Insulin Use in Pregnancy

Crombach, G.; Siebolds, Marcus; Mies, R.

doi:10.2165/00003088-199324020-00001

Cited by 13 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results show that the augmented insulin responsiveness present in early gestation completely switches to a normal condition by day 14 of pregnancy, a situation previously observed in rats on day 15 of gestation (43). This is comparable to what occurs in late second trimester of pregnancy when insulin requirements in type 1 diabetic women are similar to those before pregnancy (10). Therefore, at this stage of gestation, normal sensitivity to insulin in the presence of hyperphagia and hyperinsulinemia sets the appropriate scene for the active anabolic condition present in the mother.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Fat accumulation in the rat during early pregnancy is modulated by enhanced insulin responsiveness

Ramos

Crespo-Solans

Campo

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

Insulin sensitivity has been implicated in the variation of fat accumulation in early gestation by as-yet-unknown mechanisms. In the present study, we analyzed the insulin sensitivity of lipolysis and lipogenesis in lumbar adipocytes from rats at 0, 7, 14, and 20 days of gestation. In adipocytes of 7-day pregnant rats, we found a twofold decrease in both β-agonist (isoproterenol and BRL-37344)-stimulated lipolysis and β3-adrenoceptor protein but not in lipolysis initiated by forskolin or isobutylmethylxanthine, suggesting a modification of the lipolytic pathway at the receptor level. Whereas adipocytes from 7-day pregnant rats showed a twofold increase in fatty acid synthesis from glucose, those from 20-day pregnant animals displayed a decreased lipogenic activity. Insulin responsiveness of the lipolytic and lipogenic pathways was analyzed by dose-response experiments, giving evidence for the involvement of improved insulin responsiveness in the enhanced lipogenic and reduced lipolytic activities of adipocytes in early pregnancy. In contrast, insulin resistance is responsible for lower antilipolytic and lipogenic actions of insulin in late pregnant animals. In conclusion, the present study shows that enhanced adipose tissue insulin responsiveness during early pregnancy contributes to maternal fat accumulation, whereas decreased insulin responsiveness during late gestation modulates fat breakdown.

show abstract

Section: Discussionsupporting

confidence: 87%

“…Subsequently, blood samples were collected into heparinized tubes at 2. 5,5,10,15,20,30,45,60, and 90 min after glucose dose and placed on ice. Samples were centrifuged, and plasma was stored at Ϫ20°C until processed.…”

Section: Methodsmentioning

confidence: 99%

Fat accumulation in the rat during early pregnancy is modulated by enhanced insulin responsiveness

Ramos

Crespo-Solans

Campo

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…Women in White groups AO and A2 were treated by dietary means alone. White BO patients received additional insulin therapy according to guidelines described earlier [7].…”

Section: Curriculum Vitaementioning

confidence: 99%

Relationship between amniotic fluid insulin and maternal blood glucose concentrations in patients with carbohydrate intolerance during pregnancy

Crombach¹,

Hammerschmidt²,

Schmitz-Röckerath³

et al. 1996

Jpme

Self Cite

View full text Add to dashboard Cite

In contrast to maternal blood glucose, amniotic fluid insulin (AFI) directly reflects the functional state of the fetal pancreas. In a prospective study we evaluated the correlation of AFI with maternal metabolic control in 70 amniotic fluid specimens from 61 women having carbohydrate intolerance during pregnancy (White A n = 44, B0 n = 17). AFI was measured with the Insulin RIA 100 kit from Pharmacia (Freiburg). The normal range of AFI was established in 304 healthy pregnant women (16th-42nd gestational week). AFI concentrations increased by a factor of 1.5 to 2 during gestation reflecting the maturation of the fetal pancreas. Elevated AFI levels (> 97th centile) were found in 11% of normoglycemic diabetics and in 50% of women with insufficient metabolic control. Despite a high overall concordance (81%) no direct relationship could be found between fetal and maternal parameters. Patients with increased AFI values had a 5-fold higher rate of large-for-gestational age (LGA) infants than women with normal levels. This finding confirms the pathogenetic role of hyperinsulinism in the development of fetal macrosomia.

show abstract

“…During early pregnancy the maternal pancreas increases its insulin content (28) and the sensitivity to insulinotropic stimuli is enhanced (5), whereas insulin sensitivity is either unchanged or even enhanced, in both women (8,9) and rats (18). In contrast, an intense insulin resistant condition arises in late pregnancy (6,28), even though the sensitivity to insulinotropic stimuli remains enhanced (11,28).…”

mentioning

confidence: 99%

Differential metabolic response to 48 h food deprivation at different periods of pregnancy in the rat

López-Soldado

Betancor-Fernández

Herrera

2002

J. Physiol. Biochem.

View full text Add to dashboard Cite

Since during pregnancy the mother switches from an anabolic to a catabolic condition, the present study was addressed to determine the effect of 48 h food deprivation on days 7, 14 and 20 of pregnancy in the rat as compared to age matched virgin controls. Body weight, free of conceptus, decreased with food deprivation more in pregnant than in virgin rats, with fetal weight (day 20) also diminishing with maternal starvation. The decline of plasma glucose with food deprivation was greatest in 20 day pregnant rats. Insulin was highest in fed 14 day pregnant rats, and declined with food deprivation in all the groups, the effect being not significant in 7-day pregnant rats. Food deprivation increased plasma glycerol only in virgin and 20 day pregnant rats. Plasma NEFA and 3-hydroxybutyrate increased with food deprivation in all groups, the effect being highest in 20 day pregnant rats. Food deprivation decreased plasma triacylglycerols in 14 day pregnant rats but increased in 20 day pregnant rats. In 20-day fetuses, plasma levels of glucose, NEFA and triacylglycerols were lower than in their mothers when fed, and food deprivation caused a further decline in plasma glucose, whereas both NEFA and 3-hydroxybutyrate increased. Liver triacylglycerols concentration did not differ among the groups when fed, whereas food deprivation caused an increase in all pregnant rats and fetuses, the effect being highest in 20-day pregnant rats. Lipoprotein lipase (LPL) activity in adipose tissue was lower in 20 day pregnant rats than in any of the other groups when fed, and it decreased in all the groups with food deprivation, whereas in liver it was very low in all groups when fed and increased with food deprivation only in 20 day pregnant rats. A significant increase in liver LPL was found with food deprivation in 20 day fetuses, reaching higher values than their mothers. Thus, the response to food deprivation varies with the time of pregnancy, being lowest at mid pregnancy and greatest at late pregnancy, and although fetuses respond in the same direction as their mothers, they show a specific response in liver LPL activity.

show abstract

Insulin Use in Pregnancy

Cited by 13 publications

References 34 publications

Fat accumulation in the rat during early pregnancy is modulated by enhanced insulin responsiveness

Fat accumulation in the rat during early pregnancy is modulated by enhanced insulin responsiveness

Relationship between amniotic fluid insulin and maternal blood glucose concentrations in patients with carbohydrate intolerance during pregnancy

Differential metabolic response to 48 h food deprivation at different periods of pregnancy in the rat

Contact Info

Product

Resources

About