Integrated network analyses identify MYB4R1 neofunctionalization in the UV-B adaptation of Tartary buckwheat

Liu, Moyang; Sun, Wenjun; Ma, Zhaotang; Guo, Chaocheng; Wu, Qi; Wang, Xiyin; Hui, Chi Man

doi:10.1016/j.xplc.2022.100414

Cited by 17 publications

(8 citation statements)

References 91 publications

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“…Previous studies have found that MYB4 serves as a repressor for flavonoid biosynthesis and that MYB4 expression is down-regulated and can enhance sinapate ester levels in Arabidospis leaves with exposure to UV-B light [ 48 ]. In buckwheat, in response to UV-B, MYB4R1 regulates flavonoid and anthocyanin biosynthesis by binding to L box motifs in the promoter of CHS , FLS , and UFGT [ 49 ]. Moreover, the MYB-bHLH-WD40 complex can regulate genes that encode late step enzymes in the pathway, leading to increased flavonoid biosynthesis [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Light-Induced Flavonoid Biosynthesis in Sinopodophyllum hexandrum with High-Altitude Adaptation

Zhao

Dong

et al. 2023

Plants

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Sinopodophyllum hexandrum is a perennial alpine herb producing the anti-cancer metabolite podophyllotoxin (PPT). Although the adaptation of S. hexandrum to high altitudes has been demonstrated and the effects of temperature, precipitation, and UV-B light on plant growth and metabolite accumulation have been studied, knowledge on the role of flavonoid biosynthesis in adapting to high altitudes is limited. In this study, light intensity, amount and type of flavonoids, and differentially expressed proteins (DEPs) and genes (DEGs) at 2300 and 3300 m were analyzed by HPLC, proteomic, transcriptomic, and qRT-PCR analysis. We found that higher light intensity correlated with greater flavonoid, flavonol, and anthocyanin content as well as higher anthocyanin to total flavonoid and flavonol ratios observed at the higher altitude. Based on proteomic and transcriptomic analyses, nine DEPs and 41 DEGs were identified to be involved in flavonoid biosynthesis and light response at 3300 m. The relative expression of nine genes (PAL, CHS1, IFRL, ANS, MYB4, BHLH137, CYP6, PPO1, and ABCB19) involved in flavonoid biosynthesis and seven genes (HSP18.1, HSP70, UBC4, ERF5, ERF9, APX3, and EX2) involved in light stress were observed to be up-regulated at 3300 m compared with 2300 m. These findings indicate that light intensity may play a regulatory role in enhancing flavonoid accumulation that allows S. hexandrum to adapt to elevated-altitude coupled with high light intensity.

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Section: Discussionmentioning

confidence: 99%

Light-Induced Flavonoid Biosynthesis in Sinopodophyllum hexandrum with High-Altitude Adaptation

Zhao

Dong

et al. 2023

Plants

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“…It was demonstrated that F. esculentum exhibits anti-hypertension, anti-diabetic, anti-inflammatory, cardio-protective, hepato-protective, and neuroprotective properties [ 7 , 8 ]. Tartary buckwheat exhibits higher than common buckwheat resistance to high altitudes, harsh weather conditions such as drought, frost, UV-B radiation damage, and pests [ 9 ]. Additionally, compared to common buckwheat, F. tataricum contains more rutin and other phenolics and flavonoids, with well-balanced amino acids, making it a functional pseudocereal [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…DNA methylation is also involved in forming double flowers and dichromatic petals [ 37 , 38 ]. Moreover, it plays a crucial role in the expression of genes related to anthocyanin pigmentation in flower tissue [ 9 , 39 , 40 ]. Existing evidence suggests that methylation alterations accompany the flowering process in plants.…”

Section: Introductionmentioning

confidence: 99%

DNA methylation analysis of floral parts revealed dynamic changes during the development of homostylous Fagopyrum tataricum and heterostylous F. esculentum flowers

