Integrated Metabolomic and Transcriptomic Analysis Reveals the Effect of Artificial Shading on Reducing the Bitter Taste of Bamboo Shoots

Huang, Yongjian; Xun, Hang; Yi, Guilin; Li, Ti; Yao, Xi; Tang, Feng

doi:10.3390/horticulturae8070594

Cited by 7 publications

(3 citation statements)

References 69 publications

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“…Coupled with mass spectroscopy (MS), LC–MS is ideally suited for detecting and quantifying multi-components in complex plant extracts [ 52 ]. Based on our previous work on the chemical constituents of bamboo plants [ 38 , 53 , 54 , 55 ], 21 phenolic compounds were selected as the extraction targets in this research. The molecular structures of the target compounds are shown in Figure S3 .…”

Section: Resultsmentioning

confidence: 99%

High-Yield Recovery of Antioxidant Compounds from Bambusa chungii Culms Using Pressurized Hot Water Extraction

et al. 2022

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A large amount of waste from Bambusa chungii culms is generated from the bamboo pulping industry, causing disposal problems. Nevertheless, bamboo culms are a suitable source of functional ingredients, such as antioxidant compounds. However, because of the high compactness and tightness in their material structure, obtaining phytochemicals from bamboo culms using conventional organic solvent extraction methods can be inefficient. In this research, we developed a pressurized hot water extraction (PHWE) method to recover 19 target phenolic compounds from Bambusa chungii culms. The extracted compounds were determined by ultra-high performance liquid chromatography–quadrupole time-of-flight–mass spectrometry (UPLC–QTOF–MS). The antioxidant potential of the extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. We investigated the effects of temperature, extraction time, and the material-to-liquid ratio on PHWE, and these parameters were optimized with a Box–Behnken design experiment and response surface tool. The optimal extraction condition was found at 170 °C, with a 1:30 g/mL material-to-liquid ratio and a 14 min extraction time. Following these optimal parameters, the total yield of target phenolic compounds (TYPC) reached 3.85 mg/g of raw material, and the half-maximal inhibitory concentrations (IC50) for the DPPH and ABTS tests were 94.7 mg/L and 21.88 mg/L, respectively. The FRAP value was 1.23 μmol FSE/mg of dried extract. A strong correlation between TYPC and the antioxidant activity of the extract was confirmed. The TYPC and antioxidant capacity of the optimal PHWE extract of the Bambusa chungii culms were both considerably higher than those of extracts obtained from conventional solvent extractions. These results indicated that PHWE is an excellent green technique for recovering phenolic compounds from bamboo culms, and the PHWE extracts of Bambusa chungii culms may be a good source of natural antioxidants.

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Section: Resultsmentioning

confidence: 99%

High-Yield Recovery of Antioxidant Compounds from Bambusa chungii Culms Using Pressurized Hot Water Extraction

et al. 2022

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“…The RNA was extracted from leaf samples using a modified CTAB method ( Kanani et al 2019 ). Reverse transcription (RT) was conducted with the Monad 1st Strand cDNA Synthesis Kit, and the qRT-PCR analysis was achieved using Tb Green® Premix Ex Taq™ II (Takara, Beijing, China) as previously described ( Huang et al 2022 , 2023 ). All samples had three biological and technical replicates.…”

Section: Methodsmentioning

confidence: 99%

Salicylic acid modulates secondary metabolism and enhanced colchicine accumulation in long yellow daylily (Hemerocallis citrina)

Miao,

Li,

Pan

et al. 2024

AoB PLANTS

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Salicylic acid (SA) is an essential phytoregulator that is widely used to promote the synthesis of high-value nutraceuticals in plants. However, its application in daylily, an ornamental plant highly valued in traditional Chinese medicine, has not been reported. Herein, we investigated the exogenous SA-induced physiological, transcriptional, and biochemical changes in long yellow daylily (LYD). We found that 2 mg/L foliar SA treatment significantly improved LYD plant growth and yield. Transcriptome sequencing and differentially expressed genes analysis revealed that the phenylpropanoid biosynthesis, isoquinoline alkaloid biosynthesis, sulfur metabolism, plant hormone signal transduction, and tyrosine metabolism were significantly induced in SA-treated leaves. Many transcription factors and antioxidant system-related DEGs were induced under the SA treatment. Biochemical analyses showed that the leaf contents of soluble sugar (SS), soluble protein (Cpr), ascorbic acid (AsA), and colchicine were significantly increased by 15.15% (from 30.16±1.301 to 34.73±0.861 mg/g), 19.54% (from 60.3±2.227 to 72.08±1.617 mg/g), 30.45% (from 190.1±4.56 to 247.98±11.652 μg/g), and 73.05% (from 3.08±0.157 to 5.33±0.462 μg/g), respectively, under the SA treatment. Furthermore, we identified 15 potential candidate genes for enhancing the growth, production, and phytochemical content of LYD. Our results provide support for the bioaccumulation of colchicine in yellow daylily and valuable resources for biotechnological-assisted production of this important nutraceutical in Hemerocallis spp.

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“…The UPLC-MS/MS analysis of anthocyanin compounds was based primarily on the detailed standard procedure described previously [44]. To determine the substrate specificity for PgF3 5 H, nine flavonoids (Table 1), which can be directly catalyzed or synthesized by F3 5 H [12,45,46], were chosen as standards for further testing.…”

Section: Extraction and Analysis Of Anthocyanins In Transformed Tobaccomentioning

confidence: 99%

Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco

Ma,

Jia,

Duan

et al. 2023

Genes

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Flavonoid-3′,5′-hydroxylase (F3′5′H) is the key enzyme for the biosynthesis of delphinidin-based anthocyanins, which are generally required for purple or blue flowers. Previously, we isolated a full-length cDNA of PgF3′5′H from Platycodon grandiflorus, which shared the highest homology with Campanula medium F3′5′H. In this study, PgF3′5′H was subcloned into a plant over-expression vector and transformed into tobacco via Agrobacterium tumefaciens to investigate its catalytic function. Positive transgenic tobacco T0 plants were obtained by hygromycin resistance screening and PCR detection. PgF3′5′H showed a higher expression level in all PgF3′5′H transgenic tobacco plants than in control plants. Under the drive of the cauliflower mosaic virus (CaMV) 35S promoter, the over-expressed PgF3′5′H produced dihydromyricetin (DHM) and some new anthocyanin pigments (including delphinidin, petunidin, peonidin, and malvidin derivatives), and increased dihydrokaempferol (DHK), taxifolin, tridactyl, cyanidin derivatives, and pelargonidin derivatives in PgF3′5′H transgenic tobacco plants by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis, resulting in a dramatic color alteration from light pink to magenta. These results indicate that PgF3′5′H products have F3′5′H enzyme activity. In addition, PgF3′5′H transfer alters flavonoid pigment synthesis and accumulation in tobacco. Thus, PgF3′5′H may be considered a candidate gene for gene engineering to enhance anthocyanin accumulation and the molecular breeding project for blue flowers.

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Integrated Metabolomic and Transcriptomic Analysis Reveals the Effect of Artificial Shading on Reducing the Bitter Taste of Bamboo Shoots

Cited by 7 publications

References 69 publications

High-Yield Recovery of Antioxidant Compounds from Bambusa chungii Culms Using Pressurized Hot Water Extraction

High-Yield Recovery of Antioxidant Compounds from Bambusa chungii Culms Using Pressurized Hot Water Extraction

Salicylic acid modulates secondary metabolism and enhanced colchicine accumulation in long yellow daylily (Hemerocallis citrina)

Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco

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