Integrated transcriptome sequencing and RNA interference reveals molecular changes in <i>Diaphorina citri</i> after exposure to validamycin

Yu, Hai‐Zhong; Xie, Yan-Xin; Wang, Jie; Wang, Ying; Du, Yu‐Zhou; Wang, Hegui; Zhong, Balian; Zhu, Bo; Xj, Yu; Lu, Zhanjun

doi:10.1111/1744-7917.12880

Cited by 6 publications

(1 citation statement)

References 58 publications

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“…For instance, inhibiting Tre activity by validamycin reduced the expression levels of chitin metabolism-related genes and led to a malformed Diaphorina citri (Hemiptera, Psyllidae) phenotype [ 30 ]. Moreover, silencing of the chitinase-like protein ENO3 and CP7 genes in Diaphorina citri by RNAi resulted in abnormal phenotypes [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Validamycin Inhibits the Synthesis and Metabolism of Trehalose and Chitin in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Liu

et al. 2023

Insects

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The oriental fruit fly, Bactrocera dorsalis (Hendel), is a notorious invasive pest that has raised concerns worldwide. Validamycin has been demonstrated to be a very strong inhibitor against trehalase in a variety of organisms. However, whether validamycin can inhibit trehalase activity to suppress trehalose hydrolysis and affect any other relevant physiological pathways in B. dorsalis remains unknown. In this study, the effects of validamycin injection on the synthesis and metabolism of trehalose and chitin were evaluated. The results show that validamycin injection significantly affected trehalase activity and caused trehalose accumulation. In addition, the downstream pathways of trehalose hydrolysis, including the synthesis and metabolism of chitin, were also remarkably affected as the expressions of the key genes in these pathways were significantly regulated and the chitin contents were changed accordingly. Intriguingly, the upstream trehalose synthesis was also affected by validamycin injection due to the variations in the expression levels of key genes, especially BdTPPC1. Moreover, BdTPPC1 was predicted to have a binding affinity to validamycin, and the subsequent in vitro recombinant enzyme activity assay verified the inhibitory effect of validamycin on BdTPPC1 activity for the first time. These findings collectively indicate that validamycin can be considered as a promising potential insecticide for the management of B. dorsalis.

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Section: Introductionmentioning

confidence: 99%

Validamycin Inhibits the Synthesis and Metabolism of Trehalose and Chitin in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Liu

et al. 2023

Insects

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Characterization of an RR‐2 cuticle protein DcCP8 and its potential application based on SPc nanoparticle‐wrapped dsRNA in Diaphorina citri

Lu,

Xia,

Zhang

et al. 2024

Pest Management Science

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BACKGROUNDThe insect cuticle consists of chitin fibers and a protein matrix, which plays an important role in protecting the body from invasion of various pathogens and prevents water loss. Periodic synthesis and degradation of the cuticle is required for the growth and development of insects. Key genes involved in cuticle formation have long been considered a potential target for pest control.RESULTSIn this study, a member of the RR‐2 subfamily of cuticular protein 8 (DcCP8) was identified from the Diaphorina citri genome database. Immunofluorescence analysis suggested that DcCP8 was mainly located in the Diaphorina citri exocuticle and can be induced to up‐regulate 12 h following 20‐hydroxyecdysone (20E) treatment. Silencing of DcCP8 by RNA interference (RNAi) significantly disrupted the metamorphosis to the adult stage, and improved the permeability of the cuticle. Transmission electron microscopy (TEM) analysis revealed that the synthesis of the exocuticle was impressed after silencing of DcCP8. Furthermore, the recombinant DcCP8 protein exhibited chitin‐binding properties in vitro, down‐regulation of DcCP8 significantly inhibited expression levels of chitin metabolism‐related genes. Additionally, a sprayable RNAi method targeting DcCP8 based on star polycation (SPc) nanoparticles‐wrapped double‐stranded RNA (dsRNA) significantly increased Diaphorina citri mortality. Transcriptome sequencing further confirmed that genes associated with the endocytic pathway and immune response were up‐regulated in Diaphorina citri after SPc treatment.CONCLUSIONSThe current study indicated that DcCP8 is critical for the formation of Diaphorina citri exocuticles, and lays a foundation for Diaphorina citri control based on large‐scale dsRNA nanoparticles. © 2024 Society of Chemical Industry.

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Novel bacterial plasmid produces small interfering RNAs (siRNAs) that induce effective gene silencing in the Asian citrus psyllid Diaphorina citri

Rueda-Silva,

González-Campos,

Durán-Armenta

et al. 2023

Electronic Journal of Biotechnology

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Integrated transcriptome sequencing and RNA interference reveals molecular changes in Diaphorina citri after exposure to validamycin

Cited by 6 publications

References 58 publications

Validamycin Inhibits the Synthesis and Metabolism of Trehalose and Chitin in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Validamycin Inhibits the Synthesis and Metabolism of Trehalose and Chitin in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Characterization of an RR‐2 cuticle protein DcCP8 and its potential application based on SPc nanoparticle‐wrapped dsRNA in Diaphorina citri

Novel bacterial plasmid produces small interfering RNAs (siRNAs) that induce effective gene silencing in the Asian citrus psyllid Diaphorina citri

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