Instrumentation for Small-Bore Liquid Chromatography

Fundamental Relationships of Chromatography

“…The concentration at the peak maximum, C mx , is given by (8η/ττ ) 1/2 /e T Ld c 2(l + k) (1.75) where m is the mass of injected sample [193,194,[239][240][241][242]. ) The concentration at the peak maximum, C mx , is given by (8η/ττ ) 1/2 /e T Ld c 2(l + k) (1.75) where m is the mass of injected sample [193,194,[239][240][241][242]. )…”

“…The standard deviation of a peak eluting from a column is given by a col = V m (l + k)/n 1/2 (1.76) where σ is the standard deviation of the eluted peak in volume units (peak volume is equivalent to 4 0" col ) and V m is the column holdup volume. If a 10% loss in column efficiency due to extracolumn dispersion is considered acceptable then the peak standard deviation due to extracolumn contributions should not exceed approximately 0.32 times the standard deviation of the peak volume [193,207,[241][242][243][244][245][246]. If a 10% loss in column efficiency due to extracolumn dispersion is considered acceptable then the peak standard deviation due to extracolumn contributions should not exceed approximately 0.32 times the standard deviation of the peak volume [193,207,[241][242][243][244][245][246].…”