Inspiration from heart development: Biomimetic development of functional human cardiac organoids

Abstract

Recent progress in human organoids has provided 3D tissue systems to model human development, diseases, as well as develop cell delivery systems for regenerative therapies. While direct differentiation of human embryoid bodies holds great promise for cardiac organoid production, intramyocardial cell organization during heart development provides biological foundation to fabricate human cardiac organoids with defined cell types. Inspired by the intramyocardial organization events in coronary vasculogenesis, where a diverse, yet defined, mixture of cardiac cell types self-organizes into functional myocardium in the absence of blood flow, we have developed a defined method to produce scaffold-free human cardiac organoids that structurally and functionally resembled the lumenized vascular network in the developing myocardium, supported hiPSC-CM development and possessed fundamental cardiac tissue-level functions. In particular, this development-driven strategy offers a robust, tunable system to examine the contributions of individual cell types, matrix materials and additional factors for developmental insight, biomimetic matrix composition to advance biomaterial design, tissue/organ-level drug screening, and cell therapy for heart repair.

A review of biomaterials in bone defect healing, remaining shortcomings and future opportunities for bone tissue engineering

“…New strategies for enhanced biomaterials include bioinspired composite matrices, 94 biogenic hydroxyapatites 95 or biomimetic organoids, 96 . These indicate the development towards biomaterials that copy the naturally occurring tissues/conditions through our advanced understanding of the physiological mechanisms coupled with the highly developed techniques available today.…”

Capturing Neonatal Cardiomyocyte – Endothelial interactions on-a-Chip

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George Lucas : Prophète du transhumanisme ?

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