Integrated Graphene Oxide with Noble Metal Nanoparticles to Develop High-Sensitivity Fiber Optic Particle Plasmon Resonance (FOPPR) Biosensor for Biomolecules Determination

Abstract

In this research, a direct, simple and ultrasensitive fiber optic particle plasmon resonance (FOPPR) biosensing platform for immunoglobulin G (IgG) detection was developed using a gold nanoparticle/graphene oxide (AuNP/GO) composite as signal amplification element. To obtain the best analytical performance of the sensor, experimental parameters including the surface concentration of GO on the AuNPs, formation time of the GO, the concentration of the anti-IgG and incubation time of anti-IgG were optimized. The calibration plots displayed a good linear relationship between the sensor response (ΔI/I0) and the logarithm of the analyte concentrations over a linear range from 1.0 × 10−10 to 1.0 × 10−6 g/mL of IgG under the optimum conditions. A limit of detection (LOD) of 0.038 ng/mL for IgG was calculated from the standard calibration curve. The plot has a linear relationship (correlation coefficient, R = 0.9990). The analytical performance of present work’s biosensor was better than that of our previously reported mixed self-assembled monolayer of 11-mercaptoundecanoic acid/6-mercapto-1-hexanol (MUA/MCH = 1:4) method by about three orders of magnitude. The achieved good sensitivity may be attributed to the synergistic effect between GO and AuNPs in this study. In addition, GO could immobilize more antibodies due to the abundant carboxylic groups on its surface. Furthermore, we also demonstrated that the results from this sensor have good reproducibility, with coefficients of variation (CVs) < 8% for IgG. Therefore, the present strategy provides a novel and convenient method for chemical and biochemical quantification and determination.

Determination of the Highly Sensitive Carboxyl-Graphene Oxide-Based Planar Optical Waveguide Localized Surface Plasmon Resonance Biosensor

“…It was obvious that the GO-OW LSPR biosensor had excellent detection sensitivity. The order of magnitude is the same as the sensitivity of the AuNP-GO-Anti-IgG FOPPR sensor reported earlier [46].…”

“…Figure 3c shows the FESEM image of the GO. The result shows a typical thin, corrugated, and papery GO structure [46]. Figure 3d shows an FESEM image of the modified AuNPs-GO on the sensing chip surface observed through FESEM.…”

“…The result showed that the LOD for the buffer solution sample was 1 pM and that for the serum sample was 1.9 pM [45]. In our previous study [46], GO was used to increase the sensitivity of fiber-optic localized surface plasmon resonance. The result showed that the immunoglobulin G (IgG) molecule detection sensitivity was 0.038 ng/mL.…”

Flexible and Ultrathin Metal-Oxide Films for Multiresonance-Based Sensors in Plastic Optical Fibers

“…Along this line, several configurations have been exploited in literature, like the ones based on Dshaped fibers [14][15][16][17], tapered fibers [18][19][20][21], U-bent fibers [22,23], and many others [24][25][26]. In the last years, in order to improve the performances of SPR sensors based on optical fiber, several measures have been adopted, like, for instance, by using ultra-thin films graphene-based, that have led to excellent results [27][28][29].…”

“…To realize even more efficient sensor systems, SPR, LSPR, and LMR can be coupled with optical fibers so taking advantage of the excellent properties related to these guiding structures, such as immunity to electromagnetic interferences, the possibility of remote sensing, and so on. − Along this line, several configurations have been exploited in the literature, such as the ones based on D-shaped fibers, − tapered fibers, − U-bent fibers, , and many others. − In the last years, to improve the performances of SPR sensors based on optical fiber, several measures have been adopted, such as, for instance, using ultrathin-film graphene-based, that have led to excellent results. − …”