Insulin Suppresses HDL-Mediated Cholesterol Efflux from Macrophages Through Inhibition of Neutral Cholesteryl Ester Hydrolase and ATP-Binding Cassette Transporter G1 Expressions

Abstract

Aims:We studied the effect of insulin on HDL-mediated cholesterol efflux from macrophages. The potential involvement of cholesteryl ester hydrolysis and membrane cholesterol transport was also addressed. Methods

Relationships between cholesterol efflux and high-density lipoprotein particles in patients with type 2 diabetes mellitus

“…Antihyperglycemic agents such as insulin, 10 metformin, thiazolidinediones, 26 and lipid-lowering therapies using statin, 27 fibrates, 28 and niacin 29 all have the potential to alter lipoprotein number, size, and distribution. HDL-mediated cholesterol efflux is also affected by insulin, 30 metformin, 31 and activators of the peroxisome proliferator-activated receptors. 32 In our study, most of our patients were on statins.…”

Reduction of paraoxonase-1 activity may contribute the qualitative impairment of HDL particles in patients with type 2 diabetes

“…We have been measuring cholesterol efflux with THP-1 derived macrophages labeled with [ 3 H]-cholesterol and human HDL as the acceptor [32,33]. In the current experiment, cholesterol efflux was investigated by considering both the quality (Efflux-hdl) and quantity (Efflux-whole) of HDL particles in the HDL fraction.…”

Metabolic syndrome, diabetes, and hyperuricemia

“…In a cross-sectional study [37], high levels of insulin sensitivity were inversely associated with the levels of TG-HDL-c ratio; and even in children and adolescents, the TG-HDL-c ratio is strongly associated with insulin resistance [38 & ]. Yamashita et al [39] provided the mechanism that insulin can inhibit HDL-mediated cholesterol efflux from human acute monocyte leukemia cell line derived macrophages through the inhibition of neutral cholesterol ester hydrolase and ATP-binding cassette transporter G1 expression in a vitro study.…”