Insights on the Quantitative Concurrent Fluorescence-Based Analysis of Anti-COVID-19 Drugs Remdesivir and Favipiravir

Abstract

We hereby introduce a sensitive fast straightforward spectrofluorometric method for the estimation of remdesivir and favipiravir. The two drugs are prescribed in some regimens to treat COVID‐19 pandemic disease, which is caused by SARS‐CoV‐2. The method is based on the first derivative synchronous spectrofluorimetry approach for the measurement of remdesivir and favipiravir. This was accomplished at 251 nm and 335 nm respectively using the first derivative order at delta lambda of 140 nm. A linear response with a correlation coefficient 0.9994 was achieved between the concentration and the derivative amplitudes in the ranges of 20.0–100.0 ng ml−1 and 40.0–100.0 ng ml−1 for remdesivir and favipiravir, respectively. The methods were validated for different parameters as stated by the pharmacopeial rules and were applied successfully for estimation of the studied drugs in their synthetic mixtures and in spiked human plasma samples. No significant difference was observed between the proposed and comparison methods as revealed from the analysis of data.

Recent analytical methodologies for the determination of anti-covid-19 drug therapies in various matrices: a critical review

“…Ecofriendly solvents were used during all procedures, which adds greenness to the numerous advantages of this method. 56…”

“…Ecofriendly solvents were used during all procedures, which adds greenness to the numerous advantages of this method. 56 Attia et al developed a sensitive, simple TLC method and applied it for the simultaneous estimation of RMD and FVP in dosage forms and human plasma using TLC plates without any signicant interference. Samples were spotted on normal phase TLC plates, then developed using a mobile phase composed of ethyl acetate-methanol-ammonia and visualized at 235 nm.…”

Synergistic effect of gold nanoparticles anchored on conductive carbon black as an efficient electrochemical sensor for sensitive detection of anti-COVID-19 drug Favipiravir in absence and presence of co-administered drug Paracetamol

“…Favipiravir can be determined by a variety of analytical methods. Spectrofluorometry [4] , [5] , high-performance liquid chromatography (HPLC) [6] , fluorescence [7] , TLC [8] and liquid chromatography-tandem mass spectrometry (LC–MS/MS) [9] have been utilized. Most of these techniques may require expensive equipment, long analysis time, tedious sample preparation, and technical expertise.…”

Fluorimetric Determination of Antiviral (COVID-19) Drug Favipiravir in Bulk and Pharmaceutical Dosage Forms

“…Comparative studies of method developed for analyzing Favipiravir in Pharmaceutical preparations using HPLC and UV were also found 15 . Concurrent Fluorescence based analysis of remdesivir and favipiravir 16 , Spectrofluorimetric analysis of Favipiravir, remdesivir and hydroxychloroquine spiked in human plasma 17 are the two methods found for analysis of Favipiravir along with other drugs using Fluorescence. Since there were no reported methods found for individual analysis of Favipiravir using fluorescence, the present method was developed.…”