Instant trachea reconstruction using 3D-bioprinted C-shape biomimetic trachea based on tissue-specific matrix hydrogels

Sun, Yuyan; Huo, Yingying; Ran, Xinyue; Chen, Hongying; Pan, Qingqing; Chen, Yujie; Zhang, Ying; Ren, Wenjie; Wang, Xiaoyun; Zhou, Guangdong; Hua, Yujie

doi:10.1016/j.bioactmat.2023.09.011

Cited by 8 publications

(1 citation statement)

References 48 publications

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“…Cytocompatibility testing of the GC-Gel and GE-Gel showed no cytotoxicity reinforcing the photocrosslinkable nature of these hydrogels. A 19-min print time eases any trouble of inefficiency with the printing having no impact on cell viability [57]. Huo et al expanded on this concept by placing the 3DP cartilage vascularised fibrous tissue integrated trachea in a rabbit model wrapped by a vascular muscle flap.…”

Section: Bioink Usage For Structural Biomimickingmentioning

confidence: 99%

Surgical Innovations in Tracheal Reconstruction: A Review on Synthetic Material Fabrication

Khalid,

Uchikov,

Hristov

et al. 2023

Medicina

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Background and Objectives: The aim of this review is to explore the recent surgical innovations in tracheal reconstruction by evaluating the uses of synthetic material fabrication when dealing with tracheomalacia or stenotic pathologies, then discussing the challenges holding back these innovations. Materials and Methods: A targeted non-systematic review of published literature relating to tracheal reconstruction was performed within the PubMed database to help identify how synthetic materials are utilised to innovate tracheal reconstruction. Results: The advancements in 3D printing to aid synthetic material fabrication have unveiled promising alternatives to conventional approaches. Achieving successful tracheal reconstruction through this technology demands that the 3D models exhibit biocompatibility with neighbouring tracheal elements by encompassing vasculature, chondral foundation, and immunocompatibility. Tracheal reconstruction has employed grafts and scaffolds, showing a promising beginning in vivo. Concurrently, the integration of resorbable models and stem cell therapy serves to underscore their viability and application in the context of tracheal pathologies. Despite this, certain barriers hinder its advancement in surgery. The intricate tracheal structure has posed a challenge for researchers seeking novel approaches to support its growth and regeneration. Conclusions: The potential of synthetic material fabrication has shown promising outcomes in initial studies involving smaller animals. Yet, to fully realise the applicability of these innovative developments, research must progress toward clinical trials. These trials would ascertain the anatomical and physiological effects on the human body, enabling a thorough evaluation of post-operative outcomes and any potential complications linked to the materials or cells implanted in the trachea.

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Section: Bioink Usage For Structural Biomimickingmentioning

confidence: 99%

Surgical Innovations in Tracheal Reconstruction: A Review on Synthetic Material Fabrication

Khalid,

Uchikov,

Hristov

et al. 2023

Medicina

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Multi‐Tissue Integrated Tissue‐Engineered Trachea Regeneration Based on 3D Printed Bioelastomer Scaffolds

Song,

Zhang,

Luo

et al. 2024

Advanced Science

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Functional segmental trachea reconstruction is a critical concern in thoracic surgery, and tissue‐engineered trachea (TET) holds promise as a potential solution. However, current TET falls short in fully restoring physiological function due to the lack of the intricate multi‐tissue structure found in natural trachea. In this research, a multi‐tissue integrated tissue‐engineered trachea (MI‐TET) is successfully developed by orderly assembling various cells (chondrocytes, fibroblasts and epithelial cells) on 3D‐printed PGS bioelastomer scaffolds. The MI‐TET closely resembles the complex structures of natural trachea and achieves the integrated regeneration of four essential tracheal components: C‐shaped cartilage ring, O‐shaped vascularized fiber ring, axial fiber bundle, and airway epithelium. Overall, the MI‐TET demonstrates highly similar multi‐tissue structures and physiological functions to natural trachea, showing promise for future clinical advancements in functional TETs.

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Scaffold‐free three‐dimensional cartilage regeneration based on cartilaginous organoids bioassembly technology

Huo,

Ci,

et al. 2024

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Cartilage tissue engineering is a promising strategy to repair damaged tissue and reconstruct organ function, but the scaffold‐free cartilage regeneration technology is currently limited in its ability to construct three‐dimensional (3D) shapes, maintain the chondrogenic phenotype, and express cartilage‐specific extracellular matrix (ECM). Recently, cartilaginous organoids (COs), multicellular aggregates with spheroid architecture, have shown great potential in miniaturized cartilage developmental models in vitro. However, high‐efficiency and transferable in vivo organoid‐based 3D cartilage regeneration technology for preclinical research needs further exploration. In this study, we develop novel cartilaginous organoids bioassembly (COBA) strategy to achieve scaffold‐free 3D cartilage regeneration, which displays batch‐to‐batch efficiency, structural integration, and functional reconstruction. For underlying molecule mechanism, cellular adhesion proteins significantly regulate cell aggregation and cytoskeleton reorganization to form cartilaginous spheroids, and the hypoxic microenvironment created by high‐density cell aggregates synergistically activates hypoxia‐inducible factor‐1α‐mediated glycolytic metabolism reprogramming to maintain the chondrogenic phenotype and promote cartilage‐specific ECM deposition. Furthermore, separated COs can integrate into a complete and continuous cartilage tissue through the COBA approach, and thus facilitate raising the nasal dorsa in goats after minimally invasive injection. This study thus demonstrates the promise of COBA technology to achieve scaffold‐free 3D cartilage regeneration for organoid‐based translational applications.

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Instant trachea reconstruction using 3D-bioprinted C-shape biomimetic trachea based on tissue-specific matrix hydrogels

Cited by 8 publications

References 48 publications

Surgical Innovations in Tracheal Reconstruction: A Review on Synthetic Material Fabrication

Surgical Innovations in Tracheal Reconstruction: A Review on Synthetic Material Fabrication

Multi‐Tissue Integrated Tissue‐Engineered Trachea Regeneration Based on 3D Printed Bioelastomer Scaffolds

Scaffold‐free three‐dimensional cartilage regeneration based on cartilaginous organoids bioassembly technology

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