Integrated metabolomics and network analysis reveal changes in lipid metabolisms of tripterygium glycosides tablets in rats with collagen-induced arthritis

Gao, Yanhua; Qi, Qian; Xun, Ge; Zhang, Jia; Sun, Shuo; Liu, Xin; Liu, Fangfang; Ge, Jiachen; Zhang, Huaxing; Fu, Yan; Su, Suwen; Wang, Xu; Wang, Qiao

doi:10.1016/j.csbj.2023.02.050

Cited by 5 publications

(1 citation statement)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…OPLS-DA models were performed between the CG and MG and the MG and YL, respectively. The results of the OPLS-DA score plot and the 200 permutation test in serum metabolomics were presented in Figures 4A-H Frontiers in Pharmacology frontiersin.org revealed that the R 2 Y values of each model were greater than 0.997 and the Q 2 values were greater than 0.928, indicating that the classification and explanation ability of the models was excellent (Gao et al, 2023). The 200 times permutation tests suggested that the OPLS-DA models established were all reliable and not overfitting (Yang et al, 2020).…”

Section: Yl-modulated Serum Metabolomic Profilingmentioning

confidence: 99%

Metabolomics analysis delineates the therapeutic effects of Yinlan Tiaozhi capsule on triton WR-1339 -induced hyperlipidemia in mice

Xiao,

Xu,

Jiang

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

Hyperlipidemia is a disorder of lipid metabolism resulting from abnormal blood lipid metabolism and is one of the most frequent metabolic diseases that endanger people’s health. Yinlan Tiaozhi capsule (YL) is a formulated TCM widely used to treat hyperlipidemia. The purpose of this study was to discover biomarkers utilizing untargeted metabolomics techniques, as well as to analyze the mechanisms underlying the changes in metabolic pathways linked to lipid-lowering, anti-inflammation, and regulation of angiogenesis in hyperlipidemia mice. To assess the efficacy of YL, serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c) levels were measured. Biochemical examinations showed that YL significantly reduced the levels of TC, TG, LDL-c, Il6, Tnf-α, and Vegfa in hyperlipidemia mice (p < 0.01). YL also significantly increased the levels of HDL-c and Alb (p < 0.01). Twenty-seven potential serum biomarkers associated with hyperlipidemia were determined. These differential metabolites were related to the reduction of serum lipid levels in hyperlipidemia mice, probably through metabolic pathways such as linoleic acid metabolism, glycerophospholipid metabolism, phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and D-glutamine and D-glutamate metabolism. Further correlation analysis showed that the serum lipid reduction through YL was related to the metabolites (amino acid metabolites, phospholipids metabolites, and fatty acids metabolites). The present study reveals that YL has a profound effect on alleviating triton WR-1339-induced hyperlipidemia, inflammation, and angiogenesis and that the positive effects of YL were primarily associated with the correction of metabolic abnormalities and the maintenance of metabolite dynamic balance.

show abstract

Section: Yl-modulated Serum Metabolomic Profilingmentioning

confidence: 99%

Metabolomics analysis delineates the therapeutic effects of Yinlan Tiaozhi capsule on triton WR-1339 -induced hyperlipidemia in mice

Xiao,

Xu,

Jiang

et al. 2023

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

Toxic effects of Tripterygium glycoside tablets on the reproductive system of male rats by metabolomics, cytotoxicity, and molecular docking

Gao

et al. 2023

Phytomedicine

View full text Add to dashboard Cite

Metabolomics Combined with Network Pharmacology to Explore the Active Components and Mechanism of Tetrastigma planicaule (Hook.) Gagnep. in the Treatment of Rheumatoid Arthritis

Qiu,

Qin,

et al. 2024

Journal of Chemistry

View full text Add to dashboard Cite

Context. Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease. It is characterized by persistent joint damage. Traditional Chinese medicine (TCM) has demonstrated notable efficacy in managing RA. Prior investigations have indicated that the ethyl acetate extract of Tetrastigma planicaule (Hook.) Gagnep. (TP) possesses substantial anti‐inflammatory properties, suggesting the potential for screening TCM drugs with antirheumatoid arthritis attributes. However, the precise mechanism underlying its pharmacological effects and material basis remains unclear, impeding the advancement of TCM innovation. Objective. This study is to elucidate the active components and mechanism of TP in the treatment of RA. Materials and Methods. A rat model of collagen‐induced arthritis (CIA) was established for conducting pharmacological experiments to assess the effectiveness of ethyl acetate extract from TP in treating RA. In addition, nuclear magnetic resonance (NMR) metabolomics technology was employed to identify potential endogenous biomarkers for further metabolic pathway analysis. The active compounds and key targets were investigated using ultraperformance liquid chromatography‐quadrupole time‐of‐flight mass spectrometry (UPLC‐Q‐TOF‐MS/MS) technology in combination with network pharmacology. Moreover, common gene targets discovered by metabolomics and network pharmacology were validated by molecular docking with the relevant active components. Result. 12 endogenous biomarkers were screened by 1H‐NMR metabolomics technology. The metabolite pathways are primarily implicated in lipid metabolism and amino acid metabolism. 49 compounds were identified in TP, of which 10 were considered active ingredients through network pharmacological analysis. In a comprehensive analysis, it was found that TP exhibited a strong association with the PLA2G4A and lipid metabolite pathways in RA. Molecular docking studies further demonstrated a high affinity between the active compounds of TP and PLA2G4A. Discussion and Conclusions. TP may play a therapeutic role in RA by regulating PLA2G4A and participating in back‐regulating the glycerophospholipid metabolic pathway. The study elucidated the multicomponent, multitarget, and multipathway mechanism of TP in the treatment of RA, laying the groundwork for a deeper understanding of its therapeutic mechanisms.

show abstract

Integrated metabolomics and network analysis reveal changes in lipid metabolisms of tripterygium glycosides tablets in rats with collagen-induced arthritis

Cited by 5 publications

References 49 publications

Metabolomics analysis delineates the therapeutic effects of Yinlan Tiaozhi capsule on triton WR-1339 -induced hyperlipidemia in mice

Metabolomics analysis delineates the therapeutic effects of Yinlan Tiaozhi capsule on triton WR-1339 -induced hyperlipidemia in mice

Toxic effects of Tripterygium glycoside tablets on the reproductive system of male rats by metabolomics, cytotoxicity, and molecular docking

Metabolomics Combined with Network Pharmacology to Explore the Active Components and Mechanism of Tetrastigma planicaule (Hook.) Gagnep. in the Treatment of Rheumatoid Arthritis

Contact Info

Product

Resources

About