Insulin-like growth factor (IGF)-II and insulin, but not IGF-I, are mitogenic for fetal rat adrenal cells in vitro

Dijk, J.P. van; Tanswell, A. Keith; Challis, John

doi:10.1677/joe.0.1190509

Cited by 28 publications

(5 citation statements)

References 58 publications

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“…One of the candidates for local adrenocortical autocrine or paracrine factors is insulin-like growth factors (IGFs). They have been reported to be mitogens for adreno¬ cortical cells in vitro (Horiba et al 1987;Van Dijk et al 1988;Naaman et al 1989). ACTH has been found to increase IGF-II mRNA expression in human fetal adrenocortical cells (Voutilainen & Miller, 1987), and IGF-I peptide synthesis in bovine adrenocortical cells (Penhoat et al 1989).…”

Section: Discussionmentioning

confidence: 99%

Role of adenylate cyclase-cyclic AMP-dependent signal transduction in the ACTH-induced biphasic growth effect of rat adrenocortical cells in primary culture

Arola

Heikkilä²,

Voutilainen³

et al. 1993

Journal of Endocrinology

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ACTH has a biphasic effect on the proliferation of fetal rat adrenocortical cells in primary culture. Dramatic changes occurred during the first 72 h of ACTH stimulation, when incorporation of bromodeoxyuridine was used as an indicator of proliferation. The primary effect of ACTH was the inhibition of proliferation during the first 24 h, which was followed by an intense stimulatory phase during the third day of ACTH treatment. Cycloheximide (a protein synthesis inhibitor) prevented both the inhibitory and the stimulatory effects of ACTH, but did not affect the basal proliferation of unstimulated zona glomerulosa-like cells. Although adrenocortical cells stimulated with cyclic AMP (cAMP) derivatives, 8-bromo cAMP (8-Br cAMP) or dibutyryl cAMP ((Bu)2cAMP), differentiated morphologically into fasciculata-like cells, and secreted corticosterone and 18-OH-deoxycorticosterone, as did ACTH-stimulated cells, neither of the derivatives inhibited proliferation during the first 24 h of treatment. In contrast to ACTH, (Bu)2cAMP had a stimulatory effect on bromodeoxyuridine incorporation during the first 24 h of treatment. 8-Br cAMP did not change proliferation during the 24 h of treatment, but had a stimulatory effect after 72 h, which was not seen with (Bu)2cAMP. Thus, these results suggest that (1) differentiation, steroid hormone synthesis and the mitogenic effect of ACTH are transduced through the cAMP-mediated system, (2) the antimitogenic effect of ACTH is transduced via a cAMP-independent pathway and (3) both antimitogenic and mitogenic effects of ACTH are dependent on protein synthesis.

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Section: Discussionmentioning

confidence: 99%

Role of adenylate cyclase-cyclic AMP-dependent signal transduction in the ACTH-induced biphasic growth effect of rat adrenocortical cells in primary culture

Arola

Heikkilä²,

Voutilainen³

et al. 1993

Journal of Endocrinology

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“…ACTH may act directly as a trophic factor, indirectly via the adrenal glucocorticoids or by stimulation of a local or paracrine growth factor such as fibroblast growth factor (FGF) or the in sulin-like growth factors (IG Fs). IGF I and IGF II mRNA's have been localized in the adrenal cortex of the human fetus [27] and in the presence of insulin, IGF II has been shown to stimulate mitogenesis of fetal rat adrenocortical cells [28,29]. ACTH enhances the expression of FGF in the human fetal adrenal and in vitro studies have shown that FGF stimulates the growth of adrenocortical cells [28,30].…”

Section: Discussionmentioning

confidence: 99%

Different Roles for the Pituitary and Adrenal Cortex in the Control of Enkephalin Peptide Localization and Cortico-Medullary Interaction in the Sheep Adrenal during Development

