Integrated “lab-on-a-chip” microfluidic systems for isolation, enrichment, and analysis of cancer biomarkers

Surappa, Sushruta; Multani, Priyanka; Parlatan, Ugur; Sinawang, Prima Dewi; Kaifi, Jussuf T.; Akin, Demir; Demirci, Utkan

doi:10.1039/d2lc01076c

Cited by 23 publications

(8 citation statements)

References 201 publications

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“…Microfluidic devices have been widely used to handle particles (either synthetic or biological) for chemical, biomedical, and environmental applications. − Focusing particles into a narrow stream is usually a necessary step in many of these devices such as a flow cytometer and a particle sorter. − Both externally imposed (e.g., acoustic, − electric, − magnetic, − etc.) and internally induced (e.g., inertial − and elastic − lift) forces have been utilized to move particles across streamlines for sheath-free focusing.…”

Section: Introductionmentioning

confidence: 99%

Fluid Elasticity-Enhanced Insulator-Based Dielectrophoresis for Sheath-Free Particle Focusing in Very Dilute Polymer Solutions

Raihan,

Baghdady,

Dort

et al. 2023

Anal. Chem.

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Focusing particles into a narrow stream is usually a necessary step in microfluidic flow cytometry and particle sorting. We demonstrate that the addition of a small amount of poly(ethylene oxide) (PEO) polymer into a buffer solution can reduce by almost 1 order of magnitude the threshold DC electric field for single-line dielectrophoretic focusing of particles in a constricted microchannel. The particle focusing effectiveness of this fluid elasticity-enhanced insulator-based dielectrophoresis (E-iDEP) in very dilute PEO solutions gets enhanced with the increase of the PEO molecular weight and particle size. These two trends are consistent with a theoretical analysis that accounts for the fluid elasticity effects on the electrokinetic and dielectrophoretic particle motions. Surprisingly, the particle-focusing effectiveness of E-iDEP is observed to first increase and then decrease with an increase in the PEO concentration.

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Section: Introductionmentioning

confidence: 99%

Fluid Elasticity-Enhanced Insulator-Based Dielectrophoresis for Sheath-Free Particle Focusing in Very Dilute Polymer Solutions

Raihan,

Baghdady,

Dort

et al. 2023

Anal. Chem.

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“…New microfluidic LOC platforms are always under development with the goals of making isolation more sensitive and making in situ analysis even easier. The present trend and desire for the development of "smart" systems that are both highly functional and easy to use, especially for those without a background in POCT, are congruent with this [23]. Furthermore, the initial stride towards individualized healthcare may lie in the development, standardization, and utilization of such cutting-edge technology.…”

Section: Discussionmentioning

confidence: 91%

“…The potential of exosomes as a fluid biopsy for the identification of cancer biomarkers has garnered increasing attention during the past decade. Research like these has shown how critical it is to develop innovative methods for accurately and quickly separating exosomes from bodily fluids with little sample preparation time required [21][22][23][24][25]. Serious problems with purity and more intact performance arise when exosomes are exposed to contaminants and mechanical damage.…”

Section: Discussionmentioning

confidence: 99%

A Microchip For Exosome Isolation That Can Be Impregnated With Imatinib Simultaneously: An In Vitro Analysis

Monfaredan,

Rahim,

Tavoosidana

et al. 2024

Russ Open Med J

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Background and Aims — Exosomes, which are tiny double-layered membranes originating from eukaryotic cells, have been recognized as a valuable natural vehicle for delivering substances because of their optimal size, compatibility with living organisms, strong structure, ability to carry a large amount of cargo, and capacity to be modified on their surface. Methods — Various strategies have been employed to isolate exosomes due to the challenges associated with maintaining their high purity. The current investigation utilized a soft lithography technique to fabricate channels for exosome separation, incorporating immunoaffinity capabilities. Both biochemical and biophysical assays were conducted to assess the quality of isolated exosomes from various sources (serum, cell supernatant, and urine) and compared with a commercially available kit. Results — The current investigation employed a microfluidic method to capture CD63-conjugated magnetic beads, resulting in a very effective separation of exosomes. Based on the data, there were no notable variations in miRNAs that were statistically significant. This demonstrates that the engineered chip successfully achieved the separation of the exosome while preserving the integrity of its nucleic acid components. Conclusion — The results shown that the current methodology effectively isolated exosomes with a high yield rate, purity, and minimal time requirement. The imatinib laden exosomes demonstrated anticancer efficacy against the KYO-1 cell line in all of their forms.

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“…Microfluidic technologies, sometimes referred to as "lab-on-a-chip" provide an opportunity to create devices or chips that could compete with classical techniques in biomedical and chemical research. [149][150][151][152] These low-cost devices can process microliters of solution via capillary channels in a high-throughput manner, thus reducing the requirement for samples and reagents. Importantly, all reactions and washing steps are performed in sealed reaction systems, ideal for preventing cross-contamination.…”

Section: Microfluidics and Lab-on-a-chip Technology-integrating Indiv...mentioning

confidence: 99%

Advanced technologies towards improved HPV diagnostics

Bartosik,

Moranova,

Izadi

et al. 2024

Journal of Medical Virology

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Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause of cervical cancer, and an important factor in other malignancies, for example, head and neck cancer. Despite recent progress in screening and vaccination, the incidence and mortality are still relatively high, especially in low‐income countries. The mortality and financial burden associated with the treatment could be decreased if a simple, rapid, and inexpensive technology for HPV testing becomes available, targeting individuals for further monitoring with increased risk of developing cancer. Commercial HPV tests available in the market are often relatively expensive, time‐consuming, and require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies are being implemented also for HPV diagnostics that include for example, isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well as microfluidics, paperfluidics and lab‐on‐a‐chip devices, ideal for point‐of‐care testing in decentralized settings. In this review, we first evaluate current commercial HPV tests, followed by a description of advanced technologies, explanation of their principles, critical evaluation of their strengths and weaknesses, and suggestions for their possible implementation into medical diagnostics.

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Integrated “lab-on-a-chip” microfluidic systems for isolation, enrichment, and analysis of cancer biomarkers

Abstract: Liquid biopsy has garnered considerable attention as a complementary clinical tool for the early detection, molecular characterization and monitoring of cancer over the past decade. In contrast to traditional solid...

Cited by 23 publications

References 201 publications

Fluid Elasticity-Enhanced Insulator-Based Dielectrophoresis for Sheath-Free Particle Focusing in Very Dilute Polymer Solutions

Fluid Elasticity-Enhanced Insulator-Based Dielectrophoresis for Sheath-Free Particle Focusing in Very Dilute Polymer Solutions

A Microchip For Exosome Isolation That Can Be Impregnated With Imatinib Simultaneously: An In Vitro Analysis

Advanced technologies towards improved HPV diagnostics

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