Insights into the multi-targeted effects of free nitrous acid on the microalgae Chlorella sorokiniana in wastewater

Abbew, Abdul-Wahab; Qiu, Shuang; Amadu, Ayesha Algade; Qasim, Muhammed Zeeshan; Chen, Zhipeng; Wu, Zhengshuai; Wang, Lingfeng; Ge, Shijian

doi:10.1016/j.biortech.2021.126389

Cited by 34 publications

(6 citation statements)

References 38 publications

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“…Therefore, the decrease of ΦPSII and NPQ suggested that CIP toxicity affected the mechanism of fluorescence quenching by non-photochemical processes. Besides, although PSII reaction center was damaged, induced by impaired PSII-associated electron transport, the synthesis of photosynthetic pigments was still stimulated, which was also observed in C. sorokiniana exposed to free nitrous acid ( Abbew et al., 2021 ).…”

Section: Resultsmentioning

confidence: 92%

“…For example, coproporphyrinogen III oxidase chloroplast precursor, as one of the most important enzymes of chlorophyll synthesis ( Santana et al., 2002 ), accordingly overexpressed 3.47 and 2.33 times in treatment CIP+/Col and CIP++/Col. Delta-aminolevulinic acid dehydratase, which is related to the synthesis of porphobilinogen as one type of chlorophyll-a intermediate ( Abbew et al., 2021 ), was evidently increased 2.25 times in treatment CIP+/Col. Meanwhile, gene related to chlorophyllide a oxygenase activity (gene-C2E21_1445) was found to be upregulated in treatment CIP++/Col.…”

Section: Resultsmentioning

confidence: 99%

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Physiological and transcriptomic responses of Chlorella sorokiniana to ciprofloxacin reveal molecular mechanisms for antibiotic removal

et al. 2022

iScience

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Section: Resultsmentioning

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Physiological and transcriptomic responses of Chlorella sorokiniana to ciprofloxacin reveal molecular mechanisms for antibiotic removal

et al. 2022

iScience

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“…Long-term, a psychrotolerant NOB genus Candidatus Nitrotoga was selected over Nitrospira . However, this occurred after NO 2 - and FNA achieved levels to pose severe toxicity to microalgae (> 25 mg NO 2 -N·L -1 (Aparicio et al, 2021) and >2 mg HNO 2 -N·L -1 FNA (Abbew et al, 2022)).…”

Section: Resultsmentioning

confidence: 99%

Community Structure and Function During Periods of High Performance and System Upset in a Full-Scale Mixed Microalgal Wastewater Resource Recovery Facility

Alam,

Hodaei,

Hartnett

et al. 2024

Preprint

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Microalgae have the potential to exceed current nutrient recovery limits from wastewater, enabling water resource recovery facilities (WRRFs) to achieve increasingly stringent effluent permits. The use of photobioreactors (PBRs) and the separation of hydraulic retention and solids residence time (HRT/SRT) further enables increased biomass in a reduced physical footprint while allowing operational parameters (e.g., SRT) to select for desired functional communities. However, as algal technology transitions to full-scale, there is a need to understand the effect of operational and environmental parameters on complex microbial dynamics among mixotrophic microalgae, bacterial groups, and pests (i.e., grazers and pathogens) and to implement robust process controls for stable long-term performance. Here, we examine the first full-scale, intensive WRRF utilizing mixed microalgal for tertiary treatment in the US (EcoRecover, Clearas Water Recovery Inc.) during a nine-month monitoring campaign. We investigated the temporal variations in microbial community structure (18S and 16S rRNA genes), which revealed that stable system performance of the EcoRecover system was marked by a low-diversity microalgal community (DINVSIMPSON = 2.01) dominated by Scenedesmus sp. (MRA = 55%-80%) that achieved strict nutrient removal (effluent TP < 0.04 mg.L-1) and steady biomass production (TSSmonthly avg. = 400-700 mg.L-1). Operational variables including pH, alkalinity, and influent ammonium (NH4+), correlated positively (p < 0.05, method = Spearman) with algal community during stable performance. Further, the use of these parameters as operational controls along with N/P loading and SRT allowed for system recovery following upset events. Importantly, the presence or absence of bacterial nitrification did not directly impact algal system performance and overall nutrient recovery, but partial nitrification (potentially resulting from NO2- accumulation) inhibited algal growth and should be considered during long-term operation. The microalgal communities were also adversely affected by zooplankton grazers (ciliates, rotifers) and fungal parasites (Aphelidium), particularly during periods of upset when algal cultures were experiencing culture turnover or stress conditions (e.g., nitrogen limitation, elevated temperature). Overall, the active management of system operation in order to maintain healthy algal cultures and high biomass productivity can result in significant periods (>4 months) of stable system performance that achieve robust nutrient recovery, even in winter months in northern latitudes (WI, USA).

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“…Therefore, blue light have been proved to possess more energetic radiation due to its shorter wavelength that ultimately exerts more stressful conditions to Chlorella vulgaris ( C. vulgaris ) cells. 54–56 In the diatom Skeletonema costatum , the photosynthetic quotient (O 2 : CO 2 ) increased along with carbohydrate and lipids on higher irradiance, although proteins were essentially constant over a 10-fold range of irradiances. 52 The growth of Nannochloropsis spp.…”

Section: Comparison Between Different Strategies For Enhancing the Li...mentioning

confidence: 99%

Revisiting algal lipids and cellular stress-causing strategies for ameliorating the productivity of suitable lipids of microalgae for biofuel applications

Parveen

Patidar

2022

Sustainable Energy Fuels

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Insights into the multi-targeted effects of free nitrous acid on the microalgae Chlorella sorokiniana in wastewater

Cited by 34 publications

References 38 publications

Physiological and transcriptomic responses of Chlorella sorokiniana to ciprofloxacin reveal molecular mechanisms for antibiotic removal

Physiological and transcriptomic responses of Chlorella sorokiniana to ciprofloxacin reveal molecular mechanisms for antibiotic removal

Community Structure and Function During Periods of High Performance and System Upset in a Full-Scale Mixed Microalgal Wastewater Resource Recovery Facility

Revisiting algal lipids and cellular stress-causing strategies for ameliorating the productivity of suitable lipids of microalgae for biofuel applications

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