Instrumentation for Vibrational Circular Dichroism Spectroscopy: Method Comparison and Newer Developments

Keiderling, Timothy A.

doi:10.3390/molecules23092404

Cited by 27 publications

(23 citation statements)

References 162 publications

(282 reference statements)

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“…Vibrational circular dichroism (VCD) may be considered for directly unveiling the chirality of chitin nanocrystals in aqueous medium. This technique has been proven to work in solution or gel states 49 and has been useful to identify peptide and protein fiber chiral features and to distinguish related variation in conformations. 50 Noting that interactions with water significantly alter the spectra and that a sufficiently high concentration is needed for VCD analysis, its application to chitin nanocrystals needs to be evaluated.…”

Section: Resultsmentioning

confidence: 99%

Chirality from Cryo-Electron Tomograms of Nanocrystals Obtained by Lateral Disassembly and Surface Etching of Never-Dried Chitin

et al. 2020

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The complex nature of typical colloids and corresponding interparticle interactions pose a challenge in understanding their self-assembly. This specially applies to biological nanoparticles, such as those obtained from chitin, which typically are hierarchical and multidimensional. In this study, we obtain chitin nanocrystals by one-step heterogeneous acid hydrolysis of never-dried crab residues. Partial deacetylation facilitates control over the balance of electrostatic charges (zeta potential in the range between +58 and +75 mV), and therefore

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Section: Resultsmentioning

confidence: 99%

Chirality from Cryo-Electron Tomograms of Nanocrystals Obtained by Lateral Disassembly and Surface Etching of Never-Dried Chitin

et al. 2020

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“…A technique that has surely not yet developed all its potential is vibrational circular dichroism (VCD). , This is a technique offering interesting possibilities as the theoretical modeling of the experimental spectra is simpler than for UV–vis spectra. New instrumentation is being developed to increase the instrument sensitivity for weak dichroic signals, but it is mostly optimized for specific problems, for example, for chiral studies on biopolymers …”

Section: Methods For Chirality Determinationmentioning

confidence: 99%

Spontaneous Deracemizations

et al. 2021

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Spontaneous deracemizations is a challenging, multidisciplinary subject in current chirality research. In the absence of any chiral inductors, an achiral substance or a racemic composition is driven into an enantioenriched or even homochiral state through a selective energy input, e.g., chemical potential, photoirradiation, mechanical grinding, ultrasound waves, thermal gradients, etc. The most prominent examples of such transformations are the Soai reaction and the Viedma deracemization. In this review, we track the most recent developments in this topic and recall that many other deracemizations have been reported for solutions from mesophases to conglomerate crystallizations. A compiled set of simply available achiral organic, inorganic, organometallic, and MOF compounds, yielding conglomerate crystals, should give the impetus to realize new experiments on spontaneous deracemizations. Taking into account thermodynamic constraints, modeling efforts have shown that structural features alone are not sufficient to describe spontaneous deracemizations. As a guideline of this review, particular attention is paid to the physicochemical origin and symmetry requirements of such processes.

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“…Consequently, one can scan relatively quickly over the band and coadd multiple spectra to improve S/N, without spending measurement time or acquiring noise from spectral regions of no interest. These instrument designs have been reviewed and compared in some detail in the past. ,,,,,,,,,,− …”

Section: Instrumentation Overviewmentioning

confidence: 99%

“…In particular, conference proceedings and meeting abstracts are not referenced. However, there are a great many previous reviews in this field, which tend toward a more general nature and provide added background (refs , , , , − , , , − , − , − ). Those that emphasize biopolymer studies with VCD or ROA will be referenced as appropriate to enable the reader to access older work in more detail, leaving this review to emphasize more focused developments.…”

Section: Introductionmentioning

confidence: 99%

Structure of Condensed Phase Peptides: Insights from Vibrational Circular Dichroism and Raman Optical Activity Techniques

2020

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Peptides and proteins are naturally chiral molecular systems so that sensing their structure and conformation with chirality-based spectral methods is an obvious and long-used diagnostic application. Extending chiroptical techniques to measurement of vibrational transitions, in the form of vibrational circular dichroism (VCD) and Raman optical activity (ROA), expands the number and types of excitations available that might provide structural insight and can provide an alternate and, in some cases, a more distinctive conformational probe. Since the dominant repeating structural element in peptides is the locally achiral amide group, VCD senses the polymeric structure through amide coupling, which is directly dependent on secondary structure. Determination of the type and relative contribution of these structural components through empirical correlation with spectral character has been the main application of VCD for peptides and proteins, although this is now reinforced by extensive theoretical modeling. Monitoring structural and conformational change induced by environmental perturbations provides another important application. More recently, VCD has been used to detect morphological variations in fibril states of aggregated peptides and proteins. ROA has parallel secondary structural sensitivities, with more applications for proteins than peptides, and has more sensitivity to local configuration and side chains. This review covers the range of peptide studies done with VCD and extends them to compare with example protein and ROA applications.

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Instrumentation for Vibrational Circular Dichroism Spectroscopy: Method Comparison and Newer Developments

Cited by 27 publications

References 162 publications

Chirality from Cryo-Electron Tomograms of Nanocrystals Obtained by Lateral Disassembly and Surface Etching of Never-Dried Chitin

Chirality from Cryo-Electron Tomograms of Nanocrystals Obtained by Lateral Disassembly and Surface Etching of Never-Dried Chitin

Spontaneous Deracemizations

Structure of Condensed Phase Peptides: Insights from Vibrational Circular Dichroism and Raman Optical Activity Techniques

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