Integrated Approach To Producing High-Purity Trehalose from Maltose by the Yeast Yarrowia lipolytica Displaying Trehalose Synthase (TreS) on the Cell Surface

Abstract

An alternative strategy that integrated enzyme production, trehalose biotransformation, and bioremoval in one bioreactor was developed in this study, thus simplifying the traditional procedures used for trehalose production. The trehalose synthase gene from a thermophilic archaea, Picrophilus torridus, was first fused to the YlPir1 anchor gene and then inserted into the genome of Yarrowia lipolytica, thus yielding an engineered yeast strain. The trehalose yield reached 73% under optimal conditions. The thermal and pH stabilities of the displayed enzyme were improved compared to those of its free form purified from recombinant Escherichia coli. After biotransformation, the glucose byproduct and residual maltose were directly fermented to ethanol by a Saccharomyces cerevisiae strain. Ethanol can be separated by distillation, and high-purity trehalose can easily be obtained from the fermentation broth. The results show that this one-pot procedure is an efficient approach to the economical production of trehalose from maltose.

A Novel Trehalose Synthase for the Production of Trehalose and Trehalulose

“…However, increasing the substrate (sucrose) concentration (300 mM to 1 M) negatively affected the catalytic conversion (Fig. S4B), which could be due to the increased viscosity in the reaction mixture, affecting the protein folding and kinetic performance of the enzyme ( 57 ). The utilization of low-cost feedstock (table sugar, muscovado, and sweet sorghum stalk juice) as glycosyl donors in the enzymatic reaction further validated the industrial importance of the novel trehalose synthase TreM, achieving a trehalulose yield comparable to that of sucrose.…”

Highly Efficient Erythritol Recovery from Waste Erythritol Mother Liquor by a Yeast-Mediated Biorefinery Process

“…There is a substantial demand for new yeast cell factories which can synthesize target compounds more efficiently and in higher yields than chemical synthesis. Engineered microbial cell factories, especially those of yeast cells, are economically feasible and sustainable for the production of various fine chemicals and functional sugars. − …”

Whole Conversion of Soybean Molasses into Isomaltulose and Ethanol by Combining Enzymatic Hydrolysis and Successive Selective Fermentations

“…To further reduce the carbohydrate byproducts and improve the isomaltulose purity, four ethanol-producing yeast strains, including P. stipitis, S. cerevisiae, H. uvarum , and K. marxianu , were used to check their ability to eliminate the residual galactose and xylose as Li description with some changes [26]. Y. lipolytica was used as a control.…”