Integrated miRNA and mRNA analysis in gills of spotted sea bass reveals novel insights into the molecular regulatory mechanism of salinity acclimation

Wang, Lingyu; Zhang, Xiaoyan; Wen, Haishen; Qi, Xin; Sun, Donglei; Li, Xueqi; Li, Jinku; Tian, Yuan; Zhang, Kaiqiang; Li, Yun

doi:10.1016/j.aquaculture.2023.739778

Cited by 8 publications

(2 citation statements)

References 55 publications

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“…Previous studies have extensively documented the ability of miRNAs to regulate multiple target mRNAs, and reciprocally, mRNA can be regulated by multiple miRNAs [ 29 , 30 ]. Numerous known miRNAs involving in various processes have been identified in different fish species under different types of stressors [ 23 , 24 ]. However, in our study, we observed that most of the DEMs associated with glucose metabolism (novel-miR-240, novel-miR-241, novel-miR-176, novel-miR-194, etc.)…”

Section: Discussionmentioning

confidence: 99%

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Integrated mRNA and miRNA analysis reveals the regulatory network of oxidative stress and inflammation in Coilia nasus brains during air exposure and salinity mitigation

Gao,

Mang,

Liu

et al. 2024

BMC Genomics

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Background Air exposure is an inevitable source of stress that leads to significant mortality in Coilia nasus. Our previous research demonstrated that adding 10‰ NaCl to aquatic water could enhance survival rates, albeit the molecular mechanisms involved in air exposure and salinity mitigation remained unclear. Conversely, salinity mitigation resulted in decreased plasma glucose levels and improved antioxidative activity. To shed light on this phenomenon, we characterized the transcriptomic changes in the C. nasus brain upon air exposure and salinity mitigation by integrated miRNA-mRNA analysis. Results The plasma glucose level was elevated during air exposure, whereas it decreased during salinity mitigation. Antioxidant activity was suppressed during air exposure, but was enhanced during salinity mitigation. A total of 629 differentially expressed miRNAs (DEMs) and 791 differentially expressed genes (DEGs) were detected during air exposure, while 429 DEMs and 1016 DEGs were identified during salinity mitigation. GO analysis revealed that the target genes of DEMs and DEGs were enriched in biological process and cellular component during air exposure and salinity mitigation. KEGG analysis revealed that the target genes of DEMs and DEGs were enriched in metabolism. Integrated analysis showed that 24 and 36 predicted miRNA-mRNA regulatory pairs participating in regulating glucose metabolism, Ca2+ transport, inflammation, and oxidative stress. Interestingly, most of these miRNAs were novel miRNAs. Conclusion In this study, substantial miRNA-mRNA regulation pairs were predicted via integrated analysis of small RNA sequencing and RNA-Seq. Based on predicted miRNA-mRNA regulation and potential function of DEGs, miRNA-mRNA regulatory network involved in glucose metabolism and Ca2+ transport, inflammation, and oxidative stress in C. nasus brain during air exposure and salinity mitigation. They regulated the increased/decreased plasma glucose and inhibited/promoted antioxidant activity during air exposure and salinity mitigation. Our findings would propose novel insights to the mechanisms underlying fish responses to air exposure and salinity mitigation.

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Section: Discussionmentioning

confidence: 99%

“…MicroRNAs (miRNAs) are a group of non-coding endogenous small RNAs, typically 21-25 nucleotides in length, that play crucial roles in the response of fish to environmental stress [23][24][25]. Through interactions with target genes, miRNAs modulate cellular functions and metabolism by regulating gene expression levels [26].…”

Section: Discussionmentioning

confidence: 99%