Insights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules

Linck, Richard W.; Fu, Xiaofeng; Lin, Jianfeng; Ouch, Christna; Schefter, Alexandra; Walter, Steffen; Warren, Peter; Nicastro, Daniela

doi:10.1074/jbc.m114.568949

Cited by 76 publications

(75 citation statements)

References 90 publications

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“…As K40 is positioned at the luminal face of MTs, it could potentially regulate the interaction with MT inner proteins (MIPs) (Linck et al, 2014) or MT lattice interactions, and thus MT dynamics. Indeed, in C. elegans touch receptor neurons, the organization of the particular 15-protofilament MTs depends on K40 acetylation (Cueva et al, 2012;Topalidou et al, 2012).…”

Section: Molecular Mechanisms Controlled By Tubulin Ptmsmentioning

confidence: 99%

The tubulin code at a glance

Gadadhar

Bodakuntla

Natarajan

et al. 2017

Journal of Cell Science

228

227

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Microtubules are key cytoskeletal elements of all eukaryotic cells and are assembled of evolutionarily conserved α-tubulin-β-tubulin heterodimers. Despite their uniform structure, microtubules fulfill a large diversity of functions. A regulatory mechanism to control the specialization of the microtubule cytoskeleton is the 'tubulin code', which is generated by (i) expression of different α-and β-tubulin isotypes, and by (ii) post-translational modifications of tubulin. In this Cell Science at a Glance article and the accompanying poster, we provide a comprehensive overview of the molecular components of the tubulin code, and discuss the mechanisms by which these components contribute to the generation of functionally specialized microtubules.

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Section: Molecular Mechanisms Controlled By Tubulin Ptmsmentioning

confidence: 99%

The tubulin code at a glance

Gadadhar

Bodakuntla

Natarajan

et al. 2017

Journal of Cell Science

228

227

View full text Add to dashboard Cite

show abstract

“…Labeling techniques that have been combined with cryo-EM imaging include immunogold labeling (15) and, more recently, clonable tags, which have the advantage of higher labeling specificity and resolution (16,17). To avoid disruption of the assembly or architecture of macromolecular complexes, clonable tags are usually designed to be relatively small (e.g.…”

mentioning

confidence: 99%

In Situ Localization of N and C Termini of Subunits of the Flagellar Nexin-Dynein Regulatory Complex (N-DRC) Using SNAP Tag and Cryo-electron Tomography

Song

Awata

Tritschler

et al. 2015

Journal of Biological Chemistry

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Background: Techniques to localize proteins in situ at high resolution are important but limited. Results: Combining SNAP tag technology with cryo-electron tomography, we precisely localized proteins within the N-DRC that are important for ciliary motility. Conclusion: The developed method was applied to localize proteins with ϳ3 nm resolution without interfering with the complex function. Significance: The method is a powerful tool for studies of proteins in situ.

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“…Approximately, 90 % of PCD/KS male patients are affected by AZS and most of them showing dynein genes mutation [16]. It has been demonstrated that tektins participated in the assembly of axonemal microtubules and offered stability to axonemal microtubules [29]. Tektins are a highly conserved family of flagellar and ciliary proteins in many species from invertebrate to higher species, such as Chlamydomonas, sea urchins, zebrafish, hedgehog, rodents, and humans [18,19,28,30,31].…”

Section: Discussionmentioning

confidence: 99%

Association of polymorphisms in tektin-t gene with idiopathic asthenozoospermia in Sichuan, China

Zhang

Ding

et al. 2015

J Assist Reprod Genet

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Purpose The purpose of this research was to study the association between the single nucleotide polymorphisms (SNPs) of the tektin-t gene and idiopathic asthenozoospermia. Methods We conducted sequence analyses of the tektin-t gene in 104 idiopathic asthenozoospermia and 102 fertile men with normospermic parameters in Sichuan, China.Results In this study, we found that allele 136 T (odds ratio [OR] 1.745, 95 % confidence interval [CI] 1.146-2.655, P=0.009) was significantly increased in idiopathic asthenozoospermic patients compared with fertile men. This mutation substitutes a highly conserved arginine at position 46 to cysteine. Moreover, PolyPhen-2 analysis predicted that this variant was Bprobably damaging^. In addition, a novel heterozygous mutation, R207H (c.620G >A), was detected in five asthenozoospermic patients, while there was no detection of this genotype among the fertile candidates, indicating that the mutation was located within a conserved domain predicted by PolyPhen-2 analysis as Bprobably damaging^to the protein.Conclusions These results suggested that tektin-t variants (Arg/Cys+Cys/Cys) were probably one of the high risk genetic factors for idiopathic asthenozoospermia among males in Sichuan, China, while the R207H polymorphism may be associated with idiopathic asthenozoospermia risk.

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Insights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules

Cited by 76 publications

References 90 publications

The tubulin code at a glance

The tubulin code at a glance

In Situ Localization of N and C Termini of Subunits of the Flagellar Nexin-Dynein Regulatory Complex (N-DRC) Using SNAP Tag and Cryo-electron Tomography

Association of polymorphisms in tektin-t gene with idiopathic asthenozoospermia in Sichuan, China

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