Insights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells

Périchon, Bruno; Guignot, Julie; Szili, Noémi; Gao, Cherry; Poyart, Claire; Trieu-Cuot, Patrick; Dramsi, Shaynoor

doi:10.1016/j.micinf.2018.10.004

Cited by 13 publications

(11 citation statements)

References 19 publications

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“… a The lowest percent nucleotide (nt) identity of the serotype VIII hit to a VFDB gene among all isolates with that gene b The lowest percent nucleotide (nt) sequence coverage of a serotype VIII gene to the VFDB gene among all isolates with that gene c A variant of the major subunit protein (also called backbone pilin protein) found in some isolates of GBS [ 36 ] d Identified with standalone blastn of PI-2b operon described in [ 31 ] e All isolates had 100% query coverage, except for isolate 10 where the operon was split between two contigs with 42% and 58% query coverage …”

Section: Resultsmentioning

confidence: 99%

“…Virulence factors were identified with abricate v1.0.1 ( https://github.com/tseemann/abricate ) and the core Virulence Factor Database (VFDB) (30-Dec-2021) [ 30 ]. Additional virulence factors were identified using blastn 2.12.0 + of full pilus gene sequences (PI-2b, GCF_000012705.1; PI-1b major subunit allele, WP_000777405.1) [ 31 ] and partial alp/rib genes sequences with a 95% nucleotide identity cutoff to determine gene presence [ 10 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Genomic characterization of emerging invasive Streptococcus agalactiae serotype VIII in Alberta, Canada

Williams

Croxen

Demczuk

et al. 2023

Eur J Clin Microbiol Infect Dis

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Invasive Group B Streptococcus (GBS) can infect pregnant women, neonates, and older adults. Invasive GBS serotype VIII is infrequent in Alberta; however, cases have increased in recent years. Here, genomic analysis was used to characterize fourteen adult invasive serotype VIII isolates from 2009 to 2021. Trends in descriptive clinical data and antimicrobial susceptibility results were evaluated for invasive serotype VIII isolates from Alberta. Isolate genomes were sequenced and subjected to molecular sequence typing, virulence and antimicrobial resistance gene identification, phylogenetic analysis, and pangenome determination. Multilocus sequencing typing identified eight ST42 (Clonal Complex; CC19), four ST1 (CC1), and two ST2 (CC1) profiles. Isolates were susceptible to penicillin, erythromycin, chloramphenicol, and clindamycin, apart from one isolate that displayed erythromycin and inducible clindamycin resistance. All isolates carried genes for peptide antibiotic resistance, three isolates for tetracycline resistance, and one for macrolide, lincosamide, and streptogramin resistance. All genomes carried targets currently being considered for protein-based vaccines (e.g., pili and/or Alpha family proteins). Overall, invasive GBS serotype VIII is emerging in Alberta, primarily due to ST42. Characterization and continued surveillance of serotype VIII will be important for outbreak prevention, informing vaccine development, and contributing to our understanding of the global epidemiology of this rare serotype. Supplementary Information The online version contains supplementary material available at 10.1007/s10096-023-04606-9.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Genomic characterization of emerging invasive Streptococcus agalactiae serotype VIII in Alberta, Canada

Williams

Croxen

Demczuk

et al. 2023

Eur J Clin Microbiol Infect Dis

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“…All GBS strains express one or two pili encoded by three different pilus islands, PI-1, PI-2a, or PI-2b ( 17 ). The GBS COH1 strain used here is a serotype III, sequence type-17 and encodes pilus islands PI-1 and PI-2b, but only PI-2b was shown to be required for interaction with brain endothelium and lung epithelial cells, as well as the development of invasive disease ( 17 , 18 ). To characterize the role of PI-2b in GBS mucosal interactions within the FRT, we confirmed differential expression of genes within the PI-2b locus ( Fig.…”

Section: Observationmentioning

confidence: 99%

Role of MUC5B during Group B Streptococcal Vaginal Colonization

Burcham

Bath

Werlang

et al. 2022

mBio

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Mucin glycoproteins are a major component that contributes to the complexity of the female reproductive tract (FRT). Group B Streptococcus (GBS) is present in the FRT of 25–30% of healthy women, but during pregnancy can ascend to the uterus to cause preterm birth and fetal infection in utero. Here we show that a prominent mucin found in the FRT, MUC5B, promotes host defense by inhibiting GBS interaction with epithelial cells found in the FRT and ascension from the vagina to the uterus in vivo .

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“…The human microbiome is composed of many bacteria, archaea, viruses, and fungi, and is widely distributed in the human oral cavity, respiratory tract, gastrointestinal tract, urogenital tract, and skin [9]. The microbiome is involved in the development of a variety of diseases and is considered the human body's "Second Genome" [10,11]. The homeostasis of the respiratory microbiome is closely related to the immune defense function of the respiratory system.…”

Section: Introductionmentioning

confidence: 99%

Respiratory syncytial virus infection disrupts pulmonary microbiota and immune barriers to induce microglia phenotype shift

Tan

Bajinka

Tang

et al. 2023

Preprint

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Background: The lung-brain-axis is an emerging biological pathway that is being investigated in relation to microbiome medicine. Increasing evidence suggest that pulmonary viral infections can lead to distinct pathological imprints in the brain, thereby the need to explore and understand this mechanism and find possible interventions. Objective: This study used RSV infection in mice as a model to establish the potential lung-brain axis phenomenon. We hypothesized that RSV infection could disrupt the lung microbiota, thereby compromising the immune barriers and thus induces significant shift in microglia phenotype. Methods: Mice were randomized into the Control, Ampicillin, RSV, and RSV+Ampicillin treated groups (n = 6 each). Ampicillin was given intratracheal instillation and seven days after the respective treatments, the mice were anesthetized. Hematoxylin-eosin (HE) staining of lung tissue to detect histopathology. Immunofluorescence label of specific target antigens in both the lung and brain tissues, namely, Malondialdehyde (MDA) and Superoxide dismutase (SOD) were used as markers of cellular damage. RT-qPCR was used to detect viral RNA in both tissues, ELISA to measure IL-1β, iNOS, IL-10 and Arg1 in the supernatant and 16s DNA technology were used to detect the lung microflora. Results: We found out that RSV infection induces elevated oxidative stress, reduced anti-oxidant and caused significant dysbacteriosis in the lungs of the mice. Pulmonary microbes were found affecting Th1-type immunoreactivity induced by RSV infection and eventually, microbiota in lung induced microglia phenotype shift in the brain of the mice. Conclusion. This study was able to establish that RSV infection can disrupt the pulmonary microbiome and immune barriers to induce microglia phenotype shift. Thus, we recommend a large sample size study with robust data analysis for the long-term effects of antibiotics and RSV infection on brain physiology.

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Insights into Streptococcus agalactiae PI-2b pilus biosynthesis and role in adherence to host cells

Cited by 13 publications

References 19 publications

Genomic characterization of emerging invasive Streptococcus agalactiae serotype VIII in Alberta, Canada

Genomic characterization of emerging invasive Streptococcus agalactiae serotype VIII in Alberta, Canada

Role of MUC5B during Group B Streptococcal Vaginal Colonization

Respiratory syncytial virus infection disrupts pulmonary microbiota and immune barriers to induce microglia phenotype shift

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