Ir²,

Ca³

et al. 1991

Neuroendocrinology

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We have investigated the effect of adrenocorticotrophic hormone (ACTH) replacement after fetal hypophysectomy on the pattern of localization of enkephalin-containing peptides (enkephalins) and phenylethanolamine N-methyltransferase (PNMT) in the fetal sheep adrenal. We have also investigated the relative roles of the fetal pituitary and adrenal cortex in determining the extent of the interdigitation of the peripheral adrenaline (AD)-containing cells of the adrenal medulla with the inner zones of the adrenal cortex in the late gestation fetus. Fetal hypophysectomy (Hx; n = 12) or sham operations (n = 8) were performd at 109–118d. At 138 or 139d, ACTH (1–24) (10.5 µg/h) was infused intravenously for 72 h into 4 Hx fetuses (Hx + ACTH group). Saline was infused for 72 h into 4 Hx fetuses (Hx + Sal) and into 4 sham-operated fetal sheep (Intact + Sal). Fetal adrenal glands were collected at autopsy from 141/2d Intact + Sal, Hx + Sal and Hx + ACTH groups, from 4 intact fetal sheep at 145–147d gestation (145/7d Intact group) and 4 Hx fetal sheep at 147–164d gestation (147/64d Hx group). Adrenals were also collected from 4 newborn lambs at 10–12d afterbirth (10/12d Newborn group). Using the peroxidase-antiperoxidase immunocytochemical staining method, sections of adrenal glands (10–12 µm) from all groups were stained anti-PNMT. Sections of adrenal glands from the 141/2d groups were also stained separately with anti-dopamine-β-hydroxylase (anti-DβH) and anti-enkephalin (anti-ENK). Morphometric analyses were carried out on mid-glandular sections stained with anti-PNMT and an index of the interdigitation between the adrenal cortex and adrenal medulla was calculated (i.e. interdigitation index = the ratio of the perimeter of the adrenal medulla to the perimeter of a circle of the same area as the adrenal medulla). In the 141/2dHx + Sal and the 141/2dHx + ACTH groups, more central adrenomedullary cells were stained with anti-PNMT than in the intact group. In the 141/2d Intact + Sal group, staining with anti-ENK was observed in the peripheral rim of adrenal medulla cells which were adjacent to and interdigi-tated with the cells of the adrenal cortex. In the intact and Intact + SAL group, the intensity of staining with anti-ENK in the central noradrenaline (NA)-containing region was variable with many unstained cells present. In the 141/2d Hx + Sal and Hx + ACTH groups, however, staining for enkephalins was present throughout the entire adrenal medulla. The interdigitation index of the adrenal medulla and the adrenal cortex was significantly increased in the 10/ 12d Newborn (3.00 ± 0.07), 145/7d Intact (2.68 ± 0.07), and the 141/2d Hx + ACTH (2.62 ± 0.06) when compared to the 147/64d Hx (1.42 ± 0.03), 141/2d Intact + Sal (1.29 ± 0.02) and 141/2d Hx + Sal (1.15 ± 0.01) groups (p < 0.05). When the data were combined for all fetal and newborn lamb adrenals there was a positive correlation between the area of the adrenal cortex and the interdigitation index (r = 0.802; P < 0.001). In summary, we found that the pattern of localizatio...

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“…IGF-II is a potent mitogen in cultured fetal rat adrenocortical cells (Van Dijk et al, 1988), whereas IGF-I is an important regulator of postnatal adrenocortical growth. IGF-I may also play a role in the induction and maintenance of steroidogenic function of adrenocortical cells, since both IGF-I peptide and its receptors have been detected in the adrenal cortex of various species (Feige and Baird, 1991;Penhoat et al, 1988;Pillion et al, 1988Pillion et al, , 1989.…”

Section: Igf-i In the Adrenal Cortexmentioning

confidence: 99%

Peptide growth factors and the adrenal cortex

Vinson

1997

Microsc. Res. Tech.

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Insulin-like growth factor (IGF)-II and insulin, but not IGF-I, are mitogenic for fetal rat adrenal cells in vitro

Cited by 28 publications

References 58 publications

Role of adenylate cyclase-cyclic AMP-dependent signal transduction in the ACTH-induced biphasic growth effect of rat adrenocortical cells in primary culture

Role of adenylate cyclase-cyclic AMP-dependent signal transduction in the ACTH-induced biphasic growth effect of rat adrenocortical cells in primary culture

Different Roles for the Pituitary and Adrenal Cortex in the Control of Enkephalin Peptide Localization and Cortico-Medullary Interaction in the Sheep Adrenal during Development

Peptide growth factors and the adrenal cortex

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