Instrumentation and software for mass spectrometry imaging—Making the most of what you've got

Trim, Paul J.; Djidja, Marie Claude; Muharib, Tasneem; Cole, Laura; Flinders, Bryn; Carolan, Vikki A.; Francese, Simona; Clench, Malcolm R.

doi:10.1016/j.jprot.2012.07.016

Cited by 20 publications

(13 citation statements)

References 33 publications

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“…A number of techniques have been developed to drive the mass spectrometers to perform MSI, with MALDI, desorption electrospray ionisation (DESI) or laser ablation electrospray ionisation (LAESI) as the means of generating ions [55,56]. The UltrafleXtreme™ (Bruker Daltonics) MALDI-TOF-TOF-MS platform is an example of a useful configuration for MSI [29].…”

Section: Methodological Advances In Sample Preparationmentioning

confidence: 99%

Current Status and Future Perspectives of Mass Spectrometry Imaging

Nimesh

Mohottalage

Vincent

et al. 2013

IJMS

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Mass spectrometry imaging is employed for mapping proteins, lipids and metabolites in biological tissues in a morphological context. Although initially developed as a tool for biomarker discovery by imaging the distribution of protein/peptide in tissue sections, the high sensitivity and molecular specificity of this technique have enabled its application to biomolecules, other than proteins, even in cells, latent finger prints and whole organisms. Relatively simple, with no requirement for labelling, homogenization, extraction or reconstitution, the technique has found a variety of applications in molecular biology, pathology, pharmacology and toxicology. By discriminating the spatial distribution of biomolecules in serial sections of tissues, biomarkers of lesions and the biological responses to stressors or diseases can be better understood in the context of structure and function. In this review, we have discussed the advances in the different aspects of mass spectrometry imaging processes, application towards different disciplines and relevance to the field of toxicology.

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Section: Methodological Advances In Sample Preparationmentioning

confidence: 99%

Current Status and Future Perspectives of Mass Spectrometry Imaging

Nimesh

Mohottalage

Vincent

et al. 2013

IJMS

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“…Recent advancements in the field of IMS have specifically been reviewed and discussed showing the great potential of this technique in small molecule analysis [61,62]. Through a computer-controlled xy stage to the ionization source, the surface of the sample is typically scanned with a local desorbing and ionizing probe, and the generated ions from the surface are analyzed by MS [63]. In are the object of an imaging study.…”

Section: Imaging Msmentioning

confidence: 99%

Direct Mass Spectrometry-Based Approaches in Metabolomics

Ibáñez

García‐Cañas

Valdés

et al. 2014

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites

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Metabolomics is one of the newest omics technologies concerned with the identification and quantification of small molecules in a high-throughput manner. Considering the number of different types of metabolites present in a wide dynamic range of concentrations in any single living system, still actual analytical technologies can only capture a part of the metabolome.Currently MS-based approaches yield a higher sensitivity than NMR when analyzing minimal amounts of complex mixtures. Most MS-based approaches in metabolomics involve a physical/chemical purification/fractionation prior to MS analysis, to avoid sample matrix effects, at the expenses of low high-throughput performance. In the quest to achieve the maximum highthroughput production of metabolite information in the largest possible number of samples an extensive array of direct ionization or desorption/ionization techniques have been developed and combined. In the present Chapter an overview of the main desorption/ionization techniques coupled to MS applied to direct metabolite profiling or fingerprinting is presented.

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“…Other applications of SIMS MSI for the analysis of biological samples are thoroughly reviewed elsewhere (Lanni et al, 2012). For further reading on the strengths, weaknesses, and complementarity of existing MSI methods, we recommend two recent comprehensive reviews (Vickerman, 2011;Trim et al, 2012).…”

Section: Chemical Maps With Mass Spectrometry Imagingmentioning

confidence: 99%

Small-Volume Analysis of Cell–Cell Signaling Molecules in the Brain

Romanova

Aerts

Croushore

et al. 2013

Neuropsychopharmacol

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Modern science is characterized by integration and synergy between research fields. Accordingly, as technological advances allow new and more ambitious quests in scientific inquiry, numerous analytical and engineering techniques have become useful tools in biological research. The focus of this review is on cutting edge technologies that aid direct measurement of bioactive compounds in the nervous system to facilitate fundamental research, diagnostics, and drug discovery. We discuss challenges associated with measurement of cell-to-cell signaling molecules in the nervous system, and advocate for a decrease of sample volumes to the nanoliter volume regimen for improved analysis outcomes. We highlight effective approaches for the collection, separation, and detection of such small-volume samples, present strategies for targeted and discovery-oriented research, and describe the required technology advances that will empower future translational science.

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Instrumentation and software for mass spectrometry imaging—Making the most of what you've got

Cited by 20 publications

References 33 publications

Current Status and Future Perspectives of Mass Spectrometry Imaging

Current Status and Future Perspectives of Mass Spectrometry Imaging

Direct Mass Spectrometry-Based Approaches in Metabolomics

Small-Volume Analysis of Cell–Cell Signaling Molecules in the Brain

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