Insights into the transcriptome profile of mature biofilm of Salmonella Typhimurium on stainless steels surface

Wang, Huhu; Zhang, Xinxiao; Dong, Yang; Xu, Xinglian

doi:10.1016/j.foodres.2015.08.034

Cited by 22 publications

(20 citation statements)

References 41 publications

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“…The metabolism of bacteria undergoing planktonic growth differs vastly from bacteria in the biofilm-forming state. 3 , 4 One important determinant is the redox state of the microenvironment in which bacteria exist. Sensing of the redox state in the external environment is used by Escherichia coli ( E. coli ) to control the switch between aerobic and anaerobic metabolism using the ArcBA two-component gene regulatory system.…”

Section: Introductionmentioning

confidence: 99%

Redox-active conducting polymers modulate Salmonella biofilm formation by controlling availability of electron acceptors

Gomez-Carretero

Libberton

Rhen

et al. 2017

npj Biofilms Microbiomes

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Biofouling is a major problem caused by bacteria colonizing abiotic surfaces, such as medical devices. Biofilms are formed as the bacterial metabolism adapts to an attached growth state. We studied whether bacterial metabolism, hence biofilm formation, can be modulated in electrochemically active surfaces using the conducting conjugated polymer poly(3,4-ethylenedioxythiophene) (PEDOT). We fabricated composites of PEDOT doped with either heparin, dodecyl benzene sulfonate or chloride, and identified the fabrication parameters so that the electrochemical redox state is the main distinct factor influencing biofilm growth. PEDOT surfaces fitted into a custom-designed culturing device allowed for redox switching in Salmonella cultures, leading to oxidized or reduced electrodes. Similarly large biofilm growth was found on the oxidized anodes and on conventional polyester. In contrast, biofilm was significantly decreased (52–58%) on the reduced cathodes. Quantification of electrochromism in unswitched conducting polymer surfaces revealed a bacteria-driven electrochemical reduction of PEDOT. As a result, unswitched PEDOT acquired an analogous electrochemical state to the externally reduced cathode, explaining the similarly decreased biofilm growth on reduced cathodes and unswitched surfaces. Collectively, our findings reveal two opposing effects affecting biofilm formation. While the oxidized PEDOT anode constitutes a renewable electron sink that promotes biofilm growth, reduction of PEDOT by a power source or by bacteria largely suppresses biofilm formation. Modulating bacterial metabolism using the redox state of electroactive surfaces constitutes an unexplored method with applications spanning from antifouling coatings and microbial fuel cells to the study of the role of bacterial respiration during infection.

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Section: Introductionmentioning

confidence: 99%

Redox-active conducting polymers modulate Salmonella biofilm formation by controlling availability of electron acceptors

Gomez-Carretero

Libberton

Rhen

et al. 2017

npj Biofilms Microbiomes

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“…Genes encoding membrane proteins, cytoplasmic proteins, curli production, transcriptional regulators, cellulose biosynthesis and stress response proteins were all differentially expressed, suggesting a role in the maturation of Salmonella biofilm. Carbon fixation and sulphur metabolism pathways and four novel noncoding small RNAs were observed to be upregulated in mature biofilm, emphasising the interrelationship with more general metabolic processes (Wang et al ., ).…”

Section: Development Of Biofilmsmentioning

confidence: 86%

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

Phillips

2016

Int J of Food Sci Tech

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Biofilms are immobile communities of micro-organisms attached to any surface, such as stainless steel or a food matrix surface or on packaging material. They may be composed of a single species, but more generally, in the natural environment, they consist of mixed species together with an extracellular matrix. Biofilms provide a common mechanism of persistence for a number of bacterial species especially in food processing environments, and therefore, prevention of biofilm formation and the removal of preformed biofilms are an important issue for the food industry. This article reviews the current understanding on the formation of biofilms and recent developments in biological and chemical methods for prevention and removal.

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“…A 0.1 M phosphate buffer solution was added to the TSB to maintain a pH of 6.0 for the acid stress group. Non-attached cells were removed by rinsing the coupons three times with a 0.85% NaCl solution, whereas the attached biofilm cells were detached from the coupons using a violent water-flapping motion that has been proven to effectively remove cells attached to coupons (Wang et al, 2015 ). The coupons were transferred into a stomacher blender bag and shaken at a speed of 200 oscillations/min for 2 min using a bag mixer (BagMixer 400VW, Interscience) to detach the cells from the coupons.…”

Section: Methodsmentioning

confidence: 99%

“…Salmonella can colonize processing surfaces and equipment such as stainless steel, marble and granite, and traditional cleaning and sanitation procedures may not be able to eradicate Salmonella from such surfaces (Rodrigues et al, 2011 ; Arguello et al, 2012 ). Salmonella is usually present on the processing surfaces of food equipment in the form of a biofilm, which can enable cells to cope with harsh conditions, in particular the Typhimurium and Enteritidis serotypes (Simões et al, 2010 ; Finn et al, 2013 ; Wang et al, 2015 ), and cells present in the biofilm matrix are more resistant to routinely used disinfectants than their planktonic cells (Joseph et al, 2001 ; Soni et al, 2013 ). Recent studies have shown that Salmonella species are able to survive under various environmental conditions, being found on poultry, meat, melons and grains as well as in the food processing industry (Bower and Daeschel, 1999 ; Stepanovi et al, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Salmonella Enteritidis Exposure to Acid Stress

et al. 2017

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Salmonella has emerged as a well-recognized food-borne pathogen, with many strains able to form biofilms and thus cause cross-contamination in food processing environments where acid-based disinfectants are widely encountered. In the present study, RNA sequencing was employed to establish complete transcriptome profiles of Salmonella Enteritidis in the forms of planktonic and biofilm-associated cells cultured in Tryptic Soytone Broth (TSB) and acidic TSB (aTSB). The gene expression patterns of S. Enteritidis significantly differed between biofilm-associated and planktonic cells cultivated under the same conditions. The assembled transcriptome of S. Enteritidis in this study contained 5,442 assembled transcripts, including 3,877 differentially expressed genes (DEGs) identified in biofilm and planktonic cells. These DEGs were enriched in terms such as regulation of biological process, metabolic process, macromolecular complex, binding and transferase activity, which may play crucial roles in the biofilm formation of S. Enteritidis cultivated in aTSB. Three significant pathways were observed to be enriched under acidic conditions: bacterial chemotaxis, porphyrin-chlorophyll metabolism and sulfur metabolism. In addition, 15 differentially expressed novel non-coding small RNAs (sRNAs) were identified, and only one was found to be up-regulated in mature biofilms. This preliminary study of the S. Enteritidis transcriptome serves as a basis for future investigations examining the complex network systems that regulate Salmonella biofilm in acidic environments, which provide information on biofilm formation and acid stress interaction that may facilitate the development of novel disinfection procedures in the food processing industry.

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Insights into the transcriptome profile of mature biofilm of Salmonella Typhimurium on stainless steels surface

Cited by 22 publications

References 41 publications

Redox-active conducting polymers modulate Salmonella biofilm formation by controlling availability of electron acceptors

Redox-active conducting polymers modulate Salmonella biofilm formation by controlling availability of electron acceptors

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Salmonella Enteritidis Exposure to Acid Stress

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