Insights into the role and structure of plant ureases

Follmer, Cristian

doi:10.1016/j.phytochem.2007.06.034

Cited by 150 publications

(110 citation statements)

References 78 publications

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“…In plants, the primary role of urease is to recycle nutrient nitrogen from arginase-derived urea 14 . Plant ureases are generally homohexamers a 6 with each subunit containing an active site with two Ni 2+ ions 15 . Among them, Canavalia ensiformis (jack bean) urease (JBU) has been the first protein to be crystallized in 1926 16 .…”

Section: Representative Resultsmentioning

confidence: 99%

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Mazzei

Ciurli

Zambelli

2014

JoVE

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Isothermal titration calorimetry (ITC) is a well-described technique that measures the heat released or absorbed during a chemical reaction, using it as an intrinsic probe to characterize virtually every chemical process. Nowadays, this technique is extensively applied to determine thermodynamic parameters of biomolecular binding equilibria. In addition, ITC has been demonstrated to be able of directly measuring kinetics and thermodynamic parameters (k cat , K M , ΔH) of enzymatic reactions, even though this application is still underexploited. As heat changes spontaneously occur during enzymatic catalysis, ITC does not require any modification or labeling of the system under analysis and can be performed in solution. Moreover, the method needs little amount of material. These properties make ITC an invaluable, powerful and unique tool to study enzyme kinetics in several applications, such as, for example, drug discovery.In this work an experimental ITC-based method to quantify kinetics and thermodynamics of enzymatic reactions is thoroughly described. This method is applied to determine k cat and K M of the enzymatic hydrolysis of urea by Canavalia ensiformis (jack bean) urease. Calculation of intrinsic molar enthalpy (ΔH int ) of the reaction is performed. The values thus obtained are consistent with previous data reported in literature, demonstrating the reliability of the methodology. Video LinkThe video component of this article can be found at

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Section: Representative Resultsmentioning

confidence: 99%

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Mazzei

Ciurli

Zambelli

2014

JoVE

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“…Urease also catalyzes the hydrolysis of hydroxyurea, dihydroxyurea, and semicarbazide, with nickel as a co-factor (Alef and Nannipieri, 1995). The enzyme is widely distributed in nature, and it originates from bacteria, yeasts, fungi, algae, animal waste and plants (Follmer, 2008).…”

Section: Characteristics and Role Of Ureasementioning

confidence: 99%

The biological activities of β-glucosidase, phosphatase and urease as soil quality indicators: a review

Adetunji

Lewu

Mulidzi

et al. 2017

J. Soil Sci. Plant Nutr.

369

156

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Soil is a fundamental resource and it is crucial to manage its quality in order to enhance agricultural productivity and environmental quality. Soil enzymes catalyze several biochemical reactions which result in the transformation of organic matter, and the release of inorganic nutrients for plant growth and nutrient cycling. Soil enzyme activities are useful biological soil quality indicators since they are operationally practical, very sensitive, integrative, easy to measure and more responsive to soil tillage and structure than other soil variables. There are several enzymes in soil, but those involved in hydrolases and the degradation of main litter components are used most often for evaluating soil quality. This paper reviews the roles of soil enzymes such as β-glucosidase, phosphatase and urease, as well as the implications of their activities for soil quality.

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“…Bacterial ureases are multimers of two-or three-subunit complexes. The bacterial and plant ureases have high sequence similarity, suggesting that they have similar threedimensional structures and a conserved catalytic mechanism [27,28].…”

Section: Urease Activitymentioning

confidence: 99%

Effective Use of Plant-Derived Urease in the Field of Geoenvironmental/ Geotechnical Engineering

Ran¹,

Kawasaki²

2016

J Civil Environ Eng

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Geotechnical and geo-environmental engineering applications currently being explored through bio mineralization process include cementation of sands to improve mechanical properties and hence, to enhance bearing capacity and liquefaction resistance, sequestration of carbon, soil erosion control via surficial stabilization, groundwater flow control and remediation of soil and groundwater impacted by metals and radionuclides. Bio mediated system broadly refers to a chemical reaction series that are managed and controlled through biological activities and byproducts resulting from those reactions alter the properties of material in the system. Bio mediated methods such as microbial induced calcite precipitation (MICP), biofilm formation, biogas generation and biopolymers have been developed by injecting or stimulating microbes. MICP is the most widely explored method. Microbes having urease activity enhance the hydrolysis of urea and it helps to control the pH and to precipitate carbonate. Although it is a widely explored bio mediated method it has some disadvantages.The plant-derived urease may offer many benefits over microbial urease to induce carbonate cementation. Therefore, it is advantageous to explore the knowledge regarding such a technique as an eco-friendly method for different applications in the fields of geoenvironmental/geotechnical Engineering. The main objective of this paper is to provide an overview of the importance of plant-derived urease for different applications in the fields of geoenvironmental/ geotechnical engineering. The information presented in this paper may be important for biotechnologists/geoengineers to have wide ranging updates on the current situation.

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Insights into the role and structure of plant ureases

Cited by 150 publications

References 78 publications

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

The biological activities of β-glucosidase, phosphatase and urease as soil quality indicators: a review

Effective Use of Plant-Derived Urease in the Field of Geoenvironmental/ Geotechnical Engineering

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Insights into the role and structure of plant ureases

Cited by 150 publications

References 78 publications

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions

The biological activities of &#946;-glucosidase, phosphatase and urease as soil quality indicators: a review

Effective Use of Plant-Derived Urease in the Field of Geoenvironmental/ Geotechnical Engineering

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The biological activities of β-glucosidase, phosphatase and urease as soil quality indicators: a review