Integrated micromixer for incubation and separation of cancer cells on a centrifugal platform using inertial and dean forces

Aguirre, Gerson R.; Efremov, Vitaly; Κιτσαρά, Μαρία; Ducrée, Jens

doi:10.1007/s10404-014-1450-7

Cited by 32 publications

(29 citation statements)

References 44 publications

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“…This method was effective in mixing fluorescent tags with targeted cancer cells in a blood sample. 83 Other passive mixing methods use droplets as microreaction chambers 84 or bubbles to promote chaotic advection. 85 Droplet formation on a disc, in which the small diameter of the microreaction chambers significantly reduces the diffusion distance of any reactants, has been demonstrated by Haeberle et al 84 Bubble mixing using T-junctions has not yet been integrated on a disc, possibly due to the constraint of the disc's footprint size, but it could be implemented in the future.…”

Section: Mixingmentioning

confidence: 99%

“…Aguirre et al used Dean forces in serial serpentine flow-focusing channels to separate cell-bead complexes from blood. 83 Morijiri et al separated particles of different densities and sizes using a rotational movement combined with a technique called pinched-flow fractionation, as illustrated in Figure 17. The fluidic structure was filled with a bulk buffer solution before the particles were introduced.…”

Section: Particle Sortingmentioning

confidence: 99%

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Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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The field of centrifugal microfluidics has experienced tremendous growth during the past 15 years, especially in applications such as lab-on-a-disc (LoD) diagnostics. The strength of LoD systems lies in its potential for development into fully integrated sample-to-answer analysis systems. This review highlights the technologies necessary to develop the next generation of these systems. In addition to outlining valving and other fluid-handling operations, we discuss the recent advances and future outlook in four categories of LoD processes: reagent storage, sample preparation, nucleic acid amplification, and analyte detection strategies.

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Section: Mixingmentioning

confidence: 99%

Section: Particle Sortingmentioning

confidence: 99%

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

et al. 2016

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“…37,38 Carboxylated microbeads are also recently utilized for specific biomolecular capture and extraction in a centrifugal platform. 39,40 However, high-resolution separation or extraction of particles in the centrifugal system is typically complicated, 9 and often requires adding density gradient media 41 or other active labeling methods. 32 Increasingly, there is also a pressing need to develop diagnostic tools that utilize low sample volumes in a high throughput and cost effective manner.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic size separation of cells and particles using a swinging bucket centrifuge

2015

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Biomolecular separation is crucial for downstream analysis. Separation technique mainly relies on centrifugal sedimentation. However, minuscule sample volume separation and extraction is difficult with conventional centrifuge. Furthermore, conventional centrifuge requires density gradient centrifugation which is laborious and time-consuming. To overcome this challenge, we present a novel size-selective bioparticles separation microfluidic chip on a swinging bucket minifuge. Size separation is achieved using passive pressure driven centrifugal fluid flows coupled with centrifugal force acting on the particles within the microfluidic chip. By adopting centrifugal microfluidics on a swinging bucket rotor, we achieved over 95% efficiency in separating mixed 20 μm and 2 μm colloidal dispersions from its liquid medium. Furthermore, by manipulating the hydrodynamic resistance, we performed size separation of mixed microbeads, achieving size efficiency of up to 90%. To further validate our device utility, we loaded spiked whole blood with MCF-7 cells into our microfluidic device and subjected it to centrifugal force for a mere duration of 10 s, thereby achieving a separation efficiency of over 75%. Overall, our centrifugal microfluidic device enables extremely rapid and label-free enrichment of different sized cells and particles with high efficiency.

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“…The rotationally induced 'artificial gravity' field can be harnessed for manipulation of cells and bioparticles [4]. In combination with hydrodynamic forces, Aguirre et al [5] separated different size range of bioparticles. Kirby et al [6] applied centrifugo-magnetophoresis for the extraction of MCF7 cells from a background of whole blood.…”

Section: Introductionmentioning

confidence: 99%

SIZE- and deformability-based particle sorting by strategic design of obstacle arrays in continuous centrifugal sedimentation mode

Nwankire

Maguire

Kernan

et al. 2015

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

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This paper describes a novel, smart-grid technique for isolating, sorting and capturing cancer cells based on size and deformability by lateral displacement. In stopped-flow, centrifugal sedimentation mode, the bioparticles are sizeselectively displaced along the dynamically spaced grid of the microstructures in a deterministic fashion. Following displacement, particles are captured in claw-like structures for onward processing. This displacement method was first demonstrated for sorting seven bead populations with diameters between 5 µm and 30 µm from a mixture. Then HeLa and melanoma cells spiked in buffer and whole blood were isolated and captured. Finally, we succeeded to sort normal and fixed HeLa cells according to their deformability.

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Integrated micromixer for incubation and separation of cancer cells on a centrifugal platform using inertial and dean forces

Cited by 32 publications

References 44 publications

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

Lab-on-a-CD: A Fully Integrated Molecular Diagnostic System

Microfluidic size separation of cells and particles using a swinging bucket centrifuge

SIZE- and deformability-based particle sorting by strategic design of obstacle arrays in continuous centrifugal sedimentation mode

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