2015
DOI: 10.1021/acs.bioconjchem.5b00036
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Intact Reducing Glycan Promotes the Specific Immune Response to Lacto-N-neotetraose-BSA Neoglycoconjugates

Abstract: The mammalian immune system responds to eukaryotic glycan antigens during infections, cancer, and autoimmune disorders, but the immunological bases for such responses are unclear. Conjugate vaccines containing bacterial polysaccharides linked to carrier proteins (neoglycoconjugates) have proven successful, but these often contain repeating epitopes and the reducing end of the glycan is less important, unlike typical glycan determinants in eukaryotes, which are shorter in length and may include the reducing end… Show more

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Cited by 16 publications
(18 citation statements)
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“…A major drawback of using DAP, AEAB, 2-AA, and 2-AB is that their conjugation reaction relies on reductive amination which, although highly efficient, opens the sugar ring at the reducing end monosaccharide (Figure 1). This destroys the reducing end integrity of the glycan (Prasanphanich et al, 2015) and potentially results in non-natural presentation of glycans on the array. This would affect the binding affinities of smaller glycans, and particularly those with modifications in the core region, such as core Fuc on N-glycans (Prasanphanich et al, 2015).…”
Section: Development Of Fluorescent Bi-functional Linkermentioning
confidence: 99%
See 1 more Smart Citation
“…A major drawback of using DAP, AEAB, 2-AA, and 2-AB is that their conjugation reaction relies on reductive amination which, although highly efficient, opens the sugar ring at the reducing end monosaccharide (Figure 1). This destroys the reducing end integrity of the glycan (Prasanphanich et al, 2015) and potentially results in non-natural presentation of glycans on the array. This would affect the binding affinities of smaller glycans, and particularly those with modifications in the core region, such as core Fuc on N-glycans (Prasanphanich et al, 2015).…”
Section: Development Of Fluorescent Bi-functional Linkermentioning
confidence: 99%
“…This destroys the reducing end integrity of the glycan (Prasanphanich et al, 2015) and potentially results in non-natural presentation of glycans on the array. This would affect the binding affinities of smaller glycans, and particularly those with modifications in the core region, such as core Fuc on N-glycans (Prasanphanich et al, 2015). We, therefore, have been developing new strategies to overcome this weakness while retain the merits mentioned above.…”
Section: Development Of Fluorescent Bi-functional Linkermentioning
confidence: 99%
“…In addition, a short peptide (UPK3A 65-84) from Uroplakin 3A (UPK3A) covalently coupled with KLH and CpG as adjuvant was found to be an immunotherapeutic vaccine for bladder cancer (Izgi et al, 2015). In addition, BSA is often used as a carrier protein for small antigens in glycoconjugate vaccines (Prasanphanich et al, 2015). For example, Cai et al combined a synthesized MUC1 glycopeptide with BSA or three different T-helper cell epitopes of TTox and demonstrated a beneficial effect (Cai et al, 2013).…”
Section: Keyhole Limpet Hemocyanin (Klh) and Bovine Serum Albumin (Bsa)mentioning
confidence: 99%
“…Even if the reducing end is preserved, the next step to immobilize these glycans often requires ring-opening tagging, which significantly affects the structural integrities of the O-glycans due to their relatively smaller size compared to N-glycans. The reducing end of glycans is often critical to their recognition by antibodies and other molecules [52]. For O-glycans the reducing end GalNAc often serves as a branching point for O-glycans.…”
Section: Preparation Of O-glycans For Glycan Microarraymentioning
confidence: 99%