Sala-Cholewa,

Tomasiak,

Nowak

et al. 2024

BMC Plant Biol

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Background Proper flower development is essential for plant reproduction, a crucial aspect of the plant life cycle. This process involves precisely coordinating transcription factors, enzymes, and epigenetic modifications. DNA methylation, a ubiquitous and heritable epigenetic mechanism, is pivotal in regulating gene expression and shaping chromatin structure. Fagopyrum esculentum demonstrates anti-hypertensive, anti-diabetic, anti-inflammatory, cardio-protective, hepato-protective, and neuroprotective properties. However, the heteromorphic heterostyly observed in F. esculentum poses a significant challenge in breeding efforts. F. tataricum has better resistance to high altitudes and harsh weather conditions such as drought, frost, UV-B radiation damage, and pests. Moreover, F. tataricum contains significantly higher levels of rutin and other phenolics, more flavonoids, and a balanced amino acid profile compared to common buckwheat, being recognised as functional food, rendering it an excellent candidate for functional food applications. Results This study aimed to compare the DNA methylation profiles between the Pin and Thrum flower components of F. esculentum, with those of self-fertile species of F. tataricum, to understand the potential role of this epigenetic mechanism in Fagopyrum floral development. Notably, F. tataricum flowers are smaller than those of F. esculentum (Pin and Thrum morphs). The decline in DNA methylation levels in the developed open flower components, such as petals, stigmas and ovules, was consistent across both species, except for the ovule in the Thrum morph. Conversely, Pin and Tartary ovules exhibited a minor decrease in DNA methylation levels. The highest DNA methylation level was observed in Pin stigma from closed flowers, and the most significant decrease was in Pin stigma from open flowers. In opposition, the nectaries of open flowers exhibited higher levels of DNA methylation than those of closed flowers. The decrease in DNA methylation might correspond with the downregulation of genes encoding methyltransferases. Conclusions Reduced overall DNA methylation and the expression of genes associated with these epigenetic markers in fully opened flowers of both species may indicate that demethylation is necessary to activate the expression of genes involved in floral development.

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“…The yield of salvianolic acid B (SAB) of the Sichuan ecotype is significantly higher than that of the Shandong ecotype, while the accumulation of tanshinone IIA is relatively low, which indicates its potential stronger tolerance to oxidative stress. , However, with advancements in industrialization, the cultivated soil in the Sichuan Province faces a huge risk of antibiotic pollution. , Tetracyclines, which are used as antibiotics in animals and include doxycycline (DOX), enrich the soil in large quantities annually through animal manure. − This increases the potential risk of antibiotic enrichment in the roots of the S. miltiorrhiza Sichuan ecotype and seriously endangers the production safety of downstream drug processing and pharmaceutical development. , More importantly, most environmental stresses cause varying degrees of damage to plant mitochondria, including but not limited to cold, high temperatures, drought, UV-B radiation, heavy metals, etc. As a type of protein functional inhibitor that directly targets mitochondria, DOX is an excellent model for studying environmental issues with mitochondrial damage as the hub .…”

Section: Introductionmentioning

confidence: 99%

“…11−13 This increases the potential risk of antibiotic enrichment in the roots of the S. miltiorrhiza Sichuan ecotype and seriously endangers the production safety of downstream drug processing and pharmaceutical development. 9,10 More importantly, most environmental stresses cause varying degrees of damage to plant mitochondria, including but not limited to cold, 14 high temperatures, 15 drought, 16 UV-B radiation, 17 heavy metals, 18 etc. As a type of protein functional inhibitor that directly targets mitochondria, DOX is an excellent model for studying environmental issues with mitochondrial damage as the hub.…”

Section: Introductionmentioning

confidence: 99%

Integrated Multiomics and Synergistic Functional Network Revealed the Mechanism in the Tolerance of Different Ecotypes ofSalvia miltiorrhizaBge. to Doxycycline Pollution

Zhang

Yang

et al. 2023

Environ. Sci. Technol.

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Tetracycline pollution in soil irreversibly damages the biosafety of plants by inhibiting the mitochondrial function. Some traditional Chinese medicine (TCM) plants, such as Salvia miltiorrhiza Bunge, have a strong tolerance to mitochondrial damage. We comprehensively compared the doxycycline (DOX) tolerances of two ecotypes of S. miltiorrhiza in the Sichuan and Shandong provinces and found that the Sichuan ecotype had a lower yield reduction, more stable accumulation of medicinal ingredients, higher mitochondrial integrity, and a more robust antioxidant system. The synergetic response networks under DOX pollution of both ecotypes were constructed using RNA sequencing and ultrahigh-performance liquid chromatography−tandem mass spectrometry. The differentiation of the downstream pathways of aromatic amino acids (AAAs) produced variations in the DOX tolerance of S. miltiorrhiza in different regions. The Sichuan ecotype maintained redox homeostasis and xylem development by activating salvianolic acid and indole biosynthesis, while the Shandong ecotype balanced chemical and mechanical defenses by regulating the flavonoid biosynthesis. Rosmarinic acid, a downstream AAA molecule, maintains the mitochondrial homeostasis of plant seedlings under DOX pollution by targeting the ABCG28 transporter. We also highlight the significance of downstream AAA small molecules in guiding the development of bio-based environmental pollution remediation agents.

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Integrated network analyses identify MYB4R1 neofunctionalization in the UV-B adaptation of Tartary buckwheat

Cited by 17 publications

References 91 publications

Light-Induced Flavonoid Biosynthesis in Sinopodophyllum hexandrum with High-Altitude Adaptation

Light-Induced Flavonoid Biosynthesis in Sinopodophyllum hexandrum with High-Altitude Adaptation

DNA methylation analysis of floral parts revealed dynamic changes during the development of homostylous Fagopyrum tataricum and heterostylous F. esculentum flowers

Integrated Multiomics and Synergistic Functional Network Revealed the Mechanism in the Tolerance of Different Ecotypes ofSalvia miltiorrhizaBge. to Doxycycline Pollution

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