Insights into the mechanism of the effects of rhizosphere microorganisms on the quality of authentic Angelica sinensis under different soil microenvironments

Abstract

Background Angelica sinensis (Oliv.) Diels (A. sinensis) is a Chinese herb grown in different geographical locations. It contains numerous active components with therapeutic value. Rhizosphere microbiomes affect various aspects of plant performance, such as nutrient acquisition, growth and development and plant diseases resistance. So far, few studies have investigated how the microbiome effects level of active components of A. sinensis. This study investigated whether changes in rhizosphere microbial communities and metabolites of A. sinensis vary with the soil microenvironment. Soils from the two main A. sinensis-producing areas, Gansu and Yunnan Province, were used to conduct pot experiments. The soil samples were divided into two parts, one part was sterilized and the other was unsterilized planting with the seedling variety of Gansu danggui 90–01. All seedlings were allowed to grow for 180 days. At the end of the experiment, radix A. sinensis were collected and used to characterize growth targets and chemical compositions. Rhizosphere soils were subjected to microbial analyses. Results Changes in metabolic profiles and rhizosphere microbial communities of A. sinensis grown under different soil microenvironments were similar. The GN (Gansu non-sterilized), YN (Yunnan non-sterilized), GS (Gansu sterilized), and YS (Yunnan sterilized) groups were significantly separated. Notably, antagonistic bacteria such as Sphingomonas, Pseudomonas, Lysobacter, Pseudoxanthomonas, etc. were significantly (p < 0.05) enriched in Gansu soil compared with Yunnan soil. Moreover, senkyunolide I and ligustilide dimers which were enriched in GS group were strongly positively correlated with Pseudomonas parafulva; organic acids (including chlorogenic acid, dicaffeoylquinic acid and 5-feruloylquinic acid) and their ester coniferyl ferulate which were enriched in YS Group were positively associated with Gemmatimonadetes bacterium WY71 and Mucilaginibater sp., respectively. Conclusions The soil microenvironment influences growth and level/type of active components in A. sinensis. Further studies should explore the functional features of quality-related bacteria, identify the key response genes and clarify the interactions between genes and soil environments. This will reveal the mechanisms that determine the quality formation of genuine A. sinensis.

Long-term continuous mono-cropping of Macadamia integrifolia greatly affects soil physicochemical properties, rhizospheric bacterial diversity, and metabolite contents

“…Actinobacteria, Acidobacteria, and Proteobacteria were the top three abundant bacterial phyla across the three treatment groups. These top three bacterial phyla were also found abundantly in the previous studies conducted in Yunnan province ( Tan et al, 2017 ; Yang et al, 2020 , 2021 ; Kui et al, 2021 ; Zhu et al, 2021 ); however, Proteobacteria was the most dominant bacterial phyla in these studies. The relative abundance of Actinobacteria was first increased from CK to Y5 and then decreased from Y5 to Y20.…”

Explore the interaction between root metabolism and rhizosphere microbiota during the growth of Angelica sinensis

“…Co-occurrence network of the medicinal components and differential microbial taxa in rhizosphere of A. sinensis. Chen et al 10.3389/fpls.2022.1005711 Extensive studies have proved the soil microenvironment influenced the growth and level/type of active components in A. sinensis (Zhu et al, 2021). In this study, we found that the developmental stage is also an important factor affecting the accumulation of medicinal components of A. sinensis.…”

The Deterioration of Agronomical Traits of the Continuous Cropping of Stevia Is Associated With the Dynamics of Soil Bacterial Community

“…These observations indicated that continuous cropping destroyed the balance of original microbial flora and resulted in the imbalance of rhizosphere microecology (Dong et al, 2016 ; Li et al, 2021 ), which would have an important impact on the quality of medicinal plants. Zhu et al ( 2021 ) found that different soil microenvironments significantly affected the growth and active components of Angelica sinensis , and the key bacterial species may be the key factor determining the quality of A. sinensis , which indicated that the soil microenvironment contributed to the growth and accumulation of active components of medicinal plants.…”