Integrated Metabolomic and Proteomic Analysis Reveals Systemic Responses of Rubrivivax benzoatilyticus JA2 to Aniline Stress

Abstract

Aromatic amines are widely distributed in the environment and are major environmental pollutants. Although degradation of aromatic amines is well studied in bacteria, physiological adaptations and stress response to these toxic compounds is not yet fully understood. In the present study, systemic responses of Rubrivivax benzoatilyticus JA2 to aniline stress were deciphered using metabolite and iTRAQ-labeled protein profiling. Strain JA2 tolerated high concentrations of aniline (30 mM) with trace amounts of aniline being transformed to acetanilide. GC-MS metabolite profiling revealed aniline stress phenotype wherein amino acid, carbohydrate, fatty acid, nitrogen metabolisms, and TCA (tricarboxylic acid cycle) were modulated. Strain JA2 responded to aniline by remodeling the proteome, and cellular functions, such as signaling, transcription, translation, stress tolerance, transport and carbohydrate metabolism, were highly modulated. Key adaptive responses, such as transcription/translational changes, molecular chaperones to control protein folding, and efflux pumps implicated in solvent extrusion, were induced in response to aniline stress. Proteo-metabolomics indicated extensive rewiring of metabolism to aniline. TCA cycle and amino acid catabolism were down-regulated while gluconeogenesis and pentose phosphate pathways were up-regulated, leading to the synthesis of extracellular polymeric substances. Furthermore, increased saturated fatty acid ratios in membranes due to aniline stress suggest membrane adaptation. The present study thus indicates that strain JA2 employs multilayered responses: stress response, toxic compound tolerance, energy conservation, and metabolic rearrangements to aniline.

Simultaneous enhancement of phenolic compound degradations by Acinetobacter strain V2 via a step‐wise continuous acclimation process

“…Apart from morphological changes, presence of extracellular polymeric substances (Fig. 3) was also seen in response to stress as observed by other researchers [20].…”

Metabolic Exchange with Non-Alkane-Consuming Pseudomonas stutzeri SLG510A3-8 Improves n -Alkane Biodegradation by the Alkane Degrader Dietzia sp. Strain DQ12-45-1b

“…DQ12-45-1b, P. stutzeri SLG510A3-8, and their mixture were obtained by centrifugation of 2-ml samples at 4°C and 10,000 rpm for 10 min to remove C 16 -exposed cells. For desalinization, 10 ml methanol was added to each cell-free culture, vortexed for 5 min, and kept at 4°C for 5 h. The mixtures were then centrifuged at 20,000 rpm and 4°C for 10 min, and the supernatant was lyophilized (Freeze-Dryer; Boyikang, Beijing, China) for 4 h. The lyophilized samples were derivatized as described by Mujahid et al (49). GC-MS analysis of the derivatized samples was performed on a GCMS-QP2010 (Shimadzu, Japan).…”

Genomic Analysis and Stability Evaluation of the Phenol-Degrading Bacterium Acinetobacter sp. DW-1 During Water Treatment

“…Rubrivivax sp. can tolerate high concentrations of aniline ( Mujahid et al, 2015 ), and Hydrogenophaga sp. can degrade 2-butoxyethanol ( Woiski et al, 2020 ) and might be responsible for microcystin degradation ( Ding et al, 2020 ).…”