Insights of stem cell-based endogenous repair of intervertebral disc degeneration

Yang, Lehe; Li, Yan; Li, Nan; Wang, Zhen; Songyang, Zhou; Feng, Xinmin; Líu, Hao; Zhang, Liang

doi:10.4252/wjsc.v12.i4.266

Cited by 14 publications

(25 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 4 In addition, previous studies showed that stem cells in the adjacent vertebral body could migrate to the NP physiologically through the nutrition channel to maintain the IVD environment balance. 5 , 6 In IVDD, CEP degeneration leads to blockage of the nutrition channel in the endplate, which results in degeneration of the NP and endogenous repair failure. 7 , 8 Therefore, finding an effective method to alleviate CEP degeneration to prevent or reverse IVDD is necessary.…”

Section: Introductionmentioning

confidence: 99%

MSC-Derived Exosomes Protect Vertebral Endplate Chondrocytes against Apoptosis and Calcification via the miR-31-5p/ATF6 Axis

Xie

Chen

Liu

et al. 2020

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

Apoptosis and calcification of endplate chondrocytes (EPCs) can exacerbate intervertebral disc degeneration (IVDD). Mesenchymal stem cell-derived exosomes (MSC-exosomes) are reported to have the therapeutic potential in IVDD. However, the effects and related mechanisms of MSC-exosomes on EPCs are still unclear. We aimed to investigate the role of MSC-exosomes on EPCs with a tert-butyl hydroperoxide (TBHP)-induced oxidative stress cell model and IVDD rat model. First, our study revealed that TBHP could result in apoptosis and calcification of EPCs, and MSC-exosomes could inhibit the detrimental effects. We also found that these protective effects were inhibited after miroRNA (miR)-31-5p levels were downregulated in MSC-exosomes. The target relationship between miR-31-5p and ATF6 was tested. miR-31-5p negatively regulated ATF6-related endoplasmic reticulum (ER) stress and inhibited apoptosis and calcification in EPCs. Our in vivo experiments indicated that sub-endplate injection of MSC-exosomes can ameliorate IVDD; however, after miR-31-5p levels were downregulated in MSC-exosomes, these protective effects were inhibited. In conclusion, MSC-exosomes reduced apoptosis and calcification in EPCs, and the underlying mechanism may be related to miR-31-5p/ATF6/ER stress pathway regulation.

show abstract

Section: Introductionmentioning

confidence: 99%

MSC-Derived Exosomes Protect Vertebral Endplate Chondrocytes against Apoptosis and Calcification via the miR-31-5p/ATF6 Axis

Xie

Chen

Liu

et al. 2020

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

show abstract

“…In recent years, studies on the role of chitosan in cartilage repair are also gradually being carried out (Rusu et al, 2019;George;Tandon and Kandasubramanian, 2020). NP cells are the main components of NP, belonging to the chondroid cells, which can secrete aggrecan and type-II collagen to synthesize extracellular matrix and then maintain the stability of NP (Liu et al, 2020). Theoretically, the gel made of chitosan is also suitable for the nucleus pulposus scaffold of the IVD nucleus pulposus cells.…”

Section: Discussionmentioning

confidence: 99%

The Thermosensitive Injectable Celecoxib-Loaded Chitosan Hydrogel for Repairing Postoperative Intervertebral Disc Defect

Tang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Percutaneous endoscopic lumbar discectomy has been widely used in clinical practice for lumbar spine diseases. But the postoperative disc re-herniation and inflammation are the main reason for pain recurrence after surgery. The postoperative local defect of the intervertebral disc will lead to the instability of the spine, further aggravating the process of intervertebral disc degeneration. In this work, we successfully synthesized the thermosensitive injectable celecoxib-loaded chitosan hydrogel and investigated its material properties, repair effect, biocompatibility, and histocompatibility in in vitro and in vivo study. In vitro and in vivo, the hydrogel has low toxicity, biodegradability, and good biocompatibility. In an animal experiment, this composite hydrogel can effectively fill local tissue defects to maintain the stability of the spine and delay the process of intervertebral disc degeneration after surgery. These results indicated that this composite hydrogel will be a promising way to treat postoperative intervertebral disc disease in future clinical applications.

show abstract

“…The declining number of cells, their phenotype modification and the inability to withhold compression stresses lead to the deterioration of the AF lamellar architecture and the reduction in ECM synthesis [41,42]. The low metabolic activity of cells and the avascular nature of IVD may explain the inability to self-repair following injury and degeneration [43]. However, recent research has reported the presence of progenitor stem cells in the NP, which may be physiologically responsible, to a certain extent, for endogenous repair of intrinsic insults to IVD integrity.…”

Section: Intervertebral Disc Degenerationmentioning

confidence: 99%

Mesenchymal Stem Cell-Derived Exosomes: The New Frontier for the Treatment of Intervertebral Disc Degeneration

et al. 2021

View full text Add to dashboard Cite

Low back pain (LBP) is one of the most frequent symptoms associated with intervertebral disc degeneration (IDD) and affects more than 80% of the population, with strong psychosocial and economic impacts. The main cause of IDD is a reduction in the proteoglycan content within the nucleus pulposus (NP), eventually leading to the loss of disc hydration, microarchitecture, biochemical and mechanical properties. The use of mesenchymal stem cells (MSCs) has recently arisen as a promising therapy for IDD. According to numerous reports, MSCs mediate their regenerative and immunomodulatory effects mainly through paracrine mechanisms. Recent studies have suggested that extracellular vesicles (EVs) extracted from MSCs may be a promising alternative to cell therapy in regenerative medicine. EVs, including exosomes and microvesicles, are secreted by almost all cell types and have a fundamental role in intercellular communication. Early results have demonstrated the therapeutic potential of MSCs-derived EVs for the treatment of IDD through the promotion of tissue regeneration, cell proliferation, reduction in apoptosis and modulation of the inflammatory response. The aim of this review is to focus on the biological properties, function, and regulatory properties of different signaling pathways of MSCs-derived exosomes, highlighting their potential applicability as an alternative cell-free therapy for IDD.

show abstract

Insights of stem cell-based endogenous repair of intervertebral disc degeneration

Cited by 14 publications

References 69 publications

MSC-Derived Exosomes Protect Vertebral Endplate Chondrocytes against Apoptosis and Calcification via the miR-31-5p/ATF6 Axis

MSC-Derived Exosomes Protect Vertebral Endplate Chondrocytes against Apoptosis and Calcification via the miR-31-5p/ATF6 Axis

The Thermosensitive Injectable Celecoxib-Loaded Chitosan Hydrogel for Repairing Postoperative Intervertebral Disc Defect

Mesenchymal Stem Cell-Derived Exosomes: The New Frontier for the Treatment of Intervertebral Disc Degeneration

Contact Info

Product

Resources

About