Integrated lateral flow immunoassays using trimethylsilyl cellulose barriers for the enhanced sensitivity of COVID-19 diagnosis

Kumsab, Jakkaphan; Deenin, Wanwisa; Yakoh, Abdulhadee; Pimpitak, Umaporn; Amornkitbamrung, Lunjakorn; Rengpipat, Sirirat; Hirankarn, Nattiya; Crespo, Gastón A.; Chaiyo, Sudkate

doi:10.1016/j.jsamd.2023.100620

Cited by 3 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean concentration of antiHCII was 46. 44 Regarding urine samples, there was no statistically significant difference in protein levels between groups (Figure 2B). The concentrations of antiHCII, antiORP150, and antiAPOA1 in urine were 31.00 ng/mL (4.01, 0.00-216.44), 17.17 ng/mL (0.39, 0.00-153.64), and 17.43 ng/mL (12.73, 0.67-64.72) in moderate COVID-19 patients, respectively, and those were 26.45 ng/mL (5.48, 0.00-186.83), 15.29 ng/mL (3.12, 0.00-136.43), and 21.65 ng/mL (15.93, 0.63-113.36) in severe COVID-19 patients, respectively.…”

Section: Elisa Resultsmentioning

confidence: 86%

“…Various serological tests are available for diagnosing COVID-19, each employing different techniques such as direct or indirect enzyme-linked immunosorbent assay (ELISA) ( 36 – 38 ), immunochromatographic assay ( 39 , 40 ), chemiluminescent immunoassay (CLIA) ( 38 , 41 – 43 ), lateral flow analysis ( 44 – 46 ), and others. All these techniques aim to detect specific antibodies to the SARS-CoV-2 virus.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of serum and urine biomarkers for severe COVID-19

Shansky,

Yanushevich,

Gospodarik

et al. 2024

Front. Med.

View full text Add to dashboard Cite

IntroductionThe new coronavirus disease, COVID-19, poses complex challenges exacerbated by several factors, with respiratory tissue lesions being notably significant among them. Consequently, there is a pressing need to identify informative biological markers that can indicate the severity of the disease. Several studies have highlighted the involvement of proteins such as APOA1, XPNPEP2, ORP150, CUBN, HCII, and CREB3L3 in these respiratory tissue lesions. However, there is a lack of information regarding antibodies to these proteins in the human body, which could potentially serve as valuable diagnostic markers for COVID-19. Simultaneously, it is relevant to select biological fluids that can be obtained without invasive procedures. Urine is one such fluid, but its effect on clinical laboratory analysis is not yet fully understood due to lack of study on its composition.MethodsMethods used in this study are as follows: total serum protein analysis; ELISA on moderate and severe COVID-19 patients’ serum and urine; bioinformatic methods: ROC analysis, PCA, SVM.Results and discussionThe levels of antiAPOA1, antiXPNPEP2, antiORP150, antiCUBN, antiHCII, and antiCREB3L3 exhibit gradual fluctuations ranging from moderate to severe in both the serum and urine of COVID-19 patients. However, the diagnostic value of individual anti-protein antibodies is low, in both blood serum and urine. On the contrary, joint detection of these antibodies in patients’ serum significantly increases the diagnostic value as demonstrated by the results of principal component analysis (PCA) and support vector machine (SVM). The non-linear regression model achieved an accuracy of 0.833. Furthermore, PCA aided in identifying serum protein markers that have the greatest impact on patient group discrimination. The study revealed that serum serves as a superior analyte for describing protein quantification due to its consistent composition and lack of organic salts and drug residues, which can otherwise affect protein stability.

show abstract

Section: Elisa Resultsmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Evaluation of serum and urine biomarkers for severe COVID-19

Shansky,

Yanushevich,

Gospodarik

et al. 2024

Front. Med.

View full text Add to dashboard Cite

show abstract

Systematic optimization of UCNPs-LFA for Helicobacter pylori nucleic acid detection at point-of-care

Jin,

Li,

Zhang

et al. 2024

Microchim Acta

View full text Add to dashboard Cite

Lateral Flow Immunosensing of Salmonella Typhimurium Cells in Milk: Comparing Three Sequences of Interactions

Byzova,

Safenkova,

Gorbatov

et al. 2024

Microorganisms

View full text Add to dashboard Cite

To ensure the safety of foodstuffs, widespread non-laboratory monitoring for pathogenic contaminants is in demand. A suitable technique for this purpose is lateral flow immunoassay (LFIA) which combines simplicity, rapidity, and productivity with specific immune detection. This study considered three developed formats of LFIA for Salmonella Typhimurium, a priority pathogenic contaminant of milk. Common sandwich LFIA with all immunoreagents pre-applied to the test strip (format A) was compared with incubation of the sample and (gold nanoparticle—antibody) conjugate, preceding the lateral flow processes (format B), and sequential passages of the sample and the conjugate along the test strip (format C). Under the chosen conditions, the detection limits and the assay times were 3 × 104, 1 × 105, and 3 × 105 cells/mL, 10, 15, and 20 min for formats A, B, and C, respectively. The selected format A of LFIA was successfully applied to test milk samples. The sample’s dilution to a fat content of 1.0% causes pathogen detection, with 70–110% revealing and 1.5–8.5% accuracy. The obtained results demonstrate that the developed LFIA allows the detection of lower concentrations of Salmonella cells and, in this way, accelerates decision-making in food safety control.

show abstract

Integrated lateral flow immunoassays using trimethylsilyl cellulose barriers for the enhanced sensitivity of COVID-19 diagnosis

Cited by 3 publications

References 40 publications

Evaluation of serum and urine biomarkers for severe COVID-19

Evaluation of serum and urine biomarkers for severe COVID-19

Systematic optimization of UCNPs-LFA for Helicobacter pylori nucleic acid detection at point-of-care

Lateral Flow Immunosensing of Salmonella Typhimurium Cells in Milk: Comparing Three Sequences of Interactions

Contact Info

Product

Resources

About