Integrated analysis to reveal potential therapeutic targets and prognostic biomarkers of skin cutaneous melanoma

Abstract

Skin cutaneous melanoma (SKCM) is a malignant tumor with high mortality rate in human, and its occurrence and development are jointly regulated by genes and the environment. However, the specific pathogenesis of SKCM is not completely understood. In recent years, an increasing number of studies have reported the important role of competing endogenous RNA (ceRNA) regulatory networks in various tumors; however, the complexity and specific biological effects of the ceRNA regulatory network of SKCM remain unclear. In the present study, we obtained a ceRNA regulatory network of long non-coding RNAs, microRNAs, and mRNAs related to the phosphatase and tensin homolog (PTEN) in SKCM and identified the potential diagnostic and prognostic markers related to SKCM. We extracted the above three types of RNA involved in SKCM from The Cancer Genome Atlas database. Through bioinformatics analysis, the OIP5-AS1-hsa-miR-186-5p/hsa-miR-616-3p/hsa-miR-135a-5p/hsa-miR-23b-3p/hsa-miR-374b-5p-PTPRC/IL7R/CD69 and MALAT1-hsa-miR-135a-5p/hsa-miR-23b-3p/hsa-miR-374b-5p-IL7R/CD69 ceRNA networks were found to be related to the prognosis of SKCM. Finally, we determined the OIP5-AS1-PTPRC/IL7R/CD69 and MALAT1-IL7R/CD69 axes in ceRNA as a clinical prognostic model using correlation and Cox regression analyses. Additionally, we explored the possible role of these two axes in affecting gene expression and immune microenvironment changes and the occurrence and development of SKCM through methylation and immune infiltration analyses. In summary, the ceRNA-based OIP5-AS1-PTPRC/IL7R/CD69 and MALAT1-IL7R/CD69 axes may be a novel and important approach for the diagnosis and prognosis of SKCM.

Identification and validation of non‐coding RNA‐mediated high expression of IQGAP3 in poor prognosis of lung adenocarcinoma

“…also reported that there was a significant difference in hsa‐miR‐135a‐5p expression in the peripheral blood between lung cancer patients and healthy controls. In addition, hsa‐miR‐135a‐5p‐mediated ceRNA networks were also found to be related to the prognosis of skin cutaneous melanoma 22 …”

“…In addition, hsa-miR-135a-5p-mediated ceRNA networks were also found to be related to the prognosis of skin cutaneous melanoma. 22 Research on ceRNAs in lung cancer will provide new insights into cancer pathogenesis and treatment options. 23 It has been suggested that lncRNAs can act as sponges with miRNAs to control the expression of target genes.…”

Predicting immunotherapy response in melanoma using a novel tumor immunological phenotype-related gene index

“…In 2021, Thomas F Gajewski and colleagues reported that up to 90% of PTPRC+ cells produced CXCL10 transcripts, which played a critical role in recruiting effector CD8+ T cells to the tumor site ( 27 ). In 2022, Xiaobo Xia and colleagues reported that the OIP5-AS1-PTPRC/IL7R/CD69 axis in ceRNA as a clinical prognostic model ( 28 ).…”

Primary and Metastatic Cutaneous Melanomas Discriminately Enrich Several Ligand-Receptor Interactions

“…Cutaneous melanoma (CM) remains one of the most aggressive and deadliest forms of skin cancer, with epidemiological studies depicting the last few decades witnessing dramatic increases in incidences worldwide, with over 200,000 new cases diagnosed each year [ 1 , 2 ]. However, the morbidity and mortality rates of CM globally vary significantly, contingent on the availability of early screening and primary care [ 3 ]. This skin malignant tumor exhibits high metastasis, often leading to poor prognosis, as patients diagnosed with late-stage CM face a 5-year survival rate of less than 10% [ 4 ].…”