Integrated system for combined Raman spectroscopy–spectral domain optical coherence tomography

Patil, Chetan A.; Kalkman, Jeroen; Faber, Dirk J.; Nyman, Jeffry S.; Leeuwen, Ton G. van; Mahadevan‐Jansen, Anita

doi:10.1117/1.3520132

Cited by 51 publications

(45 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Angle-resolved elastic scattering has been used to measure the sizes of sub-cellular organelles [154,155]. Other groups have looked at tissue structure using optical coherence tomography (OCT) [71,156]. In each of these cases, the Raman spectroscopy part of the instrument required little or no alteration to be combined with the structurally-specific technique.…”

Section: Combinations With Other Techniquesmentioning

confidence: 99%

See 1 more Smart Citation

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

270

189

View full text Add to dashboard Cite

Raman spectroscopy is an increasingly popular technique in many areas including biology and medicine.It is based on Raman scattering, a phenomenon in which incident photons lose or gain energy via interactions with vibrating molecules in a sample. These energy shifts can be used to obtain information regarding molecular composition of the sample with very high accuracy. Applications of Raman spectroscopy in the life sciences have included quantification of biomolecules, hyperspectral molecular imaging of cells and tissue, medical diagnosis, and others. This review briefly presents the physical origin of Raman scattering explaining the key classical and quantum mechanical concepts. Variations of the Raman effect will also be considered, including resonance, coherent, and enhanced Raman scattering. We discuss the molecular origins of prominent bands often found in the Raman spectra of biological samples. Finally, we examine several variations of Raman spectroscopy techniques in practice, looking at their applications, strengths, and challenges. This review is intended to be a starting resource for scientists new to Raman spectroscopy, providing theoretical background and practical examples as the foundation for further study and exploration.

show abstract

Section: Combinations With Other Techniquesmentioning

confidence: 99%

“…Ko et al used this strategy to investigate dental caries [444]. Patil et al used the same spectrograph and CCD to detect both OCT and Raman signals [71]. Their instrument was used to detect skin cancer [445].…”

Section: Applicationsmentioning

confidence: 99%

Raman spectroscopy: techniques and applications in the life sciences

Shipp¹,

Sinjab²,

Notingher³

2017

Adv. Opt. Photon.

270

189

View full text Add to dashboard Cite

show abstract

“…A MPT/confocal laser scanning microscopy system was used to investigate various dermatological diseases such as actinic keratosis and stucco keratosis [51]. Similarly, clinical trials using OCT in combination with imaging techniques such as Raman spectroscopy, coherent anti-Stokes Raman spectroscopy, photoacoustic tomography and fluorescence spectroscopy has been demonstrated and these multimodal systems were able to provide complimentary structural and functional information [52][53][54][55]. Recently, a multimodal optical microscope incorporating OCM, MPT and FLIM have been designed and developed to obtain images of human skin in vivo [56].…”

Section: Discussionmentioning

confidence: 99%

Three‐dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

Alex

Weingast

Weinigel

et al. 2012

Journal of Biophotonics

View full text Add to dashboard Cite

A preliminary clinical trial using state‐of‐the‐art multiphoton tomography (MPT) and optical coherence tomography (OCT) for three‐dimensional (3D) multimodal in vivo imaging of normal skin, nevi, scars and pathologic skin lesions has been conducted. MPT enabled visualization of sub‐cellular details with axial and transverse resolutions of <2 μm and <0.5 μm, respectively, from a volume of 0.35 × 0.35 × 0.2 mm3 at a frame rate of 0.14 Hz (512 × 512 pixels). State‐of‐the‐art OCT, operating at a center wavelength of 1300 nm, was capable of acquiring 3D images depicting the layered architecture of skin with axial and transverse resolutions ∼8 μm and ∼20 μm, respectively, from a volume of 7 × 3.5 × 1.5 mm3 at a frame rate of 46 Hz (1024 × 1024 pixels). This study demonstrates the clinical diagnostic potential of MPT/OCT for pre‐screening relatively large areas of skin using 3D OCT to identify suspicious regions at microscopic level and subsequently using high resolution MPT to obtain zoomed in, sub‐cellular level information of the respective regions (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

“…110 The Raman scattering process itself can be generated by two-photon processes 111 although it is generally much easier to implement with the use of nanostructures 112 or by the addition of nanoparticles to generate surface-enhanced Raman scattering (SERS). 113,114 Due to their similar measurement con¯gurations (laser scanning with detection of backscattered light), Raman and OCT have been combined in order to retrieve both the 3D structure and the vibrational spectrum, either with point-measurements in Raman, 115,116 or with imaging in both modalities through sample scanning. 117 As OCT employs wide-band light sources to generate optical sectioning, an extension denoted as spectral OCT (S-OCT) consists in resolving spectrally the measurement to obtain both the 3D structure through OCT, as well as the absorption spectra.…”

Section: Spectroscopic Multimodal Implementationsmentioning

confidence: 99%

Multimodal label-free microscopy

Pavillon

Fujita

Smith

2014

J. Innov. Opt. Health Sci.

View full text Add to dashboard Cite

This paper reviews the di®erent multimodal applications based on a large extent of label-free imaging modalities, ranging from linear to nonlinear optics, while also including spectroscopic measurements. We put speci¯c emphasis on multimodal measurements going across the usual boundaries between imaging modalities, whereas most multimodal platforms combine techniques based on similar light interactions or similar hardware implementations. In this review, we limit the scope to focus on applications for biology such as live cells or tissues, since by their nature of being alive or fragile, we are often not free to take liberties with the image acquisition times and are forced to gather the maximum amount of information possible at one time. For such samples, imaging by a given label-free method usually presents a challenge in obtaining su±cient optical signal or is limited in terms of the types of observable targets. Multimodal imaging is then particularly attractive for these samples in order to maximize the amount of measured information. While multimodal imaging is always useful in the sense of acquiring additional information from additional modes, at times it is possible to attain information that could not be discovered using any single mode alone, which is the essence of the progress that is possible using a multimodal approach.

show abstract

Integrated system for combined Raman spectroscopy–spectral domain optical coherence tomography

Cited by 51 publications

References 35 publications

Raman spectroscopy: techniques and applications in the life sciences

Raman spectroscopy: techniques and applications in the life sciences

Three‐dimensional multiphoton/optical coherence tomography for diagnostic applications in dermatology

Multimodal label-free microscopy

Contact Info

Product

Resources

About