Integrated optical biochemical sensor fabricated using rapid-prototyping techniques

Chang-Yen, David A.; Gale, Bruce K.

doi:10.1117/12.478164

Cited by 9 publications

(3 citation statements)

References 30 publications

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“…To verify the effectiveness of the gas control from the gas channel and calibration of the oxygen concentration in the cell channel, a fluorescence dye, tris (2,20-bipyridyl dichlororuthenium) hexahydrate (Ru(bpy)) (Sigma-Aldrich) was used. Its fluorescence intensity is inversely proportional to the dissolved oxygen concentration . The cell channel was filled with DI water containing Ru(bpy).…”

Section: Methodsmentioning

confidence: 99%

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Electrical Impedance Characterization of Erythrocyte Response to Cyclic Hypoxia in Sickle Cell Disease

et al. 2019

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Cell sickling is the process in which intracellular polymerization of deoxygenated sickle hemoglobin (HbS) leads to distorted, rigid cells, resulting in abnormal blood rheology and painful vaso-occlusion. Current methods for detection of this process mainly rely on optical microscopy of cellular morphology and measurements of cell deformability and blood rheology. As electrical impedance of cells is a sensitive indicator of changes in cellular structure and biophysical characteristics, it can be a promising marker for characterization of abnormal blood rheology and a means more convenient than optics to be integrated into point-of-care devices. In this work, a microfluidics-based electrical impedance sensor has been developed for characterizing the dynamic cell sickling–unsickling processes in sickle blood. The sensor is capable of measuring the continuous variation in the sickle cell suspension due to cyclic hypoxia-induced intracellular HbS polymerization and depolymerization. Simultaneous microscopic imaging of cell morphological change shows the reliability and repeatability of the electrical impedance-based measurements of cell sickling and unsickling processes. Strong correlation is found between the electrical impedance measurement and patients’ hematological parameters such as levels of HbS and fetal hemoglobin. The combination of electrical impedance measurement and on-chip hypoxia control provides a promising method for rapid assessment of the dynamic processes of cell sickling and unsickling in patients with sickle cell disease.

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Section: Methodsmentioning

confidence: 99%

“…Its fluorescence intensity is inversely proportional to the dissolved oxygen concentration. 35 The cell channel was filled with DI water containing Ru(bpy). Gray values (red curve) converted from the fluorescence intensity of Ru(bpy) were plotted as a function of time (Figure 2).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Electrical Impedance Characterization of Erythrocyte Response to Cyclic Hypoxia in Sickle Cell Disease

et al. 2019

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“…33,44 Several biosensors, chemical, and optical sensors have been developed to allow real-time measurements to detect cells, proteins, glucose, DNA, and analyte molecules of interest. 10,16,59,85 Several excellent reviews on fiber optic sensors, electrochemical sensors, and cell-based biosensors are made available in published literature. 11,62,77 The purpose of this review is to delineate the various sensing modalities used in the development of devices for specific use in monitoring engineered tissue growth, particularly for applications in scaffold based tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Enabling Sensor Technologies for the Quantitative Evaluation of Engineered Tissue

Starly

Choubey

2007

Ann Biomed Eng

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Research in regenerative medicine has necessitated the need for advanced sensing technologies to monitor and evaluate the quality of engineered tissues. Several sensing schemes have been developed to sense specific analytes that enable researchers to assess tissue morphology, growth, and function. In addition to microscopy and staining techniques, tissue engineers are presented with an array of optical, chemical, and biological sensor technologies, which provide them with an opportunity to monitor variables, such as oxygen concentration, pH value, carbon dioxide, and glucose concentration in a noninvasive or minimally invasive manner. The article presents a short description on the core technologies and research reviews on the use of sensors employed in tissue engineering over the past decade. The article concludes by presenting some of the challenges to the further development of these technologies that are capable of real time measurement of tissue structure, composition, and function both for in-vitro and in-vivo analysis.

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Laser Micro and Nano Processing of Metals , Ceramics , and Polymers

Pfleging

Kohler

Südmeyer

et al. 2012

Laser-Assisted Fabrication of Materials

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Integrated optical biochemical sensor fabricated using rapid-prototyping techniques

Cited by 9 publications

References 30 publications

Electrical Impedance Characterization of Erythrocyte Response to Cyclic Hypoxia in Sickle Cell Disease

Electrical Impedance Characterization of Erythrocyte Response to Cyclic Hypoxia in Sickle Cell Disease

Enabling Sensor Technologies for the Quantitative Evaluation of Engineered Tissue

Laser Micro and Nano Processing of Metals , Ceramics , and Polymers

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