OPI-13 had the highest polymorphism ratio (100%). As a result of SDS-PAGE analysis, variations in the seed protein pattern were observed among the lentil cultivars being studied. The SDS-PAGE similarity matrices indicated higher genetic similarity estimates among the lentil cultivars than RAPD. In addition, principal components analysis (PCA) was performed for both SDS-PAGE and RAPD where the first three components accounted for 75.760% and 68.121% of the total variation for SDS-PAGE and RAPD analysis, respectively. It was noted that the lentil cultivars with factor loadings greater than 0.5 for each principal component (PC) were also grouped together in the SDS-PAGE and RAPD dendrogram. In addition to genetic diversity, cotyledon color (an important market criterion) values were measured for lightness (L*), redness (a*), and yellowness (b*). As for cotyledon color, values for brightness, redness, and yellowness varied significantly among lentil cultivars. Among the red lentil cultivars, 'Çağıl' and 'Yerli Kırmızı' had the highest cotyledon L* values of 70.83 and 70.74, respectively. The results of both genetic diversity analyses and cotyledon color of lentil cultivars would help in planning future breeding programs to improve high yielding marketable lentil cultivars.

Elevated auxin and reduced cytokinin contents in rootstocks improve their performance and grafting success

“…Traditional breeding efforts have made impressive progress towards improving rootstock performance in numerous plant species, but continued improvement remains limited to selection of existing traits within the gene pool of rootstock cultivars (Cousins, ). Hybridization breeding is also limited, as some elite traits may be lost in the process (van Nocker and Gardiner, ; Pinto, ). Progeny production, through sexual crossing and subsequent selection, is a lengthy and labour‐intensive process that can take a decade to reach fruition.…”

Genetic diversity in several genotypes of Algerian lentil using biochemical markers

“…Kumar et al (2018) reported that band patterns obtained from the 14 lentil cultivars and 23 polypeptide bands were detected and from Sultana and Ghafoor (2008). They recorded 55 protein bands in lentils ranging from the molecular mass of 14 to 66 kDa, and 13 of these were polymorphic, and Erdogan (2015) reported that band patterns were obtained from the 13 lentil cultivars, and a total of 22 polypeptide bands were detected. Therefore, our results are similar to those of the authors (Kumar et al 2018).…”