Integrated dual-color stimulated emission depletion (STED) microscopy and fluorescence emission difference (FED) microscopy

Wang, Wensheng; Zhao, Guangyuan; Kuang, Cuifang; Xu, Liang; Liu, Shaocong; Sun, Shi-Hai; Shentu, Ping; Yang, Yang; Xu, Yingke; Liu, Xü

doi:10.1016/j.optcom.2018.04.017

Cited by 9 publications

(2 citation statements)

References 29 publications

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“…Point-like objects with diameters smaller than the system resolution are usually used to directly measure system resolution. The reported point-like objects include fluorescent beads (200 nm, 100 nm or smaller) , special point matrix samples (Wang et al, 2018), nanorulers (Raab et al, 2018), and many others (Farahi, 2015). System resolution is given by the FWHM of a fluorescence image from a point-like object or the minimal distance between two adjacent pointlike objects.…”

Section: Direct Measurement Of System Resolutionmentioning

confidence: 99%

Rethinking resolution estimation in fluorescence microscopy: from theoretical resolution criteria to super-resolution microscopy

Huang

2020

Sci. China Life Sci.

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Resolution is undoubtedly the most important parameter in optical microscopy by providing an estimation on the maximum resolving power of a certain optical microscope. For centuries, the resolution of an optical microscope is generally considered to be limited only by the numerical aperture of the optical system and the wavelength of light. However, since the invention and popularity of various advanced fluorescence microscopy techniques, especially super-resolution fluorescence microscopy, many new methods have been proposed for estimating the resolution, leading to confusions for researchers who need to quantify the resolution of their fluorescence microscopes. In this paper, we firstly summarize the early concepts and criteria for predicting the resolution limit of an ideal optical system. Then, we discuss some important influence factors that deteriorate the resolution of a certain fluorescence microscope. Finally, we provide methods and examples on how to measure the resolution of a fluorescence microscope from captured fluorescence images. This paper aims to answer as best as possible the theoretical and practical issues regarding the resolution estimation in fluorescence microscopy.

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Section: Direct Measurement Of System Resolutionmentioning

confidence: 99%

Rethinking resolution estimation in fluorescence microscopy: from theoretical resolution criteria to super-resolution microscopy

Huang

2020

Sci. China Life Sci.

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“…The combination of Förster resonance energy transfer (FRET) with multiparameter fluorescence detection (MFD), fluorescence correlation spectroscopy (FCS), and computational techniques allows the probing of protein conformational dynamics at high resolutions in both space and time (12)(13)(14)(15)(16). Integration of stimulated emission depletion (STED) and fluorescence emission difference (FED) capabilities allows researchers to probe biological systems below diffraction-limited resolutions (17)(18)(19). Further, the combination of STED and FCS allows probing the dynamics in living cells below the diffraction limit with high time resolution (20,21).…”

Section: Introductionmentioning

confidence: 99%

Reporting on the future of integrative structural biology ORAU workshop

Sanabria¹

2020

Front Biosci

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Integrative and hybrid methods have the potential to bridge long-standing knowledge gaps in structural biology. These methods will have a prominent role in the future of the field as we make advances toward a complete, unified representation of biology that spans the molecular and cellular scales. The Department of Physics and Astronomy at Clemson University hosted The Future of Integrative Structural Biology workshop on April 29, 2017 and partially sponsored by partially sponsored by a program of the Oak Ridge Associated Universities (ORAU). The workshop brought experts from multiple structural biology disciplines together to discuss nearterm steps toward the goal of a molecular atlas of the cell. The discussion focused on the types of structural data that should be represented, how this data should be represented, and how the time domain might be incorporated into such an atlas. The consensus was that an explorable, map-like Virtual Cell, containing both spatial and temporal data bridging the atomic and cellular length scales obtained by multiple experimental methods, represents the best path toward a complete atlas of the cell.

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