Integrated cascade biorefinery processes for the production of single cell oil by Lipomyces starkeyi from Arundo donax L. hydrolysates

Fidio, Nicola Di; Ragaglini, Giorgio; Dragoni, Federico; Antonetti, Claudia; Galletti, Anna Maria Raspolli

doi:10.1016/j.biortech.2020.124635

Cited by 33 publications

(25 citation statements)

References 44 publications

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“…The remaining fatty acids included stearic acid (C18:0) with a content of 4.59–5.97%, palmitoleic acid (C16:1) with a content of 3.01–3.96%, and linoleic acid (C18:2) with a content of 1.12–2.93%, respectively. Such a fatty acids profile, similar to the main components of vegetable oil, was consistent with several previous studies [ 42 , 43 , 44 ]. The differences in fatty acids profiles among different fermentation flasks were analysed by significance analysis.…”

Section: Resultssupporting

confidence: 92%

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

et al. 2021

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The high operating cost is currently a limitation to industrialize microbial lipids production by the yeast Lipomyces starkeyi. To explore economic fermentation technology, the two-stage fermentation of Lipomyces starkeyi using yeast extract peptone dextrose (YPD) medium, orange peel (OP) hydrolysate medium, and their mixed medium were investigated for seven days by monitoring OD600 values, pH values, cell growth status, C/N ratios, total carbon concentration, total nitrogen concentration, residual sugar concentration, lipid content, lipid titer, and fatty acids profiles of lipids. The results showed that two-stage fermentation with YPD and 50% YPD + 50% OP medium contributed to lipid accumulation, leading to larger internal lipid droplets in the yeast cells. However, the cells in pure OP hydrolysate grew abnormally, showing skinny and angular shapes. Compared to the one-stage fermentation, the two-stage fermentation enhanced lipid contents by 18.5%, 27.1%, and 21.4% in the flasks with YPD medium, OP medium, and 50%YPD + 50%OP medium, and enhanced the lipid titer by 77.8%, 13.6%, and 63.0%, respectively. The microbial lipids obtained from both one-stage and two-stage fermentation showed no significant difference in fatty acid compositions, which were mainly dominated by palmitic acid (33.36–38.43%) and oleic acid (46.6–48.12%). Hence, a mixture of commercial medium and lignocellulosic biomass hydrolysate could be a promising option to balance the operating cost and lipid production.

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Section: Resultssupporting

confidence: 92%

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

et al. 2021

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“…The complex structure of lignocellulosic biomass and its innate recalcitrance to chemical and/or biological depolymerisation require multistep processes in order to convert the starting biomass into added-value products. The most common biorefinery schemes are based on the initial pretreatment of lignocellulosic biomass in order to deconstruct the lignocellulose matrix and make it more prone to subsequent chemical or biological hydrolysis [4,7,15,92,93]. Table S1 in Supplementary Materials shows a short list of the most common pretreatment approaches of lignocellulosic biomass together with a brief description of their main advantages and disadvantages.…”

Section: Lignocellulosic Biomassesmentioning

confidence: 99%

“…These renewable resources are often represented by agro-industrial side-streams, which have to be converted to added-value compounds in the perspective of integrated industrial models and a circular economy [3]. Moreover, in order to ensure the economic and environmental sustainability of biorefineries, the complete valorisation of the raw starting materials by green-process technology should be implemented [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…A crucial aspect to guarantee the economic sustainability of yeasts oil production is represented by the use of low-or negative-value carbon sources deriving from secondgeneration biomasses and/or agro-industrial side-streams [22]. For example, the use of sugar-containing hydrolysates from lignocellulosic crops is one the most common approaches [3,4,7,23,24]. However, the quality of biomass hydrolysates is essential for good production of TAGs, as the presence of furanic compounds deriving from the degradation of sugars, such as furfural and 5-hydroxymethylfurfural, can significantly alter yeast growth and oil yield [25,26].…”

Section: Introductionmentioning

confidence: 99%

“…In fact, some organic compounds, such as furaldehyde, formaldehyde, phenols, aliphatic acids, vanillic acid, uronic acid, 4-hydroxybenzoic acid, acetic acid and cinnamaldehyde, can act as inhibitors for different metabolic activities of oleaginous yeasts [27,28]. The susceptibility of microorganisms to inhibitors strictly depends on the strain they belong to, so it is necessary to adapt the fermentation process to the type of microorganism used, in order to increase the cell biomass and lipid production [4,7,17].…”

Section: Introductionmentioning

confidence: 99%

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Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

et al. 2021

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Cutaneotrichosporon oleaginosus is an oleaginous yeast with several favourable qualities: It is fast growing, accumulates high amounts of lipids and has a very broad substrate spectrum. Its resistance to hydrolysis by-products makes it a promising biocatalyst for custom tailored microbial oils. C. oleaginosus can accumulate up to 60 wt.% of its biomass as lipids. This species is able to grow by using several compounds as a substrate, such as acetic acid, biodiesel-derived glycerol, N-acetylglucosamine, lignocellulosic hydrolysates, wastepaper and other agro-industrial wastes. This review is focused on state-of-the-art innovative and sustainable biorefinery schemes involving this promising yeast and second- and third-generation biomasses. Moreover, this review offers a comprehensive and updated summary of process strategies, biomass pretreatments and fermentation conditions for enhancing lipid production by C. oleaginosus as a whole-cell biocatalyst. Finally, an overview of the main industrial applications of single-cell oil is reported together with future perspectives.

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Sustainable Valorisation and Efficient Downstream Processing of Giant Reed by High‐Pressure Carbon Dioxide Pretreatment

et al. 2022

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This work investigated the catalytic high‐pressure CO2 pretreatment of giant reed. CO2 is a renewable resource; its use does not generate chemical wastes and it can be easily removed and recycled. The effect of the addition of low concentrations of FeCl3 (0.16 wt %) and PEG 400 (1.0 wt %) on the hemicellulose hydrolysis to xylose and xylo‐oligosaccharides (XOS) is reported for the first time. Under the optimised pretreatment conditions, the xylan conversion of 82 mol % and xylose and XOS yields of 43 and 20 mol % were achieved, respectively. The solid residues obtained from different pretreatments were used as the substrate for the enzymatic hydrolysis to give glucose. The total glucose yield achieved under the optimised two‐step process was 67.8 mol % with respect to the glucan units in the biomass. The results demonstrated that PEG‐assisted FeCl3‐catalysed scCO2 pretreatment can produce xylose‐ or XOS‐rich hydrolysates and improve the enzymatic hydrolysis of biomass.

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Integrated cascade biorefinery processes for the production of single cell oil by Lipomyces starkeyi from Arundo donax L. hydrolysates

Cited by 33 publications

References 44 publications

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

Two-Stage Fermentation of Lipomyces starkeyi for Production of Microbial Lipids and Biodiesel

Cutaneotrichosporon oleaginosus: A Versatile Whole-Cell Biocatalyst for the Production of Single-Cell Oil from Agro-Industrial Wastes

Sustainable Valorisation and Efficient Downstream Processing of Giant Reed by High‐Pressure Carbon Dioxide Pretreatment

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