Insulin-Like Growth Factor-1 and Selected Insulin-Like Growth Factor Binding Protein Concentrations during an Ultramarathon Sled Dog Race

Brunke, Matthew W.; Frye, Christopher W.; Levine, Corri B.; Hansen, Cristina M.; Wakshlag, Joseph J.

doi:10.1155/2016/5686372

Cited by 2 publications

(1 citation statement)

References 23 publications

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“…IGFBP-blocked double-antibody sandwich ELISA was used for IGF-I and IGF-II. The IGF-I and IGF-II ELISA kits have been validated previously for use in dogs [ 17 18 19 ]. Briefly, the standards at different concentrations and serum samples were reconstituted with an acid buffer to dissociate and block IGFBP.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of circulating IGF-I and IGFBP-3 as biomarkers for tumors in dogs

Song

Sur

et al. 2021

J Vet Sci

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Background Serum-based parameters are considered non-invasive biomarkers for cancer detection. In human studies, insulin-like growth factor-I and II (IGF-I and IGF-II) and insulin-like growth factor binding protein-3 (IGFBP-3) are useful as diagnostic or prognostic markers and potential therapeutic targets. Objectives This study examined the diagnostic utility of circulating IGF-I, IGF-II, and IGFBP-3 levels in healthy dogs and dogs with tumors. Methods The serum concentrations of these biomarkers in 86 dogs with tumors were compared with those in 30 healthy dogs using an enzyme-linked immunosorbent assay (ELISA). Results The ELISA results showed no difference between healthy dogs and dogs with tumors in the serum IGF-II concentrations. On the other hand, there was a significant difference in the circulating IGF-I and IGFBP-3 levels between healthy dogs and dogs with tumors. The concentrations of serum IGF-I (median [interquartile range], 103.4 [59.5–175] ng/mL) in dogs with epithelial tumors were higher than those (58.4 ng/mL [43.5–79.9]) in healthy dogs. Thus, the concentrations of serum IGFBP-3 (43.4 ng/mL [33.2–57.2]) in dogs with malignant mesenchymal tumors were lower than those (60.8 ng/mL [47.6–70.5]) in healthy dogs. Conclusions The serum IGF-I and IGFBP-3 levels can be used as diagnostic biomarkers in dogs with tumors.

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Section: Methodsmentioning

confidence: 99%

Evaluation of circulating IGF-I and IGFBP-3 as biomarkers for tumors in dogs

Song

Sur

et al. 2021

J Vet Sci

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Serum myostatin decreases in exercising and aging Alaskan sled dogs, while growth and differentiation factor 15 remains unaltered

Shiomitsu

Hansen

Lenfest

et al. 2022

javma

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OBJECTIVES To evaluate the serum concentrations of myostatin and growth and differentiation factor 15 (GDF-15) in Alaskan Husky sled dogs participating in a 350-mile (560-km) race and in an older population, and to examine correlations between changes in serum concentrations and body condition scores (BCSs). ANIMALS Dogs were recruited from 3 teams of Alaskan Huskies participating in the Alaskan–Yukon Quest sled-dog race and retirees from a research sled-dog colony. PROCEDURES Serum samples and BCSs were collected prior to racing, midway, and postrace; and in an older cohort (13 to 14 years). Myostatin and GDF-15 concentrations were assessed using commercially available ELISA kits. RESULTS The median myostatin prerace concentration (9,519 pg/mL) was significantly greater than the mid- and postrace concentrations (7,709 pg/mL and 3,247 pg/mL, respectively). The prerace concentration was also significantly greater than that of the retired sled group dogs at 6,134 pg/mL. GDF-15 median serum concentrations did not change significantly across any racing time point (approx 350 pg/mL) or in the older cohort. No significant correlations were observed between changes in BCS and myostatin or GDF-15 concentrations. CLINICAL RELEVANCE Serum myostatin decreases dramatically, yet no correlations to loss of BCS could be found. Myostatin signaling may be involved in maintaining hypertrophic signaling during intense exercise. Neither racing distance nor geriatric/retirement status appears to have an effect on serum GDF-15 concentration. Myostatin was less in the older, retired sled dogs compared to the younger racing cohort. Such differences highlight the roles that fitness level and age play regarding myostatin levels.

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Insulin-Like Growth Factor-1 and Selected Insulin-Like Growth Factor Binding Protein Concentrations during an Ultramarathon Sled Dog Race

Cited by 2 publications

References 23 publications

Evaluation of circulating IGF-I and IGFBP-3 as biomarkers for tumors in dogs

Evaluation of circulating IGF-I and IGFBP-3 as biomarkers for tumors in dogs

Serum myostatin decreases in exercising and aging Alaskan sled dogs, while growth and differentiation factor 15 remains unaltered

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