Insights into the Oxidative Stress Alleviation Potential of Enzymatically Prepared Dendrobium officinale Polysaccharides

Abstract

(1) Background: The extraction parameters can dramatically alter the extraction rate and biological activity of polysaccharides. (2) Methods: Here, an enzyme-assisted extraction (EAE) was employed to extract D. officinale polysaccharides (DOPs), and its optimal extraction conditions were established by single-factor and Box–Behnken design (BBD) experiments. Further, on the basis of in vitro antioxidant capacity, the paraquat (PQ)-induced oxidative stress of Caenorhabditis elegans (C. elegans) was chosen as a research model to explore the antioxidant activity of DOPs. (3) Results: The results showed that the extraction yield of DOPs reached 48.66% ± 1.04% under the optimal condition. In vitro experiments had shown that DOPs have considerable ABTS+ radical scavenging capacity (EC50 = 7.27 mg/mL), hydroxyl radical scavenging capacity (EC50 = 1.61 mg/mL), and metal chelating power (EC50 = 8.31 mg/mL). Furthermore, in vivo experiments indicated that DOPs (0.25 mg/mL) significantly prolonged the lifespan, increased antioxidant enzyme activity, and upregulated the expression of daf-16 (>5.6-fold), skn-1 (>5.2-fold), and sir-2.1 (>2.3-fold) of C. elegans. (4) Conclusions: DOPs can be efficiently extracted by EAE and are effective in the reduction of oxidative stress levels in C. elegans.

APPA Increases Lifespan and Stress Resistance via Lipid Metabolism and Insulin/IGF-1 Signal Pathway in Caenorhabditis elegans

“…Based on this, developing drugs or treatments that slow the aging process and boost the body’s antioxidant capacity is crucial. Caenorhabditis elegans is a popular biological model for research on the mechanisms of oxidative stress and aging, due to its ease to culture, short life cycle of 15 to 21 days, strong ability to reproduce, small size, no ethical limitations, numerous mutant strains, low cost, and high productivity [ 5 , 6 , 7 ].…”

Polysaccharides Extracted from Dendrobium officinale Grown in Different Environments Elicit Varying Health Benefits in Caenorhabditis elegans

“…Previous studies on the effect of DOP in C. elegans established a range of effective concentrations [ 38 , 39 ], from which we selected 100, 200 and 500 µg/mL ( Figure 1 , Table S1 ). Our results showed that only GH-DOP significantly extended the lifespan of the worms by 5.9% ( p < 0.01) at 200 µg/mL, and 5.9% ( p < 0.001) at 500 µg/mL compared to the control group ( Figure 1 A–C).…”

Discovery of new triterpene glycosides from Dendrobium officinale with their α-glucosidase and α-amylase inhibitory activity

“…1,2 In Vietnam, D. officinale has been found in the mountains and forests of Vinh Phu, Quang Tri, Quang Nam, Da Nang, Gia Lai, Lam Dong provinces. The previous studies reported terpenoids, 3 bibenzyl derivatives, [1][2][3][4][5][6][7] polysaccharides, [8][9][10][11][12][13][14][15] are the main components of this plants. Some of which exhibited antitumor, 7,14,15 anti-inammatory activities [8][9][10][11][12][13] and alleviates type 2 diabetes mellitus.…”

“…The previous studies reported terpenoids, 3 bibenzyl derivatives, [1][2][3][4][5][6][7] polysaccharides, [8][9][10][11][12][13][14][15] are the main components of this plants. Some of which exhibited antitumor, 7,14,15 anti-inammatory activities [8][9][10][11][12][13] and alleviates type 2 diabetes mellitus. 9 Therefore, the whole plants of D. officinale were selected for further study.…”

“…9 Therefore, the whole plants of D. officinale were selected for further study. This paper reported the isolation, structural identication of thirteen triterpene glycosides (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13), including seven undescribed compounds (1)(2)(3)(4)(5)(6)(7) and their anti a-glucosidase and a-amylase activities in vitro.…”