Integrated mRNA and microRNA transcriptome analyses provide insights into paclobutrazol inhibition of lateral branching in herbaceous peony

Abstract

Herbaceous peony (Paeonia lactiflora Pall.) is a new high-end cut flower, but a large number of lateral branches often appear in some excellent cultivars, which is inconvenient for cut flower production. In the present study, we analyzed the effects of paclobutrazol (PBZ) on the lateral branches of P. lactiflora and adopted a next-generation sequencing approach to identify miRNAs and mRNAs that were differentially expressed involved in the PBZ response. Our results indicate that PBZ may inhibit the production of lateral branches on P. lactiflora. There were 827 differentially expressed genes (DEGs) and 104 differentially expressed miRNAs (DEMs). Integrative analysis revealed 29 miRNA-mRNA interactions related to PBZ stress. Our results provided a wealth of genetic information and data on metabolic pathways for revealing the regulatory mechanism of PBZ inhibition of the development of lateral branches in P. lactiflora and provided a new possibility for reducing lateral branch formation in the production of herbaceous peony cut flowers.

Location Model of Fresh Agricultural Products Logistics Distribution Center Based on Network Nodes

“…Because of the importance of choosing the location of the fresh agricultural product logistics distribution center, more and more scholars and researchers have invested in this area, and have achieved quite good results [9]. Sangirova puts forward a method above speed for fresh farm products.…”

“…Equation 19 shows that when 0ijw =, supply point i does not provide alternative distribution center j, when 1ijw =, supply point i provides alternative distribution center j. Formula 20 indicates that the number of backup distribution centers delivered to the demand point is not less than the demand point; Formula (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) indicates that the performance balance of each backup distribution center is guaranteed. Formula 21 indicates that both the delivery volume and delivery distance of fresh produce are non-negative numbers.…”

Integrated analysis of miRNAome transcriptome and degradome reveals miRNA-target modules governing floral florescence development and senescence across early- and late-flowering genotypes in tree peony

“…Prediction of miRNAs in tree peony have been reported, including miRNAs in response to copper stress ( Jin et al., 2015 ), involved in bud dormancy release ( Zhang et al., 2018 ), seed fatty acid synthesis ( Yin et al., 2018 ), flower spot formation ( Zhao et al., 2019 ), petal variegation ( Shi et al., 2019 ), flower development ( Han et al., 2020 ), and brassinolide treatment on flowering ( Zhang et al., 2022 ). In herbaceous peony, miRNAs involved in response to stress from high temperature ( Hao et al., 2017 ) and Botrytis cinerea infection ( Zhao et al., 2015 ), as well as lateral branch formation ( Liu et al., 2020 ) have been predicted. In addition, miR156e-3p of herbaceous peony has been proved to enhance anthocyanin accumulation in lateral branches of transgenic Arabidopsis thaliana ( Zhao et al., 2017 ).…”