Integrated transcriptomic and proteomic analysis of the acetic acid stress in Issatchenkia orientalis

Abstract

Issatchenkia orientalis known as a multi-tolerant non-Saccharomyces yeast, which tolerant environmental stresses, exhibits potential in wine making and bioethanol production. It is essential for the growth of I. orientalis to tolerant acetic acid in the mixed cultures with Saccharomyces cerevisiae. In this work, RNA-sequence and TMT (Tandem Mass Tag) were used to examine the comprehensive transcriptomic and proteomic profiles of I. orientalis in response to acetic acid. The results showed that 876 genes were identified differentially transcribed in I. orientalis genome and 399 proteins expressed in proteome after 4 hr acetic acid (90 mM, pH 4.5). The comprehensive analysis showed a series of determinants of acetic acid tolerance: Glycolysis and TCA cycle provide enough nicotinamide adenine dinucleotide to effectively convert acetic acid.Genes associated with potassium, iron, zinc, and glutathione synthesis were upregulated. The same changes of differentially expressed genes and proteins were mainly concentrated in chaperones, coenzyme, energy production, and transformation. Practical applicationsIn addition to the main fermentation products, wine yeast also produces metabolite acetic acid in the fermentation process, and yeast cells are exposed to acetic acid stress, which restrains cell proliferation. Issatchenkia orientalis exhibits great potential in winemaking and bioethanol production. The yeast is known as a multi-tolerant non-Saccharomyces yeast that can tolerate a variety of environmental stresses. In this study, RNA-Seq and TMT were conducted to investigate the changes in transcriptional and proteomic profile of I. orientalis under acetic acid stress. The knowledge of the transcription and expression changes of the I. orientalis is expected to understand the tolerance mechanisms in I. orientalis and to guide traditional fermentation processes by Saccharomyces cerevisiae improving its high resistance to acetic acid stress. K E Y W O R D Sacetic acid stress, Issatchenkia orientalis, proteomic, RNA-sequence, TMT, transcriptomic

Interaction profile of a mixed-culture fermentation of Issatchenkia orientalis and Saccharomyces cerevisiae by transcriptome sequencing

“…Among them, Hsp82p is a death-promoting molecule related to apoptosis induced by acetic acid (Silva et al , 2013). Our results are consistent with published results that heat shock proteins Hsp70 and Hsp90 were significantly upregulated under acetic acid stress in I.orientalis (Li et al , 2020).…”

Acetic acid stress response of the acidophilic sulfate reducer Acididesulfobacillus acetoxydans

“…Although not differentially expressed, kdpABC and the other cation transporters could also contribute to the relief of osmotic stress caused by acetic acid spiking (Mira et al, 2010;Wood, 2015). Whereas often acetic acid tolerance mechanisms resolve around pH homeostasis (Li et al, 2020;Trček et al, 2015), this study shows that in A. acetoxydans there is no pronounced proton stress response following acetic acid spiking.…”

Effect of Dissolved Oxygen and Acid on the Yield of 2,3-butanediol by Saccharomyces Cerevisiae W141

“…Hog1p can bind to and phosphorylate the channel protein Fps1p, causing it to undergo endocytosis and reducing the entry of acetic acid into the cell [43]. Increasing evidence shows that determinants of acetic acid tolerance allow glycolysis and the TCA cycle to provide su cient nicotinamide adenine dinucleotide (NADH) [16]. The experimental results described above further indicate that acid stress and the amount of dissolved oxygen regulate 2,3-BDO biosynthesis in yeast by altering the intracellular redox balance.…”

“…Interestingly, disrupting the NADH/NAD + balance, which affects the intracellular redox balance, has been shown to promote the synthesis of 2,3-BDO in S. cerevisiae [9,12,14,15]. However, S. cerevisiae commonly encounters acid stress in industrial settings, which will greatly affect the redox balance in the cell and metabolite yields [16][17][18]. The acid stress response mechanism of yeast cells includes the following four aspects: (1) the cell membrane maintains the stability of intracellular pH by restricting the penetration of high levels of acid; (2) the cell membrane channel proteins that regulates transcription factors and acid transport maintains the intracellular stabilization of pH; (3) adenosine triphosphatase H + -ATPase (encoded by the PMA1 gene) on the cell membrane can hydrolyse ATP to produce energy, pumping protons out of the cell to maintain a normal neutral pH environment in the cell; and (4) maintaining the integrity and uidity of cell membranes by modulating fatty acid composition [13,19,20].